A5028742544- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- DNA Repair Mechanisms
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Pluripotent Stem Cells Research
- Congenital heart defects research
- RNA Interference and Gene Delivery
- Cardiac Fibrosis and Remodeling
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Bacterial Genetics and Biotechnology
- Electrospun Nanofibers in Biomedical Applications
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- Viral Infectious Diseases and Gene Expression in Insects
- Hematopoietic Stem Cell Transplantation
- Cancer Cells and Metastasis
- Plant Virus Research Studies
- T-cell and B-cell Immunology
- Telomeres, Telomerase, and Senescence
- Cancer Research and Treatments
Universidad Autónoma de Madrid
2008-2024
Centro Nacional de Biotecnología
2015-2024
National Center for Biotechnology
2021
Spanish National Centre for Cardiovascular Research
2010-2019
Centro de Investigación Biomédica en Red
2017
Centro de Investigación en Red en Enfermedades Cardiovasculares
2017
Hospital General Universitario Gregorio Marañón
2017
Complejo Hospitalario de Navarra
2017
KU Leuven
2017
Consejo Superior de Investigaciones Científicas
2001-2016
The immune synapse is an exquisitely evolved means of communication between T cells and antigen-presenting (APCs) during antigen recognition. Recent evidence points to the transfer RNA via exosomes as a novel mode intercellular communication. Here we show that T, B dendritic contain microRNA (miRNA) repertoires differ from those their parent cells. We investigate whether miRNAs are exchanged cognate interactions, demonstrate existence antigen-driven unidirectional cell APC, mediated by...
Human adult stem cells are being evaluated widely for various therapeutic approaches. Several recent clinical trials have reported their safety, showing them to be highly resistant transformation. The clear similarities between cell and cancer genetic programs nonetheless the basis of a proposal that some could derive from human cells. Here we show although they can managed safely during standard ex vivo expansion period (6-8 weeks), mesenchymal undergo spontaneous transformation following...
The results presented in this paper indicate that the phi 29 DNA polymerase is only enzyme required for efficient synthesis of full length with terminal protein, initiation primer, as additional protein requirement. Analysis activity various vitro replication systems indicates two main reasons are responsible efficiency minimal system: 1) highly processive absence any accessory protein; 2) itself able to produce strand displacement coupled polymerization process. Using primed M13 template,...
Redistribution of specialized molecules in migrating cells develops asymmetry between two opposite cell poles, the leading edge and uropod. We show that acquisition a motile phenotype T lymphocytes results asymmetric redistribution ganglioside GM3- GM1-enriched raft domains to uropod, respectively. This segregation each pole parallels specific membrane proteins associated subfraction. Our data suggest partitioning is major determinant for protein polarized cells, as ectopic expression...
In most clinical trials, human mesenchymal stem cells (hMSCs) are expanded in vitro before implantation. The genetic stability of is critical for their use. However, the relationship between stem-cell expansion and poorly understood. Here, we demonstrate that within normal period, hMSC cultures show a high percentage aneuploid progressively increases until senescence. Despite this accumulation, heterogeneous culture senescence-prone subpopulation has lower proliferation potential higher...
Mesenchymal stem cells (MSCs) possess unique paracrine and immunosuppressive properties, which make them useful candidates for cellular therapy. Here, we address how senescence influences the therapeutic potential of human MSCs (hMSCs). Senescence was induced in bone marrow-derived hMSC cultures with gamma irradiation. Control senescent were tested their immunoregulatory activity vitro vivo, an extensive molecular characterization phenotypic changes by performed. We also compared gene...
Background We previously reported the in vitro spontaneous transformation of human mesenchymal stem cells (MSC) generating a population with tumorigenic potential, that we termed transformed (TMC). Methodology/Principal Findings Here have characterized molecular changes associated TMC generation. Using microarrays techniques identified set altered pathways and greater number downregulated than upregulated genes during MSC transformation, part due to expression many untranslated RNAs MSC....
miR-133a and miR-1 are known as muscle-specific microRNAs that involved in cardiac development pathophysiology. We have shown both early progressively upregulated during vitro differentiation of adult progenitor cells (CPCs), but only expression was enhanced under oxidative stress. demonstrated to favor CPCs, whereas overexpression protected CPCs against cell death, targeting, among others, the proapoptotic genes Bim Bmf. miR-133a-CPCs clearly improved function a rat myocardial infarction...
Abstract Gold glyconanoparticles (GNPs) have been prepared as new multivalent tools that mimic glycosphingolipids on the cell surface. GNPs are highly soluble under physiological conditions, stable against enzymatic degradation and nontoxic. Thereby open up a novel promising platform for biological applications. It has recently demonstrated specific tumor‐associated carbohydrate antigens (glycosphingolipids glycoproteins) involved in initial step of tumor spreading. A mouse melanoma model...
The bacteriophage phi 29 DNA polymerase, involved both in the protein-primed initiation and elongation steps of viral replication, displays a very processive 3',5'-exonuclease activity acting preferentially on single-stranded DNA. This exonucleolytic showed marked preference for excision mismatched versus correctly paired 3' terminus. These characteristics enable polymerase to act as proofreading enzyme. A comparative analysis wild-type mutant lacking indicated that productive coupling...
Abstract MicroRNAs, small noncoding RNAs, regulate gene expression primarily at the posttranscriptional level. We previously found that miR-335 is critically involved in regulation and differentiation capacity of human mesenchymal stem cells (hMSCs) vitro. In this study, we investigated significance for therapeutic potential hMSCs. Analysis hMSCs ex vivo culture demonstrated a significant progressive increase prevented by telomerase. Expression levels were also positively correlated with...