A5083416686- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Cell Image Analysis Techniques
- Scientific Computing and Data Management
- Immune cells in cancer
- Advanced Fluorescence Microscopy Techniques
- Molecular Junctions and Nanostructures
- Neurogenesis and neuroplasticity mechanisms
- Receptor Mechanisms and Signaling
- Chemokine receptors and signaling
- Electrochemical sensors and biosensors
- Research Data Management Practices
- Various Chemistry Research Topics
- Barrier Structure and Function Studies
- Genetics, Bioinformatics, and Biomedical Research
- Single-cell and spatial transcriptomics
Heinrich Heine University Düsseldorf
2023-2025
Université Grenoble Alpes
2022-2023
European Molecular Biology Laboratory
2018-2023
Grenoble Institute of Neurosciences
2022-2023
Abstract Bioimage data are generated in diverse research fields throughout the life and biomedical sciences. Its potential for advancing scientific progress via modern, data‐driven discovery approaches reaches beyond disciplinary borders. To fully exploit this potential, it is necessary to make bioimaging data, general, multidimensional microscopy images image series, FAIR, that is, findable, accessible, interoperable reusable. These FAIR principles management now widely accepted community...
The structure and function of the vertebrate retina have been extensively studied across species with an isolated, ex vivo preparation. Retinal in vivo, however, remains elusive, especially awake animals. Here, we performed single-unit extracellular recordings optic tract head-fixed mice to compare output awake, anesthetized, retinas. While visual response properties were overall similar conditions, found that retinal had general (1) faster kinetics less variability latencies; (2) a larger...
Retinotopy, like all long-range projections, can arise from the axons themselves or their targets. The underlying connectivity pattern, however, remains elusive at fine scale in mammalian brain. To address this question, we functionally mapped spatial organization of input and target neurons female mouse retinocollicular pathway single-cell resolution using vivo two-photon calcium imaging. We found a near-perfect retinotopic tiling retinal ganglion cell axon terminals, with an average error...
DATA REPORT article Front. Neuroanat., 05 December 2018 Volume 12 - | https://doi.org/10.3389/fnana.2018.00105
Abstract Retinotopy, like all long-range projections, can arise from the axons themselves or their targets. The underlying connectivity pattern, however, remains elusive at fine scale in mammalian brain. To address this question, we functionally mapped spatial organization of input and target neurons mouse retinocollicular pathway single-cell resolution using vivo two-photon calcium imaging. We found a near-perfect retinotopic tiling retinal ganglion cell axon terminals, with an average...
Abstract Modern bioimaging core facilities at research institutions are essential for managing and maintaining high-end instruments, providing training support researchers in experimental design, image acquisition data analysis. An important task these is the professional management of complex multi-dimensional data, which often produced large quantity very different file formats. This article details process that led to successfully implementing OME Remote Objects system (OMERO)...
Abstract Modern bioimaging core facilities at research institutions are essential for managing and maintaining high‐end instruments, providing training support researchers in experimental design, image acquisition data analysis. An important task these is the professional management of complex multidimensional data, which often produced large quantity very different file formats. This article details process that led to successfully implementing OME Remote Objects system (OMERO)...
Microglia are non-neuronal cells of the myeloid lineage that invade and take up long-term residence in brain during development (Ginhoux et al. 2010) increasingly implicated neuronal maturation, homeostasis, pathology (Bessis 2007; Paolicelli 2011; Li 2012; Aguzzi 2013, Cunningham 2013). Since early twentieth century several methods for staining visualizing microglia have been developed. Scientists Ramón y Cajal’s group (Achúcarro 1913, Río-Hortega 1919) pioneered these their work led to...
Abstract The structure and function of the vertebrate retina have been extensively studied across species with an isolated, ex vivo preparation. Retinal in , however, remains elusive, especially awake animals. Here we performed single-unit extracellular recordings optic tract head-fixed mice to compare output awake, anesthetized, retinas. While visual response properties were overall similar conditions, found that retinal had general 1) faster kinetics less variability latencies; 2) a larger...
This protocol describe step by how to download and explore the dataset provided article Three-dimensional nanostructure of an intact microglia cell Bolasco et al 2018.