A5043121417- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Colorectal Cancer Treatments and Studies
- interferon and immune responses
- Immune cells in cancer
- CAR-T cell therapy research
- Histone Deacetylase Inhibitors Research
- Cancer Research and Treatments
- 3D Printing in Biomedical Research
- Tuberculosis Research and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Chemokine receptors and signaling
- COVID-19 Clinical Research Studies
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Virus-based gene therapy research
- Advanced Biosensing Techniques and Applications
- vaccines and immunoinformatics approaches
- Cancer Cells and Metastasis
- Mycobacterium research and diagnosis
Istituto Superiore di Sanità
2016-2025
Molecular Oncology (United States)
2013-2016
British School at Rome
2013
AO Foundation
2013
Menlo School
2013
Uppsala University
2013
Hôpital Lyon Sud
2013
National Institutes of Health
1996-2006
National Institute of Allergy and Infectious Diseases
1996-2006
Center for Biologics Evaluation and Research
1997
Interferon (IFN) consensus sequence-binding protein (ICSBP) is a transcription factor playing critical role in the regulation of lineage commitment, especially myeloid cell differentiation. In this study, we have characterized phenotype and activation pattern subsets dendritic cells (DCs) ICSBP−/− mice. Remarkably, recently identified mouse IFN-producing (mIPCs) were absent all lymphoid organs from mice, as revealed by lack CD11clowB220+Ly6C+CD11b− cells. parallel, CD11c+ isolated spleens...
Successful chemotherapy accounts for both tumor-related factors and host immune response. Compelling evidence suggests that some chemotherapeutic agents can induce an immunogenic type of cell death stimulating tumor-specific immunity. Here, we show cyclophosphamide (CTX) exerts two types actions relevant the induction antitumor immunity in vivo: (i) effect on dendritic (DC) homeostasis, mediated by endogenous I interferons (IFN-I), leading to preferential expansion CD8α(+) DC, main subset...
Immunotherapy efficacy relies on the crosstalk within tumor microenvironment between cancer and dendritic cells (DCs) resulting in induction of a potent effective antitumor response. DCs have specific role recognizing cells, taking up antigens (Ags) then migrating to lymph nodes for Ag (cross)-presentation naïve T cells. Interferon-α-conditioned (IFN-DCs) exhibit marked phagocytic activity special ability inducing Ag-specific T-cell Here, we developed novel microfluidic platform recreating...
The reconstitution of a complex microenvironment on microfluidic chips is one the cornerstones to demonstrate improved flexibility these devices with respect macroscale in vitro approaches. In this work, we realised an on-chip model investigate interactions between cancer and immune system. To end, exploited mice deficient (Knock Out, KO) for interferon regulatory factor 8 (IRF-8), transcription essential induction competent responses, how IRF-8 gene expression contributes regulate melanoma...
The cancer microenvironment may be conceptually regarded as a pitch where the main players are resident and non-resident cellular components, each covering defined role interconnected by complex network of soluble mediators. crosstalk between these cells tumor within this environment crucially determines fate progression. Immune that infiltrate bed transported there blood circulation exert variety effects, either counteracting or favoring outgrowth. Here, we review discuss multiple...
Cross-presentation is a crucial mechanism for generating CD8 T cell responses against exogenous Ags, such as dead cell-derived Ag, and mainly fulfilled by CD8α(+) dendritic cells (DC). Apoptotic death occurring in steady-state conditions largely tolerogenic, thus hampering the onset of effector responses. Type I IFNs (IFN-I) have been shown to promote cross-priming soluble or viral partly through stimulation DC. By using UV-irradiated OVA-expressing mouse EG7 thymoma cells, we show that...
The first report on the antitumor effects of interferon α/β (IFN-I) in mice was published 50 years ago. IFN-α were immunotherapeutic drugs approved by FDA for clinical use cancer. However, their occurred at a time when most mechanisms action still unknown. These cytokines being used as either conventional cytostatic or non-specific biological response modifiers. Specific activities subsequently ascribed to IFN-I poorly considered use. Notably, lot data humans and underlines importance...
Nowadays, several types of tumors can benefit from the new frontier immunotherapy, due to recent increasing knowledge role immune system in cancer control. Among therapeutic strategies there is checkpoint blockade (ICB), able restore an efficacious antitumor immunity and significantly prolong overall survival (OS) patients with advanced such as melanoma non-small cell lung (NSCLC). Despite impressive efficacy these agents some patients, treatment failure resistance are frequently observed....
Abstract Background Inefficient T-cell access to the tumor microenvironment (TME) is among causes of immune-resistance. Previous evidence demonstrated that targeting CXCR4 improves anti-PD-1/PD-L1 efficacy reshaping TME. To evaluate role newly developed antagonists (PCT/IB2011/000120/ EP2528936B1/US2013/0079292A1) in potentiating anti-PD-1 two syngeneic murine models, MC38 colon cancer and B16 melanoma-human CXCR4-transduced, were employed. Methods Mice subcutaneously injected with (1 × 10 6...
A significant number of COVID-19 patients were shown to have neutralizing antibodies (NAB) against IFN; however, NAB specificity, fluctuation over time, associations with biochemical and hematological parameters, IFN gene expression are not well characterized. Binding (BAB) IFN-α/-β screened in patients' serum. All BAB positive sera, a subset respiratory samples, tested for IFN-α/-β/-ω, using an antiviral bioassay. Transcript levels IFN-α/-β/-ω IFN-stimulated genes (ISGs) quantified....
Mice with a null mutation of the gene encoding interferon consensus sequence-binding protein (ICSBP) develop chronic myelogenous leukemia-like syndrome and mount impaired responses to certain viral bacterial infections. To gain mechanistic understanding contributions ICSBP humoral cellular immunity, we characterized control ICSBP-/- mice infection influenza A (flu) Leishmania major (L. major). both genotypes survived infections flu, but differed markedly in isotype distribution antiflu...
Scope of the present work is to infer migratory ability leukocytes by stochastic processes in order distinguish spontaneous organization immune cells against an insult (namely cancer). For this purpose, spleen from immunodeficient mice, selectively lacking transcription factor IRF-8 (IRF-8 knockout; KO), or immunocompetent animals (wild-type; WT), were allowed interact, alternatively, with murine B16.F10 melanoma ad hoc microfluidic environment developed on a LabOnChip technology. In...
// Sara Santagata 1 , Maria Napolitano Crescenzo D'Alterio Sonia Desicato 2 Salvatore Di Maro 3 Luciana Marinelli Alessandra Fragale 4 Buoncervello Francesco Persico 5 Lucia Gabriele Ettore Novellino Nicola Longo Sandro Pignata Sisto Perdonà and Stefania Scala Functional Genomics, Istituto Nazionale per lo Studio e la Cura dei Tumori, Fondazione “G. Pascale”-IRCCS, 80131 Naples, Italy Uro-Gynecological Department, Department of Pharmacy, University Naples Federico II,...
Mice with a null mutation of the gene encoding interferon consensus sequence-binding protein (ICSBP) develop disease marked expansion granulocytes and macrophages that frequently progresses to fatal blast crisis, thus resembling human chronic myelogenous leukemia (CML). One important feature CML is decreased responsiveness myeloid cells apoptotic stimuli. Here we show from mice deficient in ICSBP exhibit reduced spontaneous apoptosis significant decrease sensitivity induced by DNA damage. In...
Histone acetylation is thought to have a role in transcription. To gain insight into the of histone retinoid-dependent transcription, we studied effects trichostatin A (TSA), specific inhibitor deacetylase, on P19 embryonal carcinoma cells. We show that coaddition TSA and retinoic acid (RA) markedly enhances neuronal differentiation these cells, although alone does not induce but causes extensive apoptosis. Consistent with cooperative effect RA, two agents synergistically enhanced...
Abstract Regulatory T (Treg) cells are critical in inducing and maintaining tolerance. Despite progress understanding the basis of immune tolerance, mechanisms molecules involved generation Treg remain poorly understood. IFN regulatory factor (IRF)-1 is a pleiotropic transcription implicated regulation various processes. In this study, we report that IRF-1 negatively regulates CD4+CD25+ cell development function by specifically repressing Foxp3 expression. IRF-1-deficient (IRF-1−/−) mice...