A5003739680- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Microbial metabolism and enzyme function
- biodegradable polymer synthesis and properties
- Metabolism and Genetic Disorders
- Adipose Tissue and Metabolism
- Microbial Natural Products and Biosynthesis
- Virus-based gene therapy research
- Biochemical Acid Research Studies
- Amino Acid Enzymes and Metabolism
- Algal biology and biofuel production
- Pancreatic function and diabetes
- Diet and metabolism studies
- Fungal and yeast genetics research
- Fungal Biology and Applications
- Enzyme Production and Characterization
- Metal-Catalyzed Oxygenation Mechanisms
- Microbial Inactivation Methods
- Gene Regulatory Network Analysis
Forschungszentrum Jülich
2015-2024
Stadtwerke Jülich (Germany)
2004
Overflow metabolism is well known for yeast, bacteria and mammalian cells. It typically occurs under glucose excess conditions characterized by excretions of by-products such as ethanol, acetate or lactate. This phenomenon, also denoted the short-term Crabtree effect, has been extensively studied over past few decades, however, its basic regulatory mechanism functional role in still unknown. Here we present a comprehensive quantitative time-dependent analysis exometabolome Escherichia coli,...
The functional properties of glycoproteins are strongly influenced by their profile glycosylation, and changes in this seen malignancy. In mucin-type O-linked glycosylation these can result the production mucins such as MUC1, carrying shorter sialylated O-glycans, with different site occupancy. Of tumor-associated disaccharide, sialyl-Tn (sialic acid α2,6GalNAc), is expressed 30% breast carcinomas most tumor-specific. ST6GalNAc-I glycosyltransferase, which catalyze transfer sialic to GalNAc,...
In a former study we showed that Corynebacterium glutamicum grows much faster in defined CGXII glucose medium when growth was initiated highly diluted environments [Grünberger et al. (2013b) Biotechnol Bioeng]. Here studied the batch of C. at comparable low starting biomass concentration OD ≈ 0.005 more detail. During bioreactor cultivations bi-phasic behavior with changing rates observed. Initially culture grew μˆ=0.61±0.02 h-1 before rate dropped to μˆ=0.46±0.02 h-1. We were able confirm...
A robust cell factory that can tolerate combined inhibitory lignocellulosic compounds is essential for the cost-effective lignocellulose-based production of second-generation bioethanol and other bulk chemicals. Following high-throughput phenotyping a yeast genomic overexpression library, we identified Saccharomyces cerevisiae mutant (denoted AFb.01) with improved growth fermentation performance under toxicity acetic acid furfural. AFb.01 carries TRX1, which encodes thioredoxin, cellular...
Bio-based bulk chemicals such as carboxylic acids continue to struggle compete with their fossil counterparts on an economic basis. One possibility improve the feasibility is use of crude substrates in biorefineries. However, impurities these pose challenges fermentation and purification, requiring interdisciplinary research. This work demonstrates a holistic approach biorefinery process development, using itaconic acid production thick juice based sugar beets Ustilago sp. example. A...
Abstract The acetic acid bacterium (AAB) Gluconobacter oxydans incompletely oxidizes a wide variety of carbohydrates and is therefore used industrially for oxidative biotransformations. For G. , no system was available that allows regulatable plasmid-based expression. We found the l -arabinose-inducible P BAD promoter transcriptional regulator AraC from Escherichia coli MC4100 performed very well in . respective pBBR1-based plasmids showed low basal expression reporters β-glucuronidase...
In this work, we established an efficient process for the production of itaconate from regionally sourced industrial side-stream molasses using Ustilago cynodontis and maydis. While being relatively cheap more environmentally friendly than refined sugars, there are some major challenges to overcome when working with molasses. Some those a high nitrogen load, unknown impurities in feedstock, amounts ill-favoured carbon sources, such as sucrose or lactate. We could show that activity...
Abstract Members of the bacterial phylum Planctomycetota have recently emerged as promising and for most part untapped sources novel bioactive compounds. The characterization more than 100 species in last decade stimulated recent bioprospection studies that start to unveil chemical repertoire phylum. In this study, we performed systematic bioinformatic analyses based on genomes all 131 described members current focusing identification type III polyketide synthase (PKS) genes. Type PKSs are...
Short-chain fatty acids (SCFAs) are bacterial products that known to be used as energy sources in eukaryotic hosts, whereas their role the metabolism of intestinal microbes is rarely explored. In present study, acetic, propionic, butyric, isobutyric, valeric, and isovaleric acid, respectively, were added a newly defined medium containing Prevotella bryantii B14 cells. After 8 h 24 h, optical density, pH SCFA concentrations measured. Long-chain acid (LCFA) profiles cells analyzed via gas...
In recent years branched short-chain dicarboxylates (BSCD) such as itaconic acid gained increasing interest in both medicine and biotechnology. Their use building blocks for plastics urges developing microbial upcycling strategies to provide sustainable end-of-life solutions. Furthermore, many BSCD exhibit anti-bacterial properties or exert immunomodulatory effects macrophages, indicating a medical relevance this group of molecules. For these applications, detailed understanding the...
Abstract Impranil ® DLN‐SD is a poly(ester‐urethane) (PEU) that widely used as coating material for textiles to fine‐tune and improve their properties. Since coatings increase the complexity of such plastic materials, they can pose hindrance sustainable end‐of‐life solutions plastics using enzymes or microorganisms. In this study, we isolated Halopseudomonas formosensis FZJ due its ability grow on other PEUs sole carbon sources. The strain was exceptionally thermotolerant it could degrade at...
3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic compound naturally found in edible vegetables and medicinal herbs. PCA of high interest the chemical industry has wide potential for pharmaceutical applications. We designed constructed novel Corynebacterium glutamicum strain to enable efficient utilization d-xylose microbial production PCA. Shake flask cultivation engineered showed maximum titer 62.1 ± 12.1 mM (9.6 1.9 g L-1 ) from as primary carbon energy source. The corresponding...
Ruminants such as cattle and sheep depend on the breakdown of carbohydrates from plant-based feedstuff, which is accomplished by microbial community in rumen. Roughly 40% members rumen microbiota belong to family Prevotellaceae, ferments sugars organic acids acetate, propionate, succinate. These substrates are important nutrients for ruminant. In a metaproteome analysis cattle, proteins that homologous Na+-translocating NADH:quinone oxidoreductase (NQR) quinone:fumarate reductase (QFR) were...
The economically efficient utilization of NADPH or NADH-dependent enzymes requires the regeneration consumed reduction equivalents. This cofactor is classically done via an additional substrate, and if necessary enzyme. We now demonstrate easy-to-apply approach, which can especially be used in screening applications. Simply by applying citrate to a buffer directly using citrate/-phosphate regenerated native TCA cycle, practically present all aerobic living organisms. Apart from...
Fe(II)/α-ketoglutarate-dependent dioxygenases (KDOs) catalyze a broad range of selective C–H oxidation reactions. However, the difficult production KDOs in recombinant E. coli strains and their instability purified form have so far limited application preparative biotransformations. Here, we investigated immobilization three (CaKDO, CpKDO, FjKDO) that stereoselective hydroxylation L-lysine side chain using two one-step techniques (HaloTag®, EziG™). The HaloTag®-based immobilisates reached...
The halophilic archaeon Haloferax volcanii contains three operons encoding 2-oxoacid dehydrogenase complexes (OADHCs) OADHC1-OADHC3. However, the biological role of these OADHCs is not known as previous studies have demonstrated that they cannot use any OADHC substrates. Even construction single mutants in all oadhc operons, reported recently, could identify a substrate. Therefore, possible double and triple mutant were generated, single, compared to wild-type. four devoid functional OADHC1...