A5052424690- Extracellular vesicles in disease
- Autophagy in Disease and Therapy
- Systemic Sclerosis and Related Diseases
- Osteoarthritis Treatment and Mechanisms
- Rheumatoid Arthritis Research and Therapies
- Systemic Lupus Erythematosus Research
- Immunodeficiency and Autoimmune Disorders
- Drug-Induced Adverse Reactions
- Autoimmune and Inflammatory Disorders Research
- Hematological disorders and diagnostics
- Platelet Disorders and Treatments
- Calcium signaling and nucleotide metabolism
- Biosimilars and Bioanalytical Methods
- Long-Term Effects of COVID-19
- Musculoskeletal synovial abnormalities and treatments
- Mesenchymal stem cell research
- Otitis Media and Relapsing Polychondritis
- Autoimmune and Inflammatory Disorders
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
Sapienza University of Rome
2020-2025
Policlinico Umberto I
2021
The pathway of Janus tyrosine kinases (JAKs) has a central role in the pathogenesis Rheumatoid Arthritis (RA) by regulating multiple immune functions and cytokine production. JAK inhibitor tofacitinib is effective RA patients not responding to methotrexate or TNF-inhibitors. Since hyperactive autophagy been associated with impaired apoptosis fibroblast-like synoviocytes (FLS), we aimed investigate modulating these cells. FLS isolated from biopsies were cultured presence inducer rapamycin...
Rheumatoid Arthritis (RA) is a systemic autoimmune disease characterized by chronic synovial inflammation affecting diarthrodial joints, with cartilage destruction and bone erosion. Environmental inflammatory stimuli can induce maturation of dendritic cells (DCs), which promote differentiation activation effector T lymphocytes. We previously highlighted the role extracellular microvesicles (EMVs) in pathogenesis carrying antigens that trigger autoantibody production. In this investigation we...
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the novel coronavirus, causing coronavirus disease 2019 (COVID-19). During virus infection, several pro-inflammatory cytokines are produced, leading to “cytokine storm.” Among these, interleukin (IL)-6, tumor necrosis factor‐α (TNF‐α), and IL-1β seem have a central role in progression exacerbation of disease, recruitment immune cells infection sites. Autophagy an evolutionarily conserved lysosomal degradation pathway involved...
Autophagy is a homeostatic process responsible for the self-digestion of intracellular components and antimicrobial defense by inducing degradation pathogens into autophagolysosomes. Recent findings suggest an involvement this in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, role autophagy immunological mechanisms disease 2019 (COVID-19) pathogenesis remains largely unexplored. This study reveals presence defects peripheral immune cells from COVID-19...
To investigate the expression of citrullinated and carbamylated proteins in extracellular microvesicles (EMVs) from RA patients.We enrolled 24 naïve for biological therapy 20 healthy donors (HD), matched age sex. For each patient, laboratory clinical data were recorded indexes measured (Clinical Disease Activity Index, Simplified DAS28). EMVs patients HD purified plasma by nanoparticle tracking analysis (NanoSight). Further, incubated with anti-citrullinated/carbamylated antibodies processed...
<h3>Background:</h3> Glucocorticoids (GCs) have been a cornerstone of the management rheumatoid arthritis (RA) since early 1950s. For long time GCs one few available therapeutic options with brilliant clinical result. In addition, due to their rapid effect, an appealing profile for treating flares or as "bridging" therapy in RA. However, despite benefits, they equally well-known adverse effects and it is generally accepted that long-term use GCs, particularly at higher doses, not advisable....
Aseptic abscesses syndrome is a rare but increasingly recognized disease that falls within the spectrum of autoinflammatory disorders. Here, we describe case patient who presented with abdominal pain and fever, along multiple extra-abdominal abscesses, in absence underlying hematologic, autoimmune, infectious, or neoplastic conditions. Initially, responded to glucocorticoids, experienced several flares upon discontinuation, leading initiation treatment TNFα inhibitor. After 5 years, an...
<h3>Background</h3> Osteoarthritis (OA) is a common chronic degenerative disease caused by progressive cartilage degradation [1]. In OA, regenerative medicine based on mesenchymal stem cells (MSCs) considered the most promising method for restoring articular [2]. However, high heterogeneity of MSCs together with invasive and painful procedures cell isolation represent main challenges tissue engineering technology (3)[4]. this regard, homogeneous population MSCs, chondroprogenitor (CPs), have...
<h3>Background</h3> Platelet factor 4 (PF4) is a protein with pro-clotting function expressed by activated platelets high affinity for anionic glycosaminoglycans present on the platelet surface. It has been shown that positively charged surface of PF4 tetramer interacts negatively regions β2glycoprotein I (β2GPI) domains, stabilising link between antigen and phospholipid surface, thus increasing possibility binding respective antibodies. In particular, selectively binds two molecules β2GPI,...
Background Microparticles (MPs) are fragments of surface membranes activated eukaryotic cells. They characterized by different dimensions (from 0.1 to 1μm) and expression antigens, depending on their origin. MPs important mediators cell-to-cell communication as they can be internalized in a dose-dependent manner macrophages, endothelial cells other cell types, influencing both functional phenotypic characteristics the target Even if formation is enhanced activation or apoptosis, constitutive...
Background: Joint involvement represents one of the most frequent features in patients affected by Systemic Lupus Erythematosus (SLE). This manifestation is characterized a great heterogeneity phenotype and severity: application more sensitive imaging techniques identified an erosive damage about 25% (1). has been associated with autoantibodies, such as anti-citrullinated (ACPA) anti-carbamylated proteins (antiCarP), previously Rheumatoid Arthritis (RA) patients. Recently, homocysteinylated...