A5068896566- Systemic Lupus Erythematosus Research
- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- Salivary Gland Disorders and Functions
- Rheumatoid Arthritis Research and Therapies
- Monoclonal and Polyclonal Antibodies Research
- Atherosclerosis and Cardiovascular Diseases
- Autoimmune and Inflammatory Disorders Research
- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- Systemic Sclerosis and Related Diseases
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Galectins and Cancer Biology
- T-cell and B-cell Immunology
- Calcium signaling and nucleotide metabolism
- Platelet Disorders and Treatments
- Lipid metabolism and disorders
- Medical and Biological Sciences
- Adenosine and Purinergic Signaling
- Erythrocyte Function and Pathophysiology
- Diabetes and associated disorders
- Blood disorders and treatments
Sapienza University of Rome
2016-2025
Istituto Superiore di Sanità
2009-2025
University of Pavia
2024
San Salvatore Hospital
2023
Policlinico Umberto I
2019-2022
University of Sassari
2010-2013
San Raffaele University of Rome
2013
National and Kapodistrian University of Athens
1997
University of Naples Federico II
1991
Autophagy, the cytoprotection mechanism that takes place under metabolic impairment, has been implicated in pathogenesis of autoimmunity. Here, we investigated spontaneous and induced autophagic behavior T lymphocytes from patients with systemic lupus erythematosus (SLE) compared healthy donors by measuring autophagy marker microtubule-associated protein 1 light chain 3 (LC3)-II. No significant differences were found between SLE donors, apart CD4+ naive cells which constitutively higher...
Abstract A variety of environmental agents has been found to influence the development autoimmune diseases; in particular, studies investigating potential association systemic rheumatic diseases with micro and nano-particulate matter are very few contradictory. In this study, role diesel exhaust particles (DEPs), one most important components environment particulate matter, emitted from Euro 4 5 engines altering Normal Human Bronchial Epithelial (NHBE) cell biological activity was evaluated....
// Marina Pierdominici 1 , Angela Maselli 2 Barbara Varano 3 Cristiana Barbati Paola Cesaro 4 Cristiano Spada Angelo Zullo 5 Roberto Lorenzetti Marco Rosati 6 Gabriella Rainaldi Maria Rosaria Limiti Luisa Guidi 7 Lucia Conti 3,* and Sandra Gessani Department of Cell Biology Neurosciences, Istituto Superiore di Sanità, Rome, Italy Therapeutic Research Medicines Evaluation, Hematology, Oncology Molecular Medicine, Digestive Endoscopy Unit, Catholic University, Gastroenterology...
Abstract Objective Estrogens influence many physiologic processes and are also implicated in the development or progression of numerous diseases, including autoimmune disorders. Aberrations lymphocyte homeostasis that lead to production multiple pathogenic autoantibodies, autoantibodies specific estrogen receptor (ER), have been detected peripheral blood patients with systemic lupus erythematosus (SLE). This study was undertaken assess presence both anti‐ERα anti‐ERβ antibodies sera from...
2-Deoxy-D-glucose (2DG) is a synthetic glucose analogue that inhibits glycolysis and blocks cancer cell growth. In this report, we evaluated the role of 2DG in induction death human metastatic melanoma cells. We have also examined effects combined treatments with four different pro-apoptotic agents: (i) Temozolomide (TMZ), chemotherapic drug commonly used to treat melanoma, (ii) Pyrimethamine (Pyr), antifolate recently reappraised therapy, (iii) Cisplatin (CisPt), capable directly binding...
Antibodies against carbamylated proteins (anti-CarP) have been recently identified in the sera of patients with rheumatoid arthritis (RA). The objective study was to evaluate prevalence, sensitivity and specificity anti-CarP compared anti-citrullinated peptide antibodies (ACPA) factor (RF), replicating existing data a large cohort Italian RA extending evaluation other autoimmune rheumatic diseases (AIRDs). Serum samples (n = 607) from 309 RA, 200 disease controls 98 normal healthy subjects...
Endothelial dysfunction has been detected in RA patients and seems to be reversed by control of inflammation. Low circulating endothelial progenitor cells (EPCs) have described many conditions associated with increased cardiovascular risk, including RA. The aim this study was investigate the effect inhibition TNF on EPCs patients. Seventeen moderate-severe 12 sex age-matched controls were evaluated. biomarkers tested at baseline after 3 months. identified from peripheral blood mononuclear...
Current evidence indicates that estrogens, in particular 17β-estradiol (E2), play a crucial role the gender bias of autoimmune diseases although underlying molecular mechanisms have not yet been fully elucidated. Immune cells estrogen receptors (ERs), i.e., ERα and ERβ, pro- anti-inflammatory functions, respectively, presence one receptor (ER) subtype over other might change effects, promoting or dampening inflammation. In this study, we contributed to define influences E2 on T from female...
It has been demonstrated that α-synuclein can aggregate and contribute to the pathogenesis of some neurodegenerative diseases it is capable hindering autophagy in neuronal cells. Here, we investigated implication process primary human T lymphocytes. We provide evidence that: (i) knocking down gene resulted increased autophagy, (ii) induction by energy deprivation was associated with a significant decrease levels, (iii) inhibition 3-methyladenine or ATG5 led increase (iv) impairment,...
Primary Sjögren's syndrome (pSS) is a common chronic autoimmune disease characterized by lymphocytic infiltration of exocrine glands and peripheral lymphocyte perturbation. In the current study, we aimed to investigate possible pathogenic implication autophagy in T lymphocytes patients with pSS. Thirty consecutive pSS were recruited together 20 affected sicca and/or sialoadenitis 30 healthy controls. Disease activity damage evaluated according SS index, EULAR index. analyzed for expression...
We aimed at investigating whether the frequency and function of T helper 17 (Th17) regulatory cells (Treg) are affected by a restriction dietary sodium intake in patients with rheumatoid arthritis (RA) systemic lupus erythematosus (SLE). enrolled RA SLE not receiving drugs known to increase urinary excretion. Patients underwent regimen starting restricted daily followed normal-sodium intake. The timepoints were identified baseline (T0), after 3 weeks low-sodium (T3), 2 (T5). On these visits,...
Autophagy has emerged as a key mechanism in the survival and function of T B lymphocytes, its activation was involved apoptosis resistance rheumatoid arthritis (RA). To investigate whether relationship between autophagy may impact response to therapy, we analyzed ex vivo spontaneous patients with RA subjected treatment anti-tumor necrosis factor (TNF) drugs vitro effects TNFα anti-TNF on cell fate. Peripheral blood mononuclear cells (PBMCs) from 25 treated were for levels marker LC3-II by...
The safety and activity of the multikinase inhibitor sorafenib were investigated in patients with relapsed or refractory lymphoproliferative disorders who received (400 mg) twice daily until disease progression appearance significant clinical toxicity. primary endpoint was overall response rate (ORR). Biomarkers analysed at baseline during treatment. Thirty (median age, 61 years; range, 18-74) a median 4 months therapy. Grade 3-4 toxicities included hand/foot skin reactions (20%), infections...
Salivary gland epithelial cells (SGECs) are key cellular drivers in the pathogenesis of primary Sjögren's syndrome (SS); however, mechanisms sustaining SGEC activation SS remain unclear. We undertook this study to determine role autophagy survival and SGECs SS.Primary isolated from minor SGs patients with or sicca were evaluated by flow cytometry, immunoblotting, immunofluorescence assess (autophagic flux, light chain 3 IIB [LC3-IIB], p62, LC3-IIB+/lysosome-associated membrane protein 1...
Rheumatoid Arthritis (RA) is a chronic systemic autoimmune disease. Modifications of gut microbiota seem to be associated with the disease, but impact on therapies' outcome remains unclear. A role T cells in RA pathogenesis has been addressed, particularly Th17/Treg balance. Our study aimed evaluate early (ERA) patients compared control group, fecal composition, short-chain fatty acids concentrations, and levels circulating their own cytokines, before after 3 months standard treatment...
The pathway of Janus tyrosine kinases (JAKs) has a central role in the pathogenesis Rheumatoid Arthritis (RA) by regulating multiple immune functions and cytokine production. JAK inhibitor tofacitinib is effective RA patients not responding to methotrexate or TNF-inhibitors. Since hyperactive autophagy been associated with impaired apoptosis fibroblast-like synoviocytes (FLS), we aimed investigate modulating these cells. FLS isolated from biopsies were cultured presence inducer rapamycin...