A5047656323- Systemic Lupus Erythematosus Research
- Systemic Sclerosis and Related Diseases
- Autophagy in Disease and Therapy
- Rheumatoid Arthritis Research and Therapies
- Inflammasome and immune disorders
- Extracellular vesicles in disease
- Platelet Disorders and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Renal Diseases and Glomerulopathies
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Salivary Gland Disorders and Functions
- Salivary Gland Tumors Diagnosis and Treatment
- Autoimmune and Inflammatory Disorders Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Calcium signaling and nucleotide metabolism
- Osteoarthritis Treatment and Mechanisms
- Prostate Cancer Diagnosis and Treatment
- Reproductive System and Pregnancy
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Long-Term Effects of COVID-19
- Medical and Biological Ozone Research
- Kidney Stones and Urolithiasis Treatments
- Glycosylation and Glycoproteins Research
Sapienza University of Rome
2019-2025
Johns Hopkins University
2022-2025
Johns Hopkins Medicine
2022-2024
San Salvatore Hospital
2023
Oklahoma Medical Research Foundation
2023
University of Oklahoma
2023
Universidad de Sevilla
2023
Asklepios Klinik Sankt Augustin
2023
University of Cologne
2023
Policlinico Umberto I
2019-2021
Lupus nephritis (LN) is a pathologically heterogenous autoimmune disease linked to end-stage kidney and mortality. Better therapeutic strategies are needed as only 30%-40% of patients completely respond treatment. Noninvasive biomarkers intrarenal inflammation may guide more precise approaches. Because urine collects the byproducts inflammation, we studied proteomic profiles 225 with LN (573 samples) in longitudinal Accelerating Medicines Partnership RA/SLE cohort. Urinary...
Salivary gland epithelial cells (SGECs) are key cellular drivers in the pathogenesis of primary Sjögren's syndrome (SS); however, mechanisms sustaining SGEC activation SS remain unclear. We undertook this study to determine role autophagy survival and SGECs SS.Primary isolated from minor SGs patients with or sicca were evaluated by flow cytometry, immunoblotting, immunofluorescence assess (autophagic flux, light chain 3 IIB [LC3-IIB], p62, LC3-IIB+/lysosome-associated membrane protein 1...
The pathway of Janus tyrosine kinases (JAKs) has a central role in the pathogenesis Rheumatoid Arthritis (RA) by regulating multiple immune functions and cytokine production. JAK inhibitor tofacitinib is effective RA patients not responding to methotrexate or TNF-inhibitors. Since hyperactive autophagy been associated with impaired apoptosis fibroblast-like synoviocytes (FLS), we aimed investigate modulating these cells. FLS isolated from biopsies were cultured presence inducer rapamycin...
Mitochondria are a source of autoantigens and damage-associated molecular patterns (DAMPs) in systemic lupus erythematosus (SLE). Nucleoids carrying TFAM (transcription factor A, mitochondrial) mitochondrial DNA (mtDNA) important DAMPs SLE. While mtDNA has been associated with anti-double-stranded (ds)DNA antibodies type I interferon (IFN-I), the immunogenic role SLE pathogenesis is unknown. Here, we characterised clinical transcriptional phenotypes linked to anti-TFAM Anti-TFAM were...
O059 / #244 Topic: AS16 - Lupus Nephritis-Pathogenesis ABSTRACT CONCURRENT SESSION 10: INTEGRATING PROTEOMIC & TRANSCRIPTOMICS IN SLE 24-05-2025 10:40 AM 11:40 Background/Purpose Treatment response in lupus nephritis (LN) remain inadequately low, highlighting the need for better understanding of LN pathogenesis to improve management. Single-cell transcriptomic studies are providing an unprecedented catalog cell states LN, yet their spatial organization is not well understood. Since...
Rheumatoid arthritis (RA) is a chronic autoimmune disease and rheumatoid factor (RF) anti-citrullinated protein antibodies (ACPA) are the most frequently detected autoantibodies (autoAbs). To date, more than 20% of RA cases still defined as seronegative forms (seronegative RA, SN-RA). The aim this study was to identify new antigenic targets autoAbs in patients, which can also be recognized SN-RA. Using proteomic approach, we tested sera from SN-RA patients by analyzing synovial fluid (SF)...
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the novel coronavirus, causing coronavirus disease 2019 (COVID-19). During virus infection, several pro-inflammatory cytokines are produced, leading to “cytokine storm.” Among these, interleukin (IL)-6, tumor necrosis factor‐α (TNF‐α), and IL-1β seem have a central role in progression exacerbation of disease, recruitment immune cells infection sites. Autophagy an evolutionarily conserved lysosomal degradation pathway involved...
Autophagy is a homeostatic process responsible for the self-digestion of intracellular components and antimicrobial defense by inducing degradation pathogens into autophagolysosomes. Recent findings suggest an involvement this in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, role autophagy immunological mechanisms disease 2019 (COVID-19) pathogenesis remains largely unexplored. This study reveals presence defects peripheral immune cells from COVID-19...
The origin and mechanisms of autoantigen generation in systemic lupus erythematosus (SLE) are poorly understood. Here, we identified SLE neutrophils activated vivo by IFN as a prominent source Ro52, also known tripartite motif-containing protein 21 (TRIM21), critical historically thought to be primarily generated keratinocytes SLE. Different from mononuclear cells keratinocytes, enriched several unique Ro52 species containing core sequence encoded exon 4 (Ro52Ex4) TRIM21. Ro52Ex4 is the main...
To investigate the expression of citrullinated and carbamylated proteins in extracellular microvesicles (EMVs) from RA patients.We enrolled 24 naïve for biological therapy 20 healthy donors (HD), matched age sex. For each patient, laboratory clinical data were recorded indexes measured (Clinical Disease Activity Index, Simplified DAS28). EMVs patients HD purified plasma by nanoparticle tracking analysis (NanoSight). Further, incubated with anti-citrullinated/carbamylated antibodies processed...
<h3>Background</h3> Despite optimal treatment, lupus nephritis (LN) remains associated with irreversible kidney damage1. A better understanding of the mechanisms underlying LN pathogenesis is needed to develop treatment targets. As part Accelerating Medicines Partnership (AMP), we discovered that urinary PR3, a neutrophil degranulation product, correlated histological activity implicating neutrophil/monocyte in proliferative LN, most aggressive type2. PR3 serine protease can mediate damage....
Thrombocytopenia is a manifestation associated with primary antiphospholipid syndrome (PAPS), and many studies have stressed the leading role played by platelets in pathogenesis of (APS). Platelets are highly specialized cells, their activation involves series rapid rearrangements actin cytoskeleton. Recently, we described presence autoantibodies against D4GDI (Rho GDP dissociation inhibitor beta, ARHGDIB) serum large subset SLE patients, observed that anti-D4GDI antibodies activated...
<h3>Background</h3> We employed urine proteomics to define the molecular signatures associated with histological features quantified by NIH activity and chronicity indices. <h3>Objectives</h3> <h3>Methods</h3> Glomerular interstitial lesions in lupus nephritis were (scored 0-3) based on revised 2018 International Society of Nephrology/Renal Pathology (ISN/RPS) classification for modified scoring system a central renal pathologist (JH). Urinary proteins (1200 biomarkers, RayBiotech Kiloplex)...
<h3>Background:</h3> Undifferentiated connective tissue disease (UCTD) is a condition characterized by serological evidence of autoimmunity and occurrence clinical symptoms suggestive systemic autoimmune diseases, yet not fulfilling specific classification/diagnostic criteria. From an evolutionary point view, three possible situations can be observed: (1) the so-called UCTD could represent early stage disease, such as Systemic Lupus Erythematosus (SLE); (2) remain stable without progressing...
ABSTRACT Background Interstitial fibrosis in lupus nephritis (LN) is often infiltrated by immune cells but typically regarded as nonspecific “scar reaction.” This study aimed to investigate the relationship between inflammatory and kidney disease progression LN. Methods tubular atrophy (IFTA) were scored 124 LN biopsies. Inflammation areas of IFTA (i-IFTA) was graded 0-3 according Banff Classification Allograft Pathology. Significant glomerular filtration rate (GFR) loss defined a decline...