A5066526290- Viral gastroenteritis research and epidemiology
- Autophagy in Disease and Therapy
- Virus-based gene therapy research
- Cell death mechanisms and regulation
- Phagocytosis and Immune Regulation
- Cytomegalovirus and herpesvirus research
- Mitochondrial Function and Pathology
- Infant Nutrition and Health
- HIV Research and Treatment
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Lipid Membrane Structure and Behavior
- Herpesvirus Infections and Treatments
- RNA Interference and Gene Delivery
- Respiratory viral infections research
- Clostridium difficile and Clostridium perfringens research
- Viral-associated cancers and disorders
- Lymphoma Diagnosis and Treatment
- ATP Synthase and ATPases Research
- Yersinia bacterium, plague, ectoparasites research
- Cervical Cancer and HPV Research
- IL-33, ST2, and ILC Pathways
Istituto Superiore di Sanità
2014-2025
National Research Council
2019
Sapienza University of Rome
2000-2010
Centro de Investigación Biomédica en Red
2010
University of Rome Tor Vergata
1998
University of Modena and Reggio Emilia
1995
The phenomenon of cell cannibalism, which generally refers to the engulfment cells within other cells, was described in malignant tumors, but its biological significance is still largely unknown. In present study, we investigated occurrence, vivo relevance, and underlying mechanisms cannibalism human melanoma. As first evidence, observed that tumor clearly detectable metastatic lesions melanoma often involved T could be found a degraded state cells. Then, vitro experiments confirmed property...
Mitochondria-associated membranes (MAMs) are subdomains of the endoplasmic reticulum (ER) that interact with mitochondria. This membrane scrambling between ER and mitochondria appears to play a critical role in earliest steps autophagy. Recently, lipid microdomains, i.e. rafts, have been identified as further actors autophagic process. In present work, series biochemical molecular analyses has carried out human fibroblasts specific aim characterizing rafts MAMs decipher their possible...
Axonal degeneration in peripheral nerves after injury is accompanied by myelin degradation initiated Schwann cells (SCs). These activate autophagy, a ubiquitous cytoprotective process essential for and recycling of cellular constituents. Concomitantly to nerve insult axonal degeneration, neuropathic pain (NeP) arises. The role SC autophagy the mechanisms underlying NeP still unknown. In this study, we examined during early phase Wallerian induction chronification using murine model lesion...
Galectins have emerged as a novel family of immunoregulatory proteins implicated in T cell homeostasis. Recent studies showed that galectin-1 (Gal-1) plays key role tumor-immune escape by killing antitumor effector cells. Here we found Gal-1 sensitizes human resting cells to Fas (CD95)/caspase-8-mediated death. Furthermore, this protein triggers an apoptotic program involving increase mitochondrial membrane potential and participation the ceramide pathway. In addition, induces coalescence,...
Crohn's disease (CD) and ulcerative colitis (UC), known as inflammatory bowel diseases (IBD), are characterized by an abnormal immunological response to commensal bacteria colonizing intestinal lumen mucosa. Among the latter, strains of adherent-invasive Escherichia coli (AIEC), capable adhering invading epithelium, replicate in macrophages, have been described CD adults. We aimed at identifying characterizing AIEC pediatric IBD.In all, 24 children, 10 UC, 23 controls were investigated....
Sphingolipids are structural lipid components of cell membranes, including membrane organelles, such as mitochondria or endoplasmic reticulum, playing a role in signal transduction well the transport and intermixing membranes. Sphingolipid microdomains, also called rafts, participate several metabolic catabolic processes, apoptosis. However, defined rafts autophagic flux is still unknown. In present study we analyzed gangliosides, class sphingolipids, autolysosome morphogenesis human murine...
Eosinophils are major effectors of Th2-related pathologies, frequently found infiltrating several human cancers. We recently showed that eosinophils play an essential role in anti-tumor responses mediated by immunotherapy with the ‘alarmin’ intereukin-33 (IL-33) melanoma mouse models. Here, we analyzed mechanisms which IL-33 mediates tumor infiltration and antitumor activities eosinophils. show recruits indirectly, via stimulation cell-derived chemokines, while it activates directly,...
A biochemical and morphological investigation of the mechanism entry vesicular stomatitis virus (VSV) into host cells mammalian (HeLa), avian (CER), piscine (EPC) arthropod (Aedes albopictus) origin, is described. VSV was capable infecting all cell lines tested by a endosome- and/or lysosome-dependent step since ammonium chloride amantadine blocked early stages infection. Complement-dependent immune lysis infected provided evidence that in none four different types examined did insertion...
Abstract Objective Estrogens influence many physiologic processes and are also implicated in the development or progression of numerous diseases, including autoimmune disorders. Aberrations lymphocyte homeostasis that lead to production multiple pathogenic autoantibodies, autoantibodies specific estrogen receptor (ER), have been detected peripheral blood patients with systemic lupus erythematosus (SLE). This study was undertaken assess presence both anti‐ERα anti‐ERβ antibodies sera from...
The phytopathogen Pseudomonas syringae pv. actinidiae ( Psa ) is the causal agent of bacterial canker kiwifruit. In last years, it has caused severe economic losses to Actinidia spp. cultivations, mainly in Italy and New Zealand. Conventional strategies adopted did not provide adequate control infection. Phage therapy may be a realistic safe answer urgent need for novel antibacterial agents aiming this pathogen. study, we described isolation characterization two bacteriophages able...
2-Deoxy-D-glucose (2DG) is a synthetic glucose analogue that inhibits glycolysis and blocks cancer cell growth. In this report, we evaluated the role of 2DG in induction death human metastatic melanoma cells. We have also examined effects combined treatments with four different pro-apoptotic agents: (i) Temozolomide (TMZ), chemotherapic drug commonly used to treat melanoma, (ii) Pyrimethamine (Pyr), antifolate recently reappraised therapy, (iii) Cisplatin (CisPt), capable directly binding...
It has been demonstrated that α-synuclein can aggregate and contribute to the pathogenesis of some neurodegenerative diseases it is capable hindering autophagy in neuronal cells. Here, we investigated implication process primary human T lymphocytes. We provide evidence that: (i) knocking down gene resulted increased autophagy, (ii) induction by energy deprivation was associated with a significant decrease levels, (iii) inhibition 3-methyladenine or ATG5 led increase (iv) impairment,...
ABSTRACT We recently demonstrated that lactoferrin, an antimicrobial glycoprotein, can inhibit adenovirus infection by competing for common glycosaminoglycan receptors. This study further characterizes the antiadenovirus activity of protein, thus demonstrating lactoferrin neutralizes binding to particles and its targets are viral III IIIa structural polypeptides.
Diesel exhaust particles (DEP) are major constituents of ambient air pollution and their adverse health effect is an area intensive investigations. With respect to the immune system, DEP have attracted significant research attention as a factor that could influence allergic diseases interfering with cytokine production chemokine expression. this exception, scant data available on impact lymphocyte homeostasis. Here, effects nanoparticles from Euro 4 (E4) 5 (E5) light duty diesel engines...
// Marina Pierdominici 1, * , Angela Maselli 2, Silvia L. Locatelli 3 Laura Ciarlo 2 Giuseppa Careddu Mario Patrizio Barbara Ascione Antonella Tinari 4 Carmelo Carlo-Stella Walter Malorni Paola Matarrese ** Elena Ortona 1 Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy Therapeutic Research Medicine Evaluation, Oncology Hematology, Humanitas Cancer Center - Clinical Center, Milano, Technology Health, These authors have contributed equally to this work...
Abstract Immune cell-derived extracellular vesicles (EV) affect tumor progression and hold promise for therapeutic applications. Eosinophils are major effectors in Th2-related pathologies recently implied cancer. Here, we evaluated the anti-tumor activities of eosinophil-derived EV following activation with alarmin IL-33. We demonstrate that IL-33-activated mouse human eosinophils produce higher quantities respect to stimulated IL-5. Following incorporation from (Eo33-EV), but not...