A5090159945- Neuroscience and Neuropharmacology Research
- Epilepsy research and treatment
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Regulation of Appetite and Obesity
- Pharmacological Effects and Toxicity Studies
- Renin-Angiotensin System Studies
- Mass Spectrometry Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Ion channel regulation and function
- Stress Responses and Cortisol
- Pharmacological Effects and Assays
- Biochemical Analysis and Sensing Techniques
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Neurotransmitter Receptor Influence on Behavior
- Neurological disorders and treatments
- Neuroendocrine regulation and behavior
- Tryptophan and brain disorders
- Hormonal Regulation and Hypertension
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Neuroscience of respiration and sleep
- Anesthesia and Pain Management
Vrije Universiteit Brussel
2016-2025
Modulation Therapeutics (United States)
2008-2020
Center for Neurosciences
2020
Digital Research Alliance of Canada
2019
Metropolitan University
2018
Pharmac
2012-2018
Leiden University Medical Center
2017
Center for Beta Cell Therapy in Diabetes
2016
Universitair Ziekenhuis Brussel
2008-2015
Geneeskundige en Gezondheidsdienst
2006-2015
Zebrafish have recently emerged as an attractive in vivo model for epilepsy. Seven-day-old zebrafish larvae exposed to the GABA(A) antagonist pentylenetetrazol (PTZ) exhibit increased locomotor activity, seizure-like behavior, and epileptiform electrographic activity. A previous study showed that 12 out of 13 antiepileptic drugs (AEDs) suppressed PTZ-mediated increases larval movement, indicating potential utility a high-throughput AED discovery. However, question remained whether...
Vagus nerve stimulation (VNS) is an effective adjunctive treatment for medically refractory epilepsy. In this study, we measured VNS-induced changes in hippocampal neurotransmitter levels and determined their potential involvement the anticonvulsive action of VNS, to elucidate mechanism responsible seizure suppressing effect VNS animal model limbic seizures. We used vivo intracerebral microdialysis measure extracellular concentrations noradrenaline, dopamine, serotonin GABA freely moving,...
Endothelial cells (ECs) are highly glycolytic and generate the majority of their energy via breakdown glucose to lactate. At same time, a main role ECs is allow transport surrounding tissues. GLUT1 (glucose transporter isoform 1/Slc2a1) expressed in central nervous system (CNS) often implicated blood-brain barrier (BBB) dysfunction, but whether how controls EC metabolism function poorly understood.We evaluated endothelial during postnatal CNS development as well at adult BBB.Inhibition...
MEEUSEN, R., SMOLDERS, I., SARRE, S., DE MEIRLEIR, K., KEIZER, H., SERNEELS, M., EBINGER, G. & MICHOTTE, Y. 1997. Endurance training effects on neurotransmitter release in rat stratium – an vivo microdialysis study. Acta Physiol Scand 159 , 335–341. Received 14 May 1996, accepted 15 November 1996. ISSN 0001–6772. Department of Human Physiology and Sports Medicine, Pharmaceutical Chemistry Drug Analysis, Neurology, University Hospital, Vrije Universiteit Brussel, Brussels, Belgium;...
System x c − exchanges intracellular glutamate for extracellular cystine, giving it a potential role in glutathione synthesis and nonvesicular release. We report that mice lacking the specific xCT subunit of system ( −/− ) do not have lower hippocampal content, increased oxidative stress or brain atrophy, nor exacerbated spatial reference memory deficits with aging. Together these results indicate loss does induce vivo . Young did however display working deficit. Interestingly, we observed...
Dravet syndrome (DS) is a genetic encephalopathy that characterized by severe seizures and prominent co-morbidities (e.g. physical, intellectual disabilities). More than 85% of the DS patients carry an SCN1A mutation (sodium channel, voltage gated, type I alpha subunit). Although numerous anti-epileptic drugs (AEDs) have entered market since 1990, these often fail to adequately control in patients. Nonetheless, current clinical data shows significant seizure reduction treated with...
Dravet syndrome (DS) is a severe epilepsy that starts within the first year of life. In clinical study, add-on treatment with fenfluramine, potent 5-hydroxytryptamine (5-HT) releaser activating multiple 5-HT receptor subtypes, made 70% DS children seizure free. Others and we recently confirmed efficacy fenfluramine as an antiepileptiform compound in zebrafish models DS. By using large set subtype selective agonists, this study examined which subtypes can be targeted to trigger antiseizure...
Abstract Background Epileptic seizures are an established comorbidity of Alzheimer’s disease (AD). Subclinical epileptiform activity (SEA) as detected by 24-h electroencephalography (EEG) or magneto-encephalography (MEG) has been reported in temporal regions clinically diagnosed AD patients. Although epileptic probably arises the mesial lobe, electrical within this region might not propagate to EEG scalp electrodes and could remain undetected standard EEG. However, SEA lead faster cognitive...
Pilocarpine administration has been used as an animal model for temporal lobe epilepsy since it produces several morphological and synaptic features in common with human complex partial seizures. Little is known about changes extracellular neurotransmitter concentrations during the seizures provoked by pilocarpine, a non‐selective muscarinic agonist. Focally evoked pilocarpine‐induced freely moving rats were intrahippocampal pilocarpine (10m m 40min at flow rate of 2μl min −1 ) via...
Abstract The present microdialysis study evaluated the anticonvulsant activity of extracellular hippocampal dopamine (DA) and serotonin (5‐HT) with concomitant assessment possible mutual interactions between these monoamines. effects intrahippocampally applied DA 5‐HT concentrations were against pilocarpine‐induced seizures in conscious rats. or perfusions protected rats from limbic as long ranged, respectively, 70–400% 80–350% increases compared baseline levels. Co‐perfusion selective D 2...
Malfunctioning of system xc–, responsible for exchanging intracellular glutamate extracellular cystine, can cause oxidative stress and excitotoxicity, both important phenomena in the pathogenesis Parkinson's disease (PD). We used mice lacking xCT (xCT_/_ mice), specific subunit xc˜, to investigate involvement this antiporter PD. Although cystine that is imported via xc˜ reduced cysteine, rate-limiting substrate synthesis glutathione, deletion did not result decreased glutathione levels...
Using 8- and 18-month-old AβPP23 mice, we investigated the involvement of high-affinity glutamate transporters (GLAST, GLT-1, EAAC1), vesicular (VGLUT1-3) xCT, specific subunit system xc − , in Alzheimer's disease (A
Human embryonic stem cells (hESC) show great promise for clinical and research applications, but their well-known proneness to genomic instability hampers the development full potential. Here, we demonstrate that medium acidification linked culture density is main cause of DNA damage alterations in hESC grown on feeder layers, this even short time span a single passage. In line with this, increasing frequency refreshments minimizes levels genetic instability. Also, cultured laminin-521 do...