A5102777417- Ultrasound Imaging and Elastography
- Ultrasound and Hyperthermia Applications
- Photoacoustic and Ultrasonic Imaging
- Ultrasonics and Acoustic Wave Propagation
- Flow Measurement and Analysis
- Cardiovascular Health and Disease Prevention
- Acute Myeloid Leukemia Research
- Ultrasound and Cavitation Phenomena
- Electrical and Bioimpedance Tomography
- Advanced MRI Techniques and Applications
- Coronary Interventions and Diagnostics
- Epigenetics and DNA Methylation
- Cardiac Imaging and Diagnostics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Biometric Identification and Security
- Acute Lymphoblastic Leukemia research
- Hemodynamic Monitoring and Therapy
- Lung Cancer Treatments and Mutations
- DNA Repair Mechanisms
- Cytokine Signaling Pathways and Interactions
- Lymphoma Diagnosis and Treatment
- Kruppel-like factors research
- Cerebrovascular and Carotid Artery Diseases
- Lung Cancer Research Studies
- Glutathione Transferases and Polymorphisms
University of Florence
2015-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2000-2024
Università Cattolica del Sacro Cuore
2010-2024
Institut d'Astrophysique de Paris
2024
Agostino Gemelli University Polyclinic
2012-2024
University of Bologna
2011
École Nationale Supérieure de Techniques Avancées
2011
Catholic University of America
2011
Azienda-Unita' Sanitaria Locale Di Cesena
2011
Ospedale Regionale di Locarno
2009
Cancer stem cells (CSCs) are a subpopulation of cancer endowed with high tumorigenic, chemoresistant and metastatic potential. Nongenetic mechanisms acquired resistance increasingly being discovered, but molecular insights into the evolutionary process CSCs limited. Here, we show that type I interferons (IFNs-I) function as hubs during immunogenic chemotherapy, triggering epigenetic regulator demethylase 1B (KDM1B) to promote an adaptive, yet reversible, transcriptional rewiring towards...
The experimental test of novel ultrasound (US) investigation methods can be made difficult by the lack flexibility commercial US machines. In best options, these only provide beamformed radiofrequency or demodulated echo-signals for acquisition an external PC. More is achieved in high-level research platforms, but are typically characterized high cost and large size. This paper presents a powerful portable system, specifically developed purposes. system design has been based on integrated...
Open scanners offer an increasing support to the ultrasound researchers who are involved in experimental test of novel methods. Each system presents specific performance terms number channels, flexibility, processing power, data storage capability, and overall dimensions. This paper reports design criteria hardware/software implementation details a new 256-channel advanced open platform. is organized modular architecture, including multiple front-end boards, interconnected by high-speed (80...
The availability of programmable and reconfigurable ultrasound (US) research platforms may have a considerable impact on the advancement systems technology; indeed, they allow novel transmission strategies or challenging processing methods to be tested experimentally refined. In this paper, ULtrasound Advanced Open Platform (ULA-OP), recently developed in our University laboratory, is shown flexible tool that can easily adapted wide range applications. Five nonstandard working modalities are...
Polycythemia vera (PV) and essential thrombocythemia (ET) can present in pediatric age as sporadic or familial diseases. To define the biologic profile of childhood PV ET, we evaluated specific markers a cohort patients affected by including cases with occurrence.Thirty-eight children ET were investigated. The control group included 58 adults ET. Endogenous erythroid colonies, qualitative reverse transcriptase polymerase chain reaction for polycythemia rubra vera-1 (PRV-1) RNA expression,...
High frame rate (HFR) imaging methods based on the transmission of defocused or plane waves rather than focused beams are increasingly popular. However, production HFR images poses severe requirements both in and reception sections ultrasound scanners. In particular, major technical difficulties arise if must be continuously produced real-time, i.e., without any acquisition interruption nor loss data. This paper presents implementation real-time HFR-compounded application ULA-OP 256 research...
Glutathione S-transferase P1 (GSTP1) is a member of the GST enzyme superfamily that important for detoxification several cytotoxic drugs and their by-products. A single nucleotide polymorphism results in substitution isoleucine (Ile) to valine (Val) at codon 105, causing metabolically less active variant enzyme. We assessed impact GSTP1 105 genotype on treatment outcome patients with Hodgkin's lymphoma.The Ile(105)Val gene was analyzed using PCR-RFLP technique. Ninety-seven lymphoma were...
BRCA1 plays a pivotal role in the repair of DNA damage, especially following chemotherapy and ionising radiation. We were interested regulation expression acute myeloid leukaemia (AML), particular therapy-related forms (t-AML). Using real-time PCR Western blot, we found that mRNA was expressed at barely detectable levels by normal peripheral blood granulocytes, monocytes lymphocytes, whereas control BM-mononuclear cells selected CD34+ progenitor displayed significantly higher (P=0.0003)....
Abstract In polycythemia vera (PV) and essential thrombocythemia (ET) specific JAK2 mutations constitutively activate the JAK‐STAT pathway, explaining biologic findings such as endogenous erythroid colony (EECs) growth or PRV‐1 RNA overexpression. Since these markers are detected also in wild type patients, we hypothesized that, cases, activation of pathway could be produced by a deregulation suppressor cytokine signaling (SOCS) protein system. Eighty‐one patients with PV ET (53 adults 28...
Medical ultrasound contrast agents (UCAs) have evolved from straight image enhancers to pathophysiological markers and drug delivery vehicles. However, the exact dynamic behavior of encapsulated bubbles composing UCAs is still not entirely known. In this article, we propose characterize full populations UCAs, by looking at translational effects radiation force on each bubble in a diluted population. The setup involves sensitive, fully programmable transmitter/receiver two unconventional,...
Epigenetic changes play a role in the pathogenesis of myeloid malignancies, and hypomethylating agents have shown efficacy these diseases. We studied apoptotic effect, genome-wide methylation, gene expression profiles HL60 cells following 5-aza-2′-deoxycytidine (decitabine; DAC) treatment, using microarray technologies. Decitabine treatment resulted decrease global DNA corresponding to 4876 probeset IDs with significantly reduced methylation levels, while 2583 was modified. The integrated...
Ultrasound contrast agents (UCA) populations are typically polydisperse and contain microbubbles with radii over a given range. Although the behavior of certain sizes might be masked by others, acoustic characterization UCA is made on full populations. In this paper, we have combined optical methods to investigate response isolated lipid-shelled low-pressure (49 62 kPa peak negative pressure) ultrasound tone bursts. These bursts induced slow deflation microbubbles. The experimental setup...
Ultrasound contrast agent (UCA) imaging provides a cost-effective diagnostic tool to assess tissue perfusion and vascular pathologies. However, excessive transmission (TX) levels may negatively impact both uniform diffusion survival rates of agents, limiting their density thus echogenicity. Contrast detection methods with high sensitivity low-contrast destruction rate are essential maintain capabilities. Plane-wave TX number compounding angles has been suggested produce good quality images...
Irisin, a recently discovered myokine, has been considered prognostic factor in several cardiovascular diseases. Nevertheless, no data are available on the role of irisin patients with heart failure (HF), both preserved (HFpEF) or reduced (HFrEF) ejection fraction. We have therefore evaluated circulating levels HFpEF and HFrEF patients, correlating them metabolic parameters total antioxidant capacity (TAC), as index oxidative stress. Irisin was significantly higher than (7.72 ± 0.76 vs 2.77...
The blood–brain barrier (BBB) poses a significant challenge to drug delivery the brain. A promising approach involves low-frequency, low-intensity pulsed ultrasound (US) waves combined with intravenously injected microbubbles (MB) temporarily and non-invasively open BBB. However, current technologies cannot easily integrate this procedure US imaging. Passive cavitation detection, tracing harmonic emissions of MB during sonication, has been preferred method for real-time monitoring...
The operation of a novel ultrasound multigate instrument capable computing in real-time the fast Fourier transform (FFT) Doppler signals detected from 64 equally spaced range cells is presented. new system provides up to 50 velocity profiles per second, which are displayed such manner that information about full spectral content at all investigated depths continuously monitored over PRF-wide frequency can be set arbitrarily between -PRF and +PRF. Experimental results presented, demonstrate...