A5007657618- Pancreatic function and diabetes
- Diabetes and associated disorders
- Telomeres, Telomerase, and Senescence
- Diabetes Management and Research
- Metabolism, Diabetes, and Cancer
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Adipose Tissue and Metabolism
- Calcium signaling and nucleotide metabolism
- Dietary Effects on Health
- Protein Degradation and Inhibitors
- Birth, Development, and Health
- Molecular Biology Techniques and Applications
- Diet and metabolism studies
- Growth Hormone and Insulin-like Growth Factors
- Biomedical Ethics and Regulation
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Fibroblast Growth Factor Research
- Organ Donation and Transplantation
- Diet, Metabolism, and Disease
- Innovation and Socioeconomic Development
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced Glycation End Products research
Joslin Diabetes Center
2015-2024
Harvard University
2015-2024
Kitano Hospital
2023
Universidad Cristiana Autónoma de Nicaragua
2023
The University of Texas Health Science Center at San Antonio
2023
Jackson Laboratory
2023
Harvard Stem Cell Institute
2017
Universidad Nacional Autónoma de México
2006
We report on a hitherto poorly characterized class of genes that are expressed in all tissues, except one. Often, these have been classified as housekeeping genes, based their nearly ubiquitous expression. However, the specific repression one tissue defines special “disallowed genes.” In this paper, we used intersection-union test to screen for such multi-tissue panel genome-wide mRNA expression data. propose disallowed need be repressed target ensure correct function. provide mechanistic...
Neonatal β cells do not secrete glucose-responsive insulin and are considered immature. We previously showed the transcription factor MAFA is key for functional maturation of cells, but physiological regulators this process unknown. Here we show that postnatal rat express thyroid hormone (TH) receptor isoforms deiodinases in an age-dependent pattern as glucose responsiveness develops. In vivo neonatal triiodothyronine supplementation TH inhibition, respectively, accelerated delayed metabolic...
Chronological age (CA) is determined by time of birth, whereas biological (BA) based on changes a cellular level and strongly correlates with morbidity, mortality, longevity. Type 2 diabetes (T2D) associates increased morbidity mortality; thus, we hypothesized that BA would be calculated it from biomarkers collected at routine clinical visits. Deidentified data was obtained three cohorts patients (20-80 years old)-T2D, type 1 (T1D), prediabetes-and compared to gender- age-matched...
Background The type 2 iodothyronine deiodinase (D2) converts the pro-hormone thyroxine into T3 within target tissues. D2 is essential for a full thermogenic response of brown adipose tissue (BAT), and mice with disrupted Dio2 gene (D2KO) have an impaired to cold. BAT also activated by overfeeding. Methodology/Principal Findings After 6-weeks HFD feeding D2KO gained 5.6% more body weight had 28% tissue. Oxygen consumption (V02) was not different between genotypes, but increased respiratory...
The aging of pancreatic β-cells may undermine their ability to compensate for insulin resistance, leading the development type 2 diabetes (T2D). Aging acquire markers cellular senescence and develop a senescence-associated secretory phenotype (SASP) that can lead dysfunction neighboring cells through paracrine actions, contributing β-cell failure. In this study, we defined SASP signature based on unbiased proteomic analysis conditioned media obtained from mouse human senescent chemically...
Senescence in pancreatic beta cells plays a major role cell dysfunction, which leads to impaired glucose homeostasis and diabetes. Therefore, prevention of senescence could reduce the risk Treatment nonobese diabetic (NOD) mice, model type 1 autoimmune diabetes (T1D), with palmitic acid hydroxy stearic acids (PAHSAs), novel class endogenous lipids antidiabetic antiinflammatory effects, delays onset reduces incidence T1D from 82% vehicle treatment 35% PAHSAs. Here, we show that mechanism by...
Function and structure of adult pancreatic islets are determined by early postnatal development, which in rats corresponds to the first month life. We analyzed changes blood glucose hormones during this stage their association with morphological functional alpha beta cell populations period. At day 20 (d20), insulin plasma levels were two- six-fold higher, respectively, as compared d6. Interestingly, period is characterized physiological hyperglycemia hyperinsulinemia, where peripheral...
Abstract There is growing information about the heterogeneity of pancreatic β-cells and how it relates to insulin secretion. This study used approach flow cytometry sort analyze from transgenic mice expressing green fluorescent protein (GFP) under control mouse I gene promoter. Three populations with differing GFP brightness could be identified, which were classified as GFP-low, GFP-medium, GFP-bright. The GFP-medium population comprised 70% total. GFP-low had less secretion determined by...
Context: Human embryonic stem cells (hESCs) differentiated toward β-cells and fetal human pancreatic islet resemble each other transcriptionally are characterized by immaturity with a lack of glucose responsiveness, low levels insulin content, impaired proinsulin-to-insulin processing. However, their response to stimuli that promote functionality have not been compared. Objective: The objective the study was evaluate effects our previous strategies for functional maturation developed in...
Oxidative stress has been associated with insulin resistance and type 2 diabetes. However, it is not clear whether oxidative damage a cause or consequence of the metabolic abnormalities present in diabetic subjects. The goal this study was to determine inducing through genetic ablation superoxide dismutase 1 (SOD1) leads glucose homeostasis. We studied SOD1-null mice wild-type (WT) littermates. Glucose tolerance evaluated intraperitoneal tests. Peripheral hepatic sensitivity quantitated...