A5049883349- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Adipokines, Inflammation, and Metabolic Diseases
- Circular RNAs in diseases
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Cardiovascular Disease and Adiposity
- Mitochondrial Function and Pathology
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Regulation of Appetite and Obesity
- Complement system in diseases
- Lipid metabolism and disorders
- Liver Disease Diagnosis and Treatment
- Biochemical Analysis and Sensing Techniques
- Cardiac tumors and thrombi
- Medical Imaging and Pathology Studies
- Neurogenesis and neuroplasticity mechanisms
- Venous Thromboembolism Diagnosis and Management
- RNA modifications and cancer
- Metabolism, Diabetes, and Cancer
- Immune cells in cancer
- Appendicitis Diagnosis and Management
- Atrial Fibrillation Management and Outcomes
TU Dresden
2013-2025
University Hospital Carl Gustav Carus
2015-2025
Joslin Diabetes Center
2017-2024
Harvard University
2017-2024
Centro Nacional de Biotecnología
2023-2024
Universidad Autónoma de Madrid
2023-2024
Universidad de Málaga
2023
Universidad de Valladolid
2023
Hospital San Pedro
2020-2021
Hospital Universitario Reina Sofía
2016-2018
Exosomes/small extracellular vesicles (sEVs) can serve as multifactorial mediators of cell-to-cell communication through their miRNA and protein cargo. Quantitative proteomic analysis five cell lines representing metabolically important tissues reveals that each type has a unique sEV proteome. While classical markers such CD9/CD63/CD81 vary markedly in abundance, we identify six (ENO1, GPI, HSPA5, YWHAB, CSF1R, CNTN1) are similarly abundant sEVs all types. In addition, specific markers....
Significance Inflammation is a critical contributor to the pathogenesis of metabolic disorders associated with obesity. A group molecules crucial in regulating immune system are costimulatory molecules, including CD40. Our current study shows that CD40 acts as double-edged sword syndrome through initiation differential signaling cascades. The CD40-TNF receptor-associated factor (TRAF) 2/3/5 pathway protects against dysfunction and inflammation obesity; conversely, CD40-TRAF6 contributes...
Abstract Obese adipose tissue (AT) inflammation contributes critically to development of insulin resistance. The complement anaphylatoxin C5a receptor (C5aR) has been implicated in inflammatory processes and as regulator macrophage activation polarization. However, the role C5aR obesity AT not addressed. We engaged model diet-induced found that expression was significantly upregulated obese AT, compared with lean AT. In addition, present proximity macrophage-rich crownlike structures....
Local relapse and peritoneal carcinomatosis (PC) for pT4 colon cancer is estimated in 15,6% 36,7% 12 months 36 from surgical resection respectively, achieving a 5 years overall survival of 6%. There are promising results using prophylactic HIPEC this group patients, it that up to 26% all T4 could benefit treatment with minimal morbidity. Adjuvant effective avoid the possibility seeding after resection. Taking into account these cumulative experience use, we will lead randomized controlled...
Regulation of gene expression is an important aspect insulin action but in vivo intertwined with changing levels glucose and counter-regulatory hormones. Here we demonstrate that under euglycemic clamp conditions, physiological regulate interrelated networks more than 1,000 transcripts muscle liver. These include expected pathways related to lipid utilization, mitochondrial function, autophagy, as well unexpected pathways, such chromatin remodeling, mRNA splicing, Notch signaling. acutely...
Muscle regeneration is a complex process relying on precise teamwork between multiple cell types, including muscle stem cells (MuSCs) and fibroadipogenic progenitors (FAPs). FAPs are also the main source of intramuscular adipose tissue (IMAT). Muscles without exhibit decreased IMAT infiltration but deficient regeneration, indicating importance in repair process. Here, we demonstrate presence bidirectional crosstalk MuSCs via their secretion extracellular vesicles (EVs) containing distinct...
Pheochromocytomas and paragangliomas (PPGLs) are catecholamine‐producing chromaffin cell tumors with diverse phenotypic features reflecting mutations in numerous genes, including MYC‐associated factor X ( MAX ). To explore whether differences among PPGLs reflect a MAX‐mediated mechanism opposing influences of hypoxia‐inducible (HIF)s HIF2α HIF1α, we combined observational investigations gene‐manipulation studies two pheochromocytoma lines. Among from 140 patients, due to were characterized...
Angiogenesis is a central regulator for white (WAT) and brown (BAT) adipose tissue adaptation in the course of obesity. Here we show that deletion hypoxia-inducible factor 2α (HIF2α) adipocytes (by using Fabp4-Cre transgenic mice) but not myeloid or endothelial cells negatively impacted WAT angiogenesis promoted inflammation, dysfunction, hepatosteatosis, systemic insulin resistance Importantly, adipocyte HIF2α regulated vascular growth (VEGF) expression obese BAT as well its thermogenic...
The low-grade inflammatory state present in obesity contributes to obesity-related metabolic dysregulation, including nonalcoholic steatohepatitis (NASH) and insulin resistance. Intercellular interactions between immune cells or hepatic parenchymal contribute the exacerbation of liver inflammation steatosis obesity. costimulatory molecules, B7.1 B7.2, are important regulators cell-cell several processes; however, role B7 costimulation is unknown. Here, diet-induced (DIO) studies mice with...
Senescence in pancreatic beta cells plays a major role cell dysfunction, which leads to impaired glucose homeostasis and diabetes. Therefore, prevention of senescence could reduce the risk Treatment nonobese diabetic (NOD) mice, model type 1 autoimmune diabetes (T1D), with palmitic acid hydroxy stearic acids (PAHSAs), novel class endogenous lipids antidiabetic antiinflammatory effects, delays onset reduces incidence T1D from 82% vehicle treatment 35% PAHSAs. Here, we show that mechanism by...
Adipose tissue is no longer considered a passive energy store. Instead, it well established as an important endocrine contributor. Here, the authors comprehensively show that function of adipose organ not limited to classical adipokines, but also includes miRNAs and maybe other non-coding RNAs. This study indicates main source circulating miRNAs, only in mice humans. They dissected role different depots showed derived operate manner distant tissues, such liver. In summary, for first time...
Tissues release microRNAs (miRNAs) in small extracellular vesicles (sEVs) including exosomes, which can regulate gene expression distal cells, thus acting as modulators of local and systemic metabolism. Here, we show that insulin regulates miRNA secretion into sEVs from 3T3-L1 adipocytes this process is differentially regulated cellular expression. Thus, the 53 miRNAs upregulated 66 downregulated by sEVs, only 12 were parallel cells. Insulin part phosphorylating hnRNPA1, causing it to bind...
Brown adipose tissue (BAT) thermogenesis dissipates energy through heat production and thereby it opposes metabolic disease. It is mediated by mitochondrial membrane uncoupling, yet the mechanisms sustaining potential (ΔΨm) in brown adipocytes are poorly understood. Here we show that isocitrate dehydrogenase (IDH) activity expression of soluble adenylate cyclase 10 (ADCY10), a CO2/bicarbonate sensor residing mitochondria, upregulated BAT cold-exposed mice. IDH inhibition or ADCY10 deficiency...
The neural crest-derived adrenal medulla is closely related to the sympathetic nervous system; however, unlike tissue, it characterized by high plasticity which suggests involvement of stem cells. Here, we show that a defined pool glia-like nestin-expressing progenitor cells in adult contributes this plasticity. These have features adrenomedullary sustentacular cells, are multipotent, and able differentiate into chromaffin neurons. central body's response stress making its proper adaptation...
The brain is now recognized as an insulin-sensitive tissue; however, the role of changing insulin concentrations in peripheral circulation gene expression largely unknown. Here, we performed a hyperinsulinemic-euglycemic clamp on 3-month-old male C57BL/6 mice for 3 h. We show that, comparison with results saline-infused controls, increases within physiological range regulate broad network genes brain. Insulin regulates distinct pathways hypothalamus (HTM), hippocampus, and nucleus accumbens....
Oxidative stress-induced myocardial apoptosis and necrosis are critically involved in ischemic infarction, several sources of extracellular vesicles appear to be enriched therapeutic activities. The central objective was identify validate the differential exosome miRNA repertoire human cardiac progenitor cells (CPC). CPC exosomes were first analyzed by LC-MS/MS compared RNAseq with exomes mesenchymal stromal fibroblasts define their (exo-miRSEL). Proteomics demonstrated a highly significant...
Enhancing thermogenic brown adipose tissue (BAT) function is a promising therapeutic strategy for metabolic disease. However, predominantly thermoneutral modern human living conditions deactivate BAT. We demonstrate that selective adipocyte deficiency of the oxygen-sensor HIF-prolyl hydroxylase (PHD2) gene overcomes BAT dormancy at thermoneutrality. Adipocyte-PHD2-deficient mice maintain higher energy expenditure having greater capacity. In and murine adipocytes, PHD inhibitor increases Ucp1...