A5000615064- Extracellular vesicles in disease
- Metabolomics and Mass Spectrometry Studies
- MicroRNA in disease regulation
- Metabolism and Genetic Disorders
- Multiple Sclerosis Research Studies
- Advanced Proteomics Techniques and Applications
- Ocular Surface and Contact Lens
- Glaucoma and retinal disorders
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Mass Spectrometry Techniques and Applications
- Cardiac Fibrosis and Remodeling
- Neuroinflammation and Neurodegeneration Mechanisms
- Biotin and Related Studies
- Pancreatic function and diabetes
- Chronic Myeloid Leukemia Treatments
- Pancreatic and Hepatic Oncology Research
- Neonatal Health and Biochemistry
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Endoplasmic Reticulum Stress and Disease
- Cytokine Signaling Pathways and Interactions
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Colorectal Cancer Surgical Treatments
University of Chieti-Pescara
2016-2025
Institute for Biomedicine
2014
Centro Studi Industriali
2007-2010
Ospedale SS. Annunziata
2006-2009
Istituti di Ricovero e Cura a Carattere Scientifico
2007
Tecnologie Avanzate (Italy)
2007
Bambino Gesù Children's Hospital
2007
Fondazione Santa Lucia
2007
Abstract Aims Sodium-glucose cotransporter 2 inhibitors have beneficial effects on heart failure and cardiovascular mortality in diabetic non-diabetic patients, with unclear mechanisms. Autophagy is a cardioprotective mechanism under acute stress conditions, but excessive autophagy accelerates myocardial cell death leading to autosis. We evaluated the protective role of empagliflozin (EMPA) against cardiac injury murine cardiomyopathy. Methods results Male mice, rendered diabetics by one...
Multiple Sclerosis (MuS) is a complex multifactorial neuropathology, resulting in heterogeneous clinical presentation. A very active MuS research field concerns the discovery of biomarkers helpful to make an early and definite diagnosis. The sphingomyelin pathway has emerged as molecular mechanism involved MuS, since high levels ceramides cerebrospinal fluid (CSF) were related axonal damage neuronal dysfunction. Ceramides are hydrolysis products sphingomyelins through reaction catalyzed by...
Chimeric Antigen Receptor (CAR) T cell-derived Extracellular Vesicles (EVs) might represent a new therapeutic tool for boosting CAR-T cell antileukemic effects. Here, cohort of 22 patients infused with CD19.CAR-T cells was monitored the presence circulating CD19.CAR+-T EVs (CD19.CAR+EVs), which were then separated and functionally characterized their killing abilities. A GMP-compliant separation method also developed. Results demonstrated that CD19.CAR+EVs detectable in peripheral blood up...
Abstract Background Mass spectrometry protein profiling is a promising tool for biomarker discovery in clinical proteomics. However, the development of reliable approach separation signals from noise required. In this paper, LIMPIC, computational method detection peaks linear-mode MALDI-TOF data proposed. LIMPIC based on novel techniques background reduction and baseline removal. Peak performed considering presence non-homogeneous level mass spectrum. A comparison collected multiple spectra...
Abstract Urinary exosomes are released from every renal epithelial cell type facing the urinary space and therefore, they may carry molecular markers of dysfunction structural injury. Here, we present a hyphenated microLC‐Q‐TOF‐MS platform for lipidomics studies applied to investigate exosome lipid repertoire. Lipids were separated by reversed‐phase chromatography using linear gradient formic acid 0.2% tetrahydrofuran, in 40 min analysis. Features ( m/z with associated own retention time)...
Oleuropein, the main phenolic compound in virgin olive oil, and several of its derivatives such as oleuropein aglycone, hydroxytyrosol, their respective acetylated lipophilic forms were obtained by simple environmentally friendly semisynthetic protocols. The same molecules then tested vitro vivo, comparing intriguing anti-COX-1 anti-COX-2 properties to those well-known anti-inflammatory drugs ibuprofen celecoxib. Finally, molecular modeling experiments displaying most probable binding modes...
Phytochemicals constitute a heterogeneous group of substances with an evident role in human health. Their properties on cancer initiation, promotion and progression are well documented. Particular attention is now devoted to better understand the molecular basis their anticancer action. In present work, we studied effect resveratrol ovarian cell line OVCAR-3 by proteomic approach. Our findings demonstrate that down-regulates protein cyclin D1 and, concentration dependent manner,...
Primary open angle glaucoma (POAG) is one of the main causes irreversible blindness worldwide. The pathogenesis POAG still unclear. Alteration and sclerosis trabecular meshwork with changes in aqueous humor molecular composition seem to play key role. Increased intraocular pressure widely known be risk factor for onset progression disease. Unfortunately, early diagnosis remains challenge. In order provide insight into patho-physiology glaucoma, here we report a shotgun proteomics approach...
(1) Lipid profiling in MuS and OND patients. (2) Search of alterations associated with MuS. (3) Characterization differences.
Primary open-angle glaucoma (POAG) represents the leading cause of irreversible blindness worldwide and is a multifactorial, chronic neurodegenerative disease characterized by retinal ganglion cell visual field loss. There are many factors that associated with risk developing POAG, increased intraocular pressure being one most prevalent. Due to asymptomatic nature disease, diagnosis POAG often occurs too late, which necessitates development new effective screening strategies for early...
Metabolomics based on mass spectrometry represents an innovative approach to characterize multifactorial diseases, such as multiple sclerosis (MuS). To date, the most important biomarker source for MuS diagnosis is cerebrospinal fluid. However, goal research identify new molecules in more easily accessible biological fluids. A very interesting biofluid represented by tears, considered intermediate fluid between and serum. In this work, we developed a merged strategy analysis of lipids...
Extracellular vesicles (EVs) are released by shedding during different physiological processes and increasingly thought to be new potential biomarkers. However, the impact of pre-analytical processing phases on final measurement is not predictable for this reason, translation basic research into clinical practice has been precluded. Here we have optimized a simple procedure in combination with polychromatic flow cytometry (PFC), identify, classify, enumerate, separate circulating EVs from...
Newborn screening (NBS) for inborn errors of metabolism is one the most advanced tools secondary prevention in medicine, as it allows early diagnosis and prompt treatment initiation. The expanded newborn was introduced Italy between 2016 2017 (Law 167/2016; DM 13 October 2016; DPCM 12-1-2017). A total 1,586,578 infants born were screened January December 2020. For this survey, we collected data from 15 Italian laboratories, focusing on metabolic disorders identified by tandem mass...
Primary open angle (POAG) and pseudoexfoliative glaucoma (PXG) are the most common primary secondary forms of glaucoma, respectively. Even though patho-physiology, aqueous humor composition, risk factors, clinical features, therapy drug induced ocular surface changes in POAG PXG have been widely studied, to date information concerning tear protein characterization is lacking. Tears a source nourishment for tissues vehicle remove local waste products, metabolized drugs inflammatory mediators...
The recent introduction of the “precision medicine” concept in oncology pushed cancer research to focus on dynamic measurable biomarkers able predict responses novel anticancer therapies order improve clinical outcomes. Recently, involvement extracellular vesicles (EVs) pathophysiology has been described, and given their release from all cell types under specific stimuli, EVs have also proposed as potential cancer. Among techniques used study EVs, flow cytometry a high potential. Here, we...