A5052729433- Bacteriophages and microbial interactions
- DNA Repair Mechanisms
- Bacterial Genetics and Biotechnology
- Advanced biosensing and bioanalysis techniques
- Erythrocyte Function and Pathophysiology
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- Neonatal Health and Biochemistry
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Polyomavirus and related diseases
Universidad Autónoma de Madrid
1988-1991
Centro de Biología Molecular Severo Ochoa
1988-1991
Consejo Superior de Investigaciones Científicas
1989
University of Bristol
1989
The results presented in this paper indicate that the phi 29 DNA polymerase is only enzyme required for efficient synthesis of full length with terminal protein, initiation primer, as additional protein requirement. Analysis activity various vitro replication systems indicates two main reasons are responsible efficiency minimal system: 1) highly processive absence any accessory protein; 2) itself able to produce strand displacement coupled polymerization process. Using primed M13 template,...
(1) We have prepared murine monoclonal antibodies to the membrane domain of human erythrocyte anion transport protein (band 3). (2) All these react with regions located at cytoplasmic surface red cell. (3) One reacts an epitope present on a loop between C-terminus and point 168 amino acids from C-terminus. The other recognize different epitopes C-terminal tail sequences likely be involved in are defined. (4) Our results show that red-cell is side membrane. (5) None inhibited sulphate...
Protein p5 is a Bacillus subtilis phage phi 29-encoded protein required for 29 DNA replication in vivo. has single-stranded binding (SSB) capacity and stimulates vitro severalfold when polymerase used to replicate either the natural template or primed M13 (ssDNA). Furthermore, other SSB proteins, including Escherichia coli SSB, T4 gp32, adenovirus DNA-binding protein, human factor A, can functionally substitute p5. The stimulatory effect of not due an increase rate. When both competitor...