A5043261491- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- interferon and immune responses
- Prostate Cancer Treatment and Research
- Erythropoietin and Anemia Treatment
- Lipid metabolism and disorders
- Parkinson's Disease Mechanisms and Treatments
- Tendon Structure and Treatment
- Heat shock proteins research
- Cystic Fibrosis Research Advances
- Viral Infections and Vectors
- melanin and skin pigmentation
- Retinal and Optic Conditions
- Immune Cell Function and Interaction
- Plant Surface Properties and Treatments
- Mycobacterium research and diagnosis
- Hippo pathway signaling and YAP/TAZ
- Inflammasome and immune disorders
- Genomics and Chromatin Dynamics
- Dialysis and Renal Disease Management
- Galectins and Cancer Biology
- Cell death mechanisms and regulation
- Cellular transport and secretion
University of Rome Tor Vergata
2016-2025
Instituto Nazionale Tumori Regina Elena
2009
Tumori Foundation
2009
Istituto Pasteur
2002
Transglutaminase type 2 (TG2) is a multifunctional enzyme that plays key role in mitochondria homeostasis under stressful cellular conditions. TG2 interactome analysis reveals an interaction with GRP75 (glucose-regulated protein 75). localizes mitochondria-associated membranes (MAMs) and acts as bridging molecule between the two organelles by assembling IP3R-GRP75-VDAC complex, which involved transport of Ca2+ from endoplasmic reticulum (ER) to mitochondria. We demonstrate occurs MAMs. The...
The identification of biomarkers that distinguish between aggressive and indolent forms prostate cancer (PCa) is crucial for diagnosis treatment. In this study, we used cultured cells derived from tissue patients with PCa to define a molecular mechanism underlying the most form involves functional activation eNOS HIFs in association estrogen receptor β (ERβ). Cells poor prognosis exhibited constitutively hypoxic phenotype increased NO production. Upon treatment, formation ERβ/eNOS,...
Autophagy is a highly conserved cellular process responsible for the degradation of long-lived proteins and organelles. occurs at low levels under normal conditions, but it enhanced in response to stress, e.g. nutrient deprivation, hypoxia, mitochondrial dysfunction infection. "Tissue" transglutaminase (TG2) accumulates, both vivo vitro, high cells stressful conditions. Therefore, this study, we investigated whether TG2 could also play role autophagic process. To end, used knockout mice cell...
Cystic fibrosis (CF), the most common lethal monogenic disease in Caucasians, is characterized by recurrent bacterial infections and colonization, mainly Pseudomonas aeruginosa, resulting unresolved airway inflammation. CF caused mutations gene coding for cystic transmembrane conductance regulator (CFTR) protein, which functions as a chloride channel epithelial cells, macrophages, other cell types. Impaired handling macrophages feature of airways, although it still debated how defective CFTR...
Mitochondria-associated ER membranes (MAMs) are formed by close and specific components in the contact sites between endoplasmic reticulum (ER) mitochondria, which participate several cell functions, including lipid metabolism, autophagy, Ca2+ signaling. Particularly, presence of α-synuclein (α-syn) MAMs was previously demonstrated, indicating a physical interaction among some proteins this region potential involvement dysfunctions. alterations associated with neurodegenerative diseases such...
Abstract The histopathologic and molecular heterogeneity of prostate cancer the limited availability human tumor tissue make unraveling mechanisms carcinogenesis a challenging task. Our goal was to develop an ex vivo model that could be reliably used define prognostic signature based on gene expression profiling cell cultures maintained phenotype. To this end, we derived epithelial from explanted 59 patients undergoing radical prostatectomy or cistoprostatectomy because benign...
Transglutaminase type II (TG2) is a pleiotropic enzyme that exhibits various activities unrelated to its originally identified functions. Apart from post-translational modifications of proteins (peculiar the transglutaminase family enzymes), TG2 involved in diverse biological functions, including cell death, signaling, cytoskeleton rearrangements, displaying enzymatic activities, G-protein and non-enzymatic It variety human diseases such as celiac disease, diabetes, neurodegenerative...
Host-directed therapies are emerging as a promising tool in the curing of difficult-to-treat infections, such those caused by drug-resistant bacteria. In this study, we aim to test potential activity FDA- and EMA-approved drugs cysteamine cystamine against Mycobacterium abscessus. human macrophages (differentiated THP-1 cells), these restricted M. abscessus growth similar that achieved amikacin. Here, use ex vivo granuloma-like structures (GLS) model infection with rough (MAB-R) smooth...
Abstract TG2 is a multifunctional enzyme involved in several cellular processes and has emerging as potential regulator of gene expression. In this regard, we have recently shown that able to activate HSF1, the master transcriptional stress‐responsive genes; however, its effect on overall expression remains unclear. To address point, analyzed, by RNA-seq, transcriptome well characterized interactome nucleus. The data obtained from these omics approaches reveal markedly influences profile...
Abstract We have recently shown that type 2 transglutaminase (TG2) plays a key role in the host’s inflammatory response during bacterial infections. In this study, we investigated whether enzyme is involved regulation of STING pathway, which main signaling activated presence both self- and pathogen DNA cytoplasm, leading to I IFN (IFN I) production. demonstrated TG2 negatively regulates by impairing IRF3 phosphorylation bone marrow–derived macrophages, isolated from wild-type knockout mice....
Abstract The receptor interacting protein kinase 3 (RIPK3) is the main player in activation of necroptosis, a pro-inflammatory regulated cell death modality induced by many different stimuli. RIPK3 epigenetically DNA methylation and can be expressed when its promoter associated with H3K4me3 histone. In this study, we show that Transglutaminase 2 (TG2) necessary to induce necroptosis pathway allowing expression Ripk3 gene. Indeed, cells lacking TG2 strong downregulation gene are resistant...
Abstract Non-alcoholic fatty liver disease (NAFLD) is one of the most important diseases worldwide. Currently, no effective treatment available, and NAFLD pathogenesis incompletely understood. Transglutaminase type 2 (TG2) a ubiquitous enzyme whose dysregulation implicated in various human diseases. Here we examined impact TG2 on progression using high-fat-diet-induced model both wild-type TG2-deficient mice. Animals were fed with standard chow diet or high-fat (42% energy from fat) for 16...
Autophagy is a self-degradative physiological process by which the cell removes worn-out or damaged components. Constant at basal level it may become highly active in response to cellular stress. The type 2 transglutaminase (TG2), accumulates under stressful conditions, plays an important role regulation of autophagy and cells lacking this enzyme display impaired autophagy/mitophagy consequent shift their metabolism glycolysis. To further define molecular partners TG2 involved these...