A5067310662- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Phylogenetic Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Gene Regulatory Network Analysis
- 3D Printing in Biomedical Research
- Biofuel production and bioconversion
- Enzyme Structure and Function
- bioluminescence and chemiluminescence research
- Plant Disease Resistance and Genetics
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Escherichia coli research studies
- Microfluidic and Bio-sensing Technologies
- Probiotics and Fermented Foods
- Microfluidic and Capillary Electrophoresis Applications
- Fungal and yeast genetics research
- Glycosylation and Glycoproteins Research
- Microbial infections and disease research
- CRISPR and Genetic Engineering
- Legume Nitrogen Fixing Symbiosis
- Microbial Community Ecology and Physiology
- Food composition and properties
Forschungszentrum Jülich
2016-2025
Institut des Géosciences de l'Environnement
2019
ETH Zurich
2009-2016
Board of the Swiss Federal Institutes of Technology
2009
ABSTRACT The activity of bacteriophages and phage-related mobile elements is a major source for genome rearrangements genetic instability their bacterial hosts. the industrial amino acid producer Corynebacterium glutamicum ATCC 13032 contains three prophages (CGP1, CGP2, CGP3) so far unknown functionality. Several phage genes are regularly expressed, large prophage CGP3 (∼190 kbp) has recently been shown to be induced under certain stress conditions. Here, we present construction MB001,...
Corynebacterium glutamicum is a Gram-positive soil bacterium that prefers the simultaneous catabolism of different carbon sources rather than their sequential utilization. This type metabolism requires an adaptation utilization rates to overall metabolic capacity. Here we show how two functionally redundant GntR-type transcriptional regulators, designated GntR1 and GntR2, co-ordinately regulate gluconate glucose uptake. GntR2 strongly repress genes encoding permease (gntP), kinase (gntK),...
Bacteria have evolved regulatory traits to rapidly adapt changing conditions. Two principal mechanisms modulate gene expression consist of regulation via alternative sigma factors and phosphorylation-dependent response regulators. PhyR represents a recently discovered protein family combining parts both systems: factor-like domain the extracytoplasmic function (ECF) subfamily linked receiver regulator. Here we investigated mode action this key regulator general stress in Methylobacterium...
For synthetic biology applications, a robust structural basis is required, which can be constructed either from scratch or in top-down approach starting any existing organism. In this study, we initiated the construction of chassis organism Corynebacterium glutamicum ATCC 13032, aiming for relevant gene set to maintain its fast growth on defined medium. We evaluated each native essentiality considering expression levels, phylogenetic conservation, and knockout data. Based classification,...
The majority of biotechnologically relevant metabolites do not impart a conspicuous phenotype to the producing cell. Consequently, analysis microbial metabolite production is still dominated by bulk techniques, which may obscure significant variation at single-cell level. In this study, we have applied recently developed Lrp-biosensor for monitoring amino acid in single cells gradually engineered L-valine Corynebacterium glutamicum strains based on pyruvate dehydrogenase complex-deficient...
Abstract Cell‐to‐cell heterogeneity typically evolves due to a manifold of biological and environmental factors special phenotypes are often relevant for the fate whole population but challenging detect during conventional analysis. We demonstrate microfluidic single‐cell cultivation platform that incorporates several hundred growth chambers, in which isogenic bacteria microcolonies growing cell monolayers tracked by automated time‐lapse microscopy with spatiotemporal resolution. The device...
Predation by phages is a major driver of bacterial evolution. As result, elucidating antiphage strategies crucial from both fundamental and therapeutic standpoints.
PhyR is an unusual type of response regulator consisting a receiver domain and extracytoplasmic function (ECF) sigma factor-like domain. It was recently described as master general stress in Methylobacterium extorquens. Orthologues this are present essentially all free-living Alphaproteobacteria. In most them, phyR genetically closely linked to gene encoding ECF factor. Here, we investigate the role these two regulators soybean symbiont Bradyrhizobium japonicum USDA110. Using deletion...
Targeted top-down strategies for genome reduction are considered to have a high potential providing robust basic strains synthetic biology and industrial biotechnology. Recently, we created library of 26 genome-reduced Corynebacterium glutamicum carrying broad deletions in single gene clusters showing wild-type-like biological fitness. Here, proceeded with combinatorial these irrelevant two parallel orders, the resulting 28 was characterized under various environmental conditions. The final...
The fast-growing marine bacterium Vibrio natriegens represents an emerging model host for molecular biology and biotechnology, featuring a reported doubling time of less than 10 minutes. In many bacterial species, viral DNA (prophage elements) may constitute considerable fraction the whole genome have detrimental effects on growth fitness industrial strains. Genome analysis revealed presence two prophage regions in V. that were shown to undergo spontaneous induction under standard...
The genome of the Gram-positive soil bacterium Corynebacterium glutamicum ATCC 13032 contains three integrated prophage elements (CGP1 to -3). Recently, it was shown that large lysogenic CGP3 (∼187 kbp) is excised spontaneously in a small number cells. In this study, we provide evidence induced SOS response partly responsible for observed spontaneous induction. Whereas previous studies focused mainly on induction prophages at population level, analyzed single-cell level using promoters phage...
In this protocol the fabrication, experimental setup and basic operation of recently introduced microfluidic picoliter bioreactor (PLBR) is described in detail. The PLBR can be utilized for analysis single bacteria microcolonies to investigate biotechnological microbiological related questions concerning, e.g. cell growth, morphology, stress response, metabolite or protein production on single-cell level. device features continuous media flow enabling constant environmental conditions...
Abstract In this work, we performed a comparative adaptive laboratory evolution experiment of the important biotechnological platform strain Corynebacterium glutamicum ATCC 13032 and its prophage-free variant MB001 towards improved growth rates on glucose minimal medium. Both strains displayed comparable adaptation behavior no significant differences in genomic rearrangements mutation frequencies. Remarkably, fitness leap by about 20% was observed for both already after 100 generations....
The formation of plaques represents the hallmark phage infection visualizing clearance bacterial lawn in structured environments. In this study, we have addressed impact cellular development on Streptomyces undergoing a complex developmental life cycle. Analysis plaque dynamics revealed, after period size enlargement, significant regrowth transiently phage-resistant mycelium into lysis zone. venezuelae mutant strains defective at different stages indicated that was dependent onset aerial...
The response regulator HrrA of the HrrSA two-component system (previously named CgtSR11) was recently found to be repressed by global iron-dependent DtxR in Corynebacterium glutamicum. Here, we provide evidence that mediates heme-dependent gene regulation this nonpathogenic soil bacterium. Growth experiments and DNA microarray analysis revealed C. glutamicum is able use hemin as an alternative iron source emphasize involvement putative ABC transporter HmuTUV heme oxygenase (HmuO)...