A5035131482- Microbial Metabolic Engineering and Bioproduction
- Microbial Natural Products and Biosynthesis
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Fungal Biology and Applications
- Plant biochemistry and biosynthesis
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Bacteriophages and microbial interactions
- Biochemical and Molecular Research
- HIV Research and Treatment
- Transgenic Plants and Applications
- Epigenetics and DNA Methylation
- Microbial Metabolism and Applications
- Plant tissue culture and regeneration
- Enzyme Production and Characterization
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Microbial Metabolites in Food Biotechnology
Symrise (Germany)
2023-2024
Forschungszentrum Jülich
2018-2024
John Wiley & Sons (United States)
2019
Hudson Institute
2019
Predation by phages is a major driver of bacterial evolution. As result, elucidating antiphage strategies crucial from both fundamental and therapeutic standpoints.
Abstract Directed evolution of the O ‐methyltransferase ZgOMT from Zooshikella ganghwensis focusing on active site residues resulted in highly regioselective biocatalysts (regioisomeric ratios up to 99 : 1) for preparation taste hesperetin dihydrochalcone and related compounds. These newly constructed enzyme variants provide an attractive synthesis route para ‐methylation catechol scaffolds, which is challenging perform with high regioselectivity utilizing wild‐type ‐methyltransferases.
Abstract In recent years microorganisms have been engineered towards synthesizing interesting plant polyphenols such as flavonoids and stilbenes from glucose. Currently, the low endogenous supply of malonyl‐CoA, indispensable for polyphenol synthesis, impedes high product titers. Usually, limited malonyl‐CoA availability during production is avoided by supplementing fatty acid synthesis‐inhibiting antibiotics cerulenin, which are known to increase intracellular pool a side effect. Motivated...
In the last years, different biotechnologically relevant microorganisms have been engineered for synthesis of plant polyphenols such as flavonoids and stilbenes. However, low intracellular availability malonyl-CoA essential precursor most interest is regarded decisive bottleneck preventing high product titers. this study, Corynebacterium glutamicum, which emerged promising cell factory polyphenol production, was tailored by rational metabolic engineering towards providing significantly more...
Abstract Background The phenylbutanoid 4-(4-hydroxyphenyl)butan-2-one, commonly known as raspberry ketone, is responsible for the typical scent and flavor of ripe raspberries. Chemical production nature-identical ketone well established this compound frequently used to food, beverages perfumes. However, high demand natural but low abundance in raspberries, render one most expensive flavoring components. Results In study, Corynebacterium glutamicum was engineered microbial synthesis character...
Abstract Members of the bacterial phylum Planctomycetota have recently emerged as promising and for most part untapped sources novel bioactive compounds. The characterization more than 100 species in last decade stimulated recent bioprospection studies that start to unveil chemical repertoire phylum. In this study, we performed systematic bioinformatic analyses based on genomes all 131 described members current focusing identification type III polyketide synthase (PKS) genes. Type PKSs are...
Due to the increasing demand for natural food ingredients, including taste-active compounds, enzyme-catalyzed conversions of substrates, such as flavonoids, are promising tools align with principles Green Chemistry. In this study, a novel
Abstract In response to viral predation, bacteria have evolved a wide range of defense mechanisms, which rely mostly on proteins acting at the cellular level. Here, we show that aminoglycosides, well-known class antibiotics produced by Streptomyces , are potent inhibitors phage infection in widely divergent bacterial hosts. We demonstrate aminoglycosides block an early step life cycle, prior genome replication. Phage inhibition was also achieved using supernatants from natural aminoglycoside...
Abstract Type I polyketide synthases (PKSs) are large multi-domain proteins converting simple acyl-CoA thioesters such as acetyl-CoA and malonyl-CoA to a diversity of biotechnologically interesting molecules. Such multi-step reaction cascades particular interest for applications in engineered microbial cell factories, the introduction single protein with many enzymatic activities does not require balancing several individual activities. However, functional type PKSs into heterologous hosts...
A comparison of human mesenchymal stem cell osteogenesis in poly(ethylene glycol)