A5010066743- Microbial Inactivation Methods
- HIV Research and Treatment
- Microfluidic and Bio-sensing Technologies
- Magnetic and Electromagnetic Effects
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Ion channel regulation and function
- RNA Interference and Gene Delivery
- Complement system in diseases
- Pulsed Power Technology Applications
- Immunotherapy and Immune Responses
- Listeria monocytogenes in Food Safety
- Plasma Applications and Diagnostics
- HIV/AIDS drug development and treatment
- Endoplasmic Reticulum Stress and Disease
- Toxin Mechanisms and Immunotoxins
- Cell Adhesion Molecules Research
- Bacteriophages and microbial interactions
- Lipid metabolism and biosynthesis
- Angiogenesis and VEGF in Cancer
- Autophagy in Disease and Therapy
- Axon Guidance and Neuronal Signaling
- Proteoglycans and glycosaminoglycans research
- Planarian Biology and Electrostimulation
- Cannabis and Cannabinoid Research
Old Dominion University
2016-2023
BioElectronics (United States)
2017
University of Turin
2010-2012
Istituto Superiore di Sanità
2001-2010
University of Miami
2009
Depending on the initiating stimulus, cancer cell death can be immunogenic or non-immunogenic. Inducers of (ICD) rely endoplasmic reticulum (ER) stress for trafficking danger signals such as calreticulin (CRT) and ATP. We found that nanosecond pulsed electric fields (nsPEF), an emerging new modality tumor ablation, cause activation ER-resident sensor PERK in both CT-26 colon carcinoma EL-4 lymphoma cells. correlates with sustained CRT exposure plasma membrane apoptosis induction...
Abstract Previous studies reported a delayed increase of sensitivity to electroporation (termed “electrosensitization”) in mammalian cells that had been subjected electroporation. Electrosensitization facilitated membrane permeabilization and reduced survival cell suspensions when the electric pulse treatments were split fractions. The present study was aimed visualize effect sensitization establish its utility for ablation. We used KLN 205 squamous carcinoma embedded an agarose gel...
Accumulating data indicates that some cancer treatments can restore anticancer immunosurveillance through the induction of tumor immunogenic cell death (ICD). Nanosecond pulsed electric fields (nsPEF) have been shown to efficiently ablate melanoma tumors. In this study we investigated mechanisms and immunogenicity nsPEF-induced in B16F10 Our show vitro nsPEF (20-200, 200-ns pulses, 7 kV/cm, 2 Hz) caused a rapid dose-dependent which was not accompanied by caspase activation or PARP cleavage....
CD151, a transmembrane protein of the tetraspanin family, is implicated in regulation cell-substrate adhesion and cell migration through physical functional interactions with integrin receptors. In contrast, little known about potential role CD151 controlling proliferation survival. We have previously shown that β4 integrin, major partner, not only acts as an adhesive receptor for laminins but also intracellular signaling platform promoting invasive growth upon interaction Met, tyrosine...
Nanosecond pulsed electric fields (nsPEF) are emerging as a novel modality for cell stimulation and tissue ablation. However, the downstream protein effectors responsible nsPEF bioeffects remain to be established. Here we demonstrate that activate TMEM16F (or Anoctamin 6), functioning Ca2+-dependent phospholipid scramblase Ca2+-activated chloride channel. Using confocal microscopy patch clamp recordings, investigated relevance of activation several triggered by nsPEF, including...
Abstract The presence of human immunodeficiency virus (HIV)‐infected macrophages in the parenchyma central nervous system is an hallmark acquired syndrome‐related neuroinflammation. Once penetrated blood–brain barrier (BBB), closely interact with astrocytes, beginning those lying beneath BBB endothelium. By investigating consequences cell–cell interaction between HIV‐infected and we observed that HIV‐1 expression macrophagic cells correlated increased chemotactic activity supernatants...
Human immunodeficiency virus type 1 (HIV-1)-infected cells transmit viral products to uninfected CD4(+) very rapidly. However, the natures of transmitted and mechanism transmission, as well relative virological consequences, have not yet been fully clarified. We studied events occurring a few hours after contact between HIV-1-infected using coculture cell system in which expression target could be monitored through induction green fluorescent protein reporter gene driven by HIV-1 long...
We demonstrate that conditioning of mammalian cells by electroporation with nanosecond pulsed electric field (nsPEF) facilitates their response to the next nsPEF treatment. The experiments were designed unambiguously separate electroporation-induced sensitization and desensitization effects. Electroporation was achieved bursts 300-ns, 9 kV/cm pulses (50 Hz, n = 3-100) quantified propidium dye uptake within 11 min after exposure. observed either or no change (when too weak intense, when wait...
It was previously reported that human immunodeficiency virus type 1 (HIV-1) spreads in CD4 lymphocytes through cell-to-cell transmission. Here we report HIV-1-infected macrophages, but not lymphocytes, transmit HIV-1 products to CD4-negative cells of either epithelial, neuronal, or endothelial origin the absence overt infection. This phenomenon detectable as early h after start cocultivation and depended on contact release viral particles from donor cells. Transfer occurred upon their...
Nanosecond pulsed electric fields are emerging as a new modality for tissue and tumor ablation. We previously reported that cells exposed to develop hypersensitivity subsequent field applications. This phenomenon, named electrosensitization, is evoked by splitting the treatment in fractions (split-dose treatments) causes vitro 2- 3-fold increase cytotoxicity. The aim of this study was show benefit split-dose treatments vivo ablation nanosecond field. KLN 205 squamous carcinoma were embedded...
Abstract Electroporation by nanosecond electric pulses (nsEP) is an emerging modality for tumor ablation. Here we show the efficient induction of apoptosis even a non-toxic nsEP exposure when it followed 30-min chilling on ice. This itself had no impact survival U-937 or HPAF-II cells, but caused more than 75% lethality in nsEP-treated cells (300 ns, 1.8-7 kV/cm, 50-700 pulses). The cell death was largely delayed 5-23 hr and accompanied 5-fold activation caspase 3/7 (compared to without...
Abstract Although electric field–induced cell membrane permeabilization (electroporation) is used in a wide range of clinical applications from cancer therapy to cardiac ablation, the cellular- and molecular-level details processes that determine success or failure these treatments are poorly understood. Nanosecond pulsed field (nsPEF)–based tumor therapies known have an immune component, but whether how cells sense electroporative damage respond it not been demonstrated. Damage-...
Abstract Background The availability of cell lines releasing fluorescent viral particles can significantly support a variety investigations, including the study virus-cell interaction and screening antiviral compounds. Regarding HIV-1, recovery such biologic reagents represents very hard challenge due to intrinsic cytotoxicity many HIV-1 products. We sought overcome limitation by using line in an inducible way, exploiting ability Nef mutant be incorporated virions at quite high levels....
In HIV-infected patients, DC are likely to interact with both cell-free HIV and cells. We were interested in investigating the mechanism of virus transmission occurring upon contact between HIV-1-infected cells DC, as well consequences for HIV-1 Ag-presenting activity. By comparing mixed co-cultures trans-well cultures, we observed that cell-to-cell strongly increased Env-mediated virion endocytosis target DC. This was independent tropism, de novo infection, Env-CD4-dependent fusion,...
The Gram-positive anaerobic bacterium Cutibacterium acnes (C. acnes) is a commensal of the human skin, but also an opportunistic pathogen that contributes to pathophysiology skin disease acne vulgaris. C. can form biofilms; cells in biofilms are more resilient antimicrobial stresses. Acne therapeutic options such as topical or systemic treatments often show incomplete responses. In this study we measured efficacy nanosecond pulsed electric fields (nsPEF), new promising cell and tissue...
Sexual transmission is now the most frequent means of diffusion human immunodeficiency virus type 1 (HIV-1). Even if underlying mechanism still largely unknown, there a consensus regarding key role played by mucosal dendritic cells (DCs) in capturing HIV through contact with infected subepithelial lymphocytes, and their capacity to spread trans -infection. We found that protease inhibitors (PIs) reduced virion endocytosis strongly monocyte-derived immature (i) DCs contacting HIV-1-infected...