A5005701700- Effects of Radiation Exposure
- Inflammatory Bowel Disease
- Radiation Therapy and Dosimetry
- Pancreatic function and diabetes
- Helicobacter pylori-related gastroenterology studies
- Radiation Dose and Imaging
- Spaceflight effects on biology
- High Altitude and Hypoxia
- Microscopic Colitis
- Molecular Biology Techniques and Applications
- Anesthesia and Neurotoxicity Research
- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Radiation Effects and Dosimetry
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Birth, Development, and Health
- Cancer-related Molecular Pathways
- Health, Environment, Cognitive Aging
- Radioactivity and Radon Measurements
- Plant Genetic and Mutation Studies
Belgian Nuclear Research Centre
2016-2025
KU Leuven
2005-2013
National Institute of Environmental Health Sciences
2010
Triangle
2010
<h3>Background and aims:</h3> Infliximab is an effective treatment for ulcerative colitis with over 60% of patients responding to up 30% reaching remission. The mechanism resistance anti-tumour necrosis factor α (anti-TNFα) unknown. This study used colonic mucosal gene expression provide a predictive response signature infliximab in colitis. <h3>Methods:</h3> Two cohorts who received their first refractory were studied. Response was defined as endoscopic histological healing. Total RNA from...
Antimicrobial peptides (AMPs) protect the host intestinal mucosa against microorganisms. Abnormal expression of defensins was shown in inflammatory bowel disease (IBD), but it is not clear whether this a primary defect. We investigated impact anti-inflammatory therapy with infliximab on mucosal gene AMPs IBD.Mucosal 81 assessed 61 IBD patients before and 4-6 weeks after their first infusion 12 control patients, using Affymetrix arrays. Quantitative real-time reverse-transcription PCR...
Interleukin-6 (IL-6) is systemically elevated in obesity and a predictive factor to develop type 2 diabetes. Pancreatic islet pathology diabetes characterized by reduced β-cell function mass, an increased proportion of α-cells relative β-cells, α-cell dysfunction. Here we show that the α cell primary target IL-6 actions. Beginning with investigating tissue-specific expression pattern receptor (IL-6R) both mice rats, find highest IL-6R endocrine pancreas, on α-cell. The functional, acutely...
Cellular redox state is an important metabolic variable, influencing many aspects of cell function like growth, apoptosis, and reductive biosynthesis. In this report, we identify NADPH as a candidate signaling molecule for exocytosis in neuroendocrine cells. pancreatic β-cells, glucose acutely raised the NADPH-to-NADP+ ratio stimulated insulin release parallel. Furthermore, intracellular addition directly granules. Effects on are proposed to be mediated by proteins glutaredoxin (GRX)...
Glucose homeostasis is critically dependent on insulin release from pancreatic β-cells, which strictly regulated by glucose-induced oscillations in membrane potential (V m ) and the cytosolic calcium level ([Ca 2+ ] cyt ). We propose that TRPM5, a Ca -activated monovalent cation channel, positive regulator of release. Immunofluorescence revealed expression TRPM5 islets. A nonselective current with TRPM5-like properties significantly reduced Trpm 5 −/− cells. -imaging electrophysiological...
We report on a hitherto poorly characterized class of genes that are expressed in all tissues, except one. Often, these have been classified as housekeeping genes, based their nearly ubiquitous expression. However, the specific repression one tissue defines special “disallowed genes.” In this paper, we used intersection-union test to screen for such multi-tissue panel genome-wide mRNA expression data. propose disallowed need be repressed target ensure correct function. provide mechanistic...
Infliximab (IFX) has become the mainstay of therapy refractory Crohn's disease (CD). However, a subset patients shows incomplete or no response to this agent. In study we investigated whether could identify mucosal gene panel predict (non)response IFX in CD.Mucosal biopsies were obtained during endoscopy from 37 with active CD (19 colitis [CDc] and 18 ileitis [CDi]) before after first treatment. Response was defined based on endoscopic histologic findings. Total RNA analyzed Affymetrix Human...
OBJECTIVES: Inflammatory bowel disease (IBD) is characterized by a continuous influx of leukocytes into the gut wall. This migration regulated cell adhesion molecules (CAMs), and selective antimigration therapies have been developed. study investigated effect infliximab therapy on mucosal gene expression CAMs in IBD. METHODS: Mucosal 69 leukocyte/endothelial E-cadherin was 61 IBD patients before after first infusion 12 normal controls, using Affymetrix microarrays. Quantitative reverse...
The risk of a large-scale event leading to acute radiation exposure necessitates the development high-throughput methods for providing rapid individual dose estimates. Our work addresses three goals, which align with directive European Union's Realizing Network Biodosimetry project (EU-RENB): 1. To examine suitability different gene expression platforms biodosimetry purposes; 2. perform this examination using blood samples collected from prostate cancer patients (in vivo) and healthy donors...
Furin is a proprotein convertase which activates variety of regulatory proteins in the constitutive exocytic and endocytic pathway. The effect genetic ablation fur was studied endocrine pancreas to define its physiological function regulated secretory Pdx1-Cre/loxP furin KO mice show decreased secretion insulin impaired processing known PC2 substrates like proPC2 proinsulin II. Both activity depend on granule acidification, demonstrated be significantly furin-deficient beta cells by using...
A differentiated β-cell results not only from cell-specific gene expression, but also cell-selective repression of certain housekeeping genes. Indeed, to prevent insulin toxicity, β-cells should handle stores carefully, preventing exocytosis under conditions when circulating is unwanted. Some ubiquitously expressed proteins would significantly jeopardize this safeguard, allowed function in β-cells. This illustrated by two studied examples. First, low-Km hexokinases are disallowed as their...
Oxidative phosphorylation in mitochondria is responsible for 90% of ATP synthesis most cells. This essential housekeeping function mediated by nuclear and mitochondrial genes encoding subunits complex I to V the respiratory chain. Although IV best studied these complexes, exact striated muscle-specific subunit COX6A2 still poorly understood. In this study, we show that Cox6a2-deficient mice are protected against high-fat diet-induced obesity, insulin resistance glucose intolerance. phenotype...
Purpose: A European network was initiated in 2012 by 23 partners from 16 countries with the aim to significantly increase individualized dose reconstruction case of large-scale radiological emergency scenarios.Results: The built on three complementary pillars: (1) an operational basis seven biological and physical dosimetric assays ready-to-use mode, (2) a for education, training quality assurance, (3) further development regarding new techniques members. Techniques individual estimation...
Purpose: RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research emergency response mutual assistance in biodosimetry within EU. Within this extremely active network, number new dosimetry methods have recently been proposed or developed. There requirement to test and/or validate these candidate techniques inter-comparison exercises are well-established method such validation.Materials methods: The authors present details...
Abstract Accurate assessment of the individual exposure dose based on easily accessible samples (e.g. blood) immediately following a radiological accident is crucial. We aimed at developing robust transcription-based signature for biodosimetry from human peripheral blood mononuclear cells irradiated with different doses X-rays (0.1 and 1.0 Gy) rate 0.26 Gy/min. Genome-wide radiation-induced changes in mRNA expression were evaluated both gene exon level. Using exon-specific qRT-PCR, we...
This collaboration of five established European gene expression labs investigated the potential impact culture conditions on transcriptional response peripheral blood to radiation exposure.Blood from one healthy donor was exposed ex vivo a Cobalt 60 source produce calibration curve in addition four unknown doses. After exposure, samples were either diluted with RPMI medium or left untouched. 24-h incubation at 37 °C lysed, while undiluted mixed preservative RNALater and all shipped frozen...
Abstract Embryonic DNA damage resulting from repair deficiencies or exposure to ionizing radiation during early neurogenesis can lead neurodevelopmental disorders, including microcephaly. This has been linked an excessive response in dorsal neural progenitor cells (NPCs), p53-dependent apoptosis and premature neuronal differentiation which culminates depletion of the NPC pool. However, effect on ventral forebrain NPCs, origin interneurons, remains unclear. In this study, we investigated...