A5046548445- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Nitric Oxide and Endothelin Effects
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Mitochondrial Function and Pathology
- 3D Printing in Biomedical Research
- Criminal Law and Policy
- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolic Engineering and Bioproduction
- Immunotherapy and Immune Responses
- Eicosanoids and Hypertension Pharmacology
- Blood properties and coagulation
- CRISPR and Genetic Engineering
- Receptor Mechanisms and Signaling
- Advanced Proteomics Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Adenosine and Purinergic Signaling
- Carbohydrate Chemistry and Synthesis
- RNA Interference and Gene Delivery
- Adipose Tissue and Metabolism
- Angiogenesis and VEGF in Cancer
- Cardiac Ischemia and Reperfusion
- Healthcare Decision-Making and Restraints
TU Dresden
2012-2025
Bielefeld University
2015-2024
University Hospital Carl Gustav Carus
2012-2021
University of Zurich
2020
Carl Gustav Carus-Institut
2016
Justus-Liebig-Universität Gießen
2004-2014
GTx (United States)
2012
Universitätsklinikum Gießen und Marburg
2010
Czech Academy of Sciences, Institute of Physiology
2003-2009
Giessen School of Theology
2009
The functional properties of glycoproteins are strongly influenced by their profile glycosylation, and changes in this seen malignancy. In mucin-type O-linked glycosylation these can result the production mucins such as MUC1, carrying shorter sialylated O-glycans, with different site occupancy. Of tumor-associated disaccharide, sialyl-Tn (sialic acid α2,6GalNAc), is expressed 30% breast carcinomas most tumor-specific. ST6GalNAc-I glycosyltransferase, which catalyze transfer sialic to GalNAc,...
Somatic neural and crest stem cells are promising sources for cellular therapy of several neurodegenerative diseases. However, because practical considerations such as inadequate accessibility the source material, application is strictly limited. The secondary palate a highly regenerative heavily innervated tissue, which develops embryonically under direct contribution cells. Here, we describe first time presence nestin-positive crest-related within Meissner corpuscles Merkel cell-neurite...
The response of endothelial energy metabolism to oxygen supply was studied in cultured coronary cells from the rat at defined PO2 levels between 0.1 and 100 Torr. In presence glucose (5 mM), respiration (4 nmol O2.min-1.mg protein-1) independent exterior greater than 3 Torr; consumption half maximal 0.8 At Torr, lactate production 26 nmol.min-1.mg protein-1; decrease Torr resulted a 2.2-fold increase production. contents ATP, ADP, AMP were 21, 4, 2 nmol/mg protein, respectively; they...
For the improved production of vaccines and therapeutic proteins, a detailed understanding metabolic dynamics during batch or fed-batch is requested. To study new human cell line AGE1.HN, flexible flux analysis method was developed that considering dynamic changes in growth metabolism cultivation. This comprises formation cellular components as well conversion major substrates products, spline fitting data estimation using metabolite balancing. During cultivation AGE1.HN three distinct...
Abstract The tumour microenvironment plays a crucial role in the growth and progression of cancer, presence tumour-associated macrophages (TAMs) is associated with poor prognosis. Recent studies have demonstrated that TAMs display transcriptomic, phenotypic, functional geographical diversity. Here we show sialylated glycoform mucin MUC1, MUC1-ST, through engagement Siglec-9 can specifically independently induce differentiation monocytes into unique phenotype to best our knowledge has not...
Abstract Tumors exploit several strategies to evade immune recognition, including the production of a large number immunosuppressive factors, which leads reduced numbers and impaired functions dendritic cells (DCs) in vicinity tumors. We have investigated whether mucin released by tumor could be involved causing these immunomodulating effects on DCs. used recombinant purified form MUC1 glycoprotein, an epithelial associated that is overexpressed, aberrantly glycosylated, shed during cancer...
Chinese hamster ovary (CHO) cell lines represent the most commonly used mammalian expression system for production of therapeutic proteins. In this context, detailed knowledge CHO transcriptome might help to improve biotechnological processes conducted by specific lines. Nevertheless, very few assembled cDNA sequences cells were publicly released until recently, which puts a severe limitation on research. Two extended annotation systems and web-based tools, one browsing eukaryotic genomes...
Brown adipose tissue (BAT) thermogenesis dissipates energy through heat production and thereby it opposes metabolic disease. It is mediated by mitochondrial membrane uncoupling, yet the mechanisms sustaining potential (ΔΨm) in brown adipocytes are poorly understood. Here we show that isocitrate dehydrogenase (IDH) activity expression of soluble adenylate cyclase 10 (ADCY10), a CO2/bicarbonate sensor residing mitochondria, upregulated BAT cold-exposed mice. IDH inhibition or ADCY10 deficiency...
Ceftazidime (CAZ) resistance in Burkholderia pseudomallei , the causative agent of melioidosis, complicates treatment endemic regions. This study identified a novel A172T mutation and other known penA mutations as critical contributors to CAZ large Thai strain collection. Frequent gene duplication amplification (GDA) penA, likely driven by Palindromic GC-Rich Repeat Sequences (PGCRRS), highlights urgent need for rapid diagnostics optimized strategies manage this life-threatening disease effectively.
We have developed an expression system for the production of large quantities recombinant MUC1 mucin in CHO-K1 (Chinese-hamster ovary K1) cells. The extracellular part human MUC1, including 16 tandem repeats, was produced as a fusion protein with murine IgG Fc, intervening enterokinase cleavage site removal Fc tail. Stable MUC1–IgG-producing clones were generated and found to secrete MUC1–IgG into culture medium. After adaptation suspension protein-free medium bioreactor, secreted (100 mg/l...
AimsPhosphorylation of forkhead box O (FoxO) transcription factors induces their nuclear exclusion and proteosomal degradation. Here, we investigated the effect fluid shear stress on FoxO1a in primary cultures human endothelial cells kinases that regulate its phosphorylation.
Activation of cAMP signalling abrogates thrombin-induced hyperpermeability. One the mechanisms underlying this protective effect is inactivation endothelial contractile machinery, one major determinants barrier function, mainly via activation myosin light chain phosphatase (MLCP). To date, cAMP-mediated MLCP are only partially understood. Here contribution two effectors, PKA and Epac, in regulation machinery function was studied. Endothelial were analysed cultured human umbilical vein cells...
Accumulating evidence suggests a pivotal role of the calcitonin receptor-like receptor (CRLR) signaling pathway in preventing damage lung by stabilizing pulmonary barrier function. Intermedin (IMD), also termed adrenomedullin-2, is most recently identified peptide targeting this receptor. Here we investigated effect hypoxia on expression IMD murine and cultured microvascular endothelial cells (PMEC) as well regulating vascular permeability. Monoclonal antibodies were generated, transcript...