A5065160061- Neuroscience and Neuropharmacology Research
- Pancreatic function and diabetes
- Pancreatitis Pathology and Treatment
- Calcium signaling and nucleotide metabolism
- Cellular transport and secretion
- Amino Acid Enzymes and Metabolism
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Neuropeptides and Animal Physiology
- Ion Channels and Receptors
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Adenosine and Purinergic Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Neural dynamics and brain function
- Supramolecular Self-Assembly in Materials
- Biochemical Analysis and Sensing Techniques
- Nerve injury and regeneration
- Neuroendocrine Tumor Research Advances
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Tryptophan and brain disorders
- Diabetes Treatment and Management
- bioluminescence and chemiluminescence research
- Alzheimer's disease research and treatments
Kyoto Prefectural University of Medicine
2025
University of Catania
2023
Université de Bordeaux
2017-2023
Centre de Recherche Inria Bordeaux - Sud-Ouest
2023
Institut national de recherche en informatique et en automatique
2023
Inserm
2017-2023
Neurocentre Magendie
2017-2023
Institut de Mathématiques de Bordeaux
2023
University of Liverpool
2006-2018
NIHR Liverpool Pancreatic Biomedical Research Unit
2018
The intracellular activation of trypsinogen, which is both pH- and calcium-dependent, an important early step in the development acute pancreatitis. cellular compartment trypsinogen occurs currently unknown. We therefore investigated site by using established model pancreatitis: supramaximal stimulation pancreatic acinar cells with cholecystokinin. used fluorescent dextrans as fluid phase tracers observed cholecystokinin-elicited formation translocation large endocytic vacuoles. probe...
Astrocytes regulate hippocampal synaptic plasticity by the Ca 2+ dependent release of N ‐methyl d ‐aspartate receptor (NMDAR) co‐agonist ‐serine. Previous evidence indicated that ‐serine would be regulated intracellular channel IP 3 (IP R), however, genetic deletion R2, putative astrocytic R subtype, had no impact on or transmission. Although R2 is widely believed to only functional in astrocytes, three subtypes (1, 2, and 3) have been identified vertebrates. Therefore, better understand...
GABAergic synaptic transmission regulates brain function by establishing the appropriate excitation-inhibition (E/I) balance in neural circuits. The structure and of synapses are sensitive to destabilization impinging neurotransmitters. However, signaling mechanisms that promote restorative homeostatic stabilization remain unknown. Here, quantum dot single-particle tracking, we characterize a pathway promotes stability GABAA receptor (GABAAR) postsynaptic organization. Slow metabotropic...
Inositol trisphosphate and cyclic ADP-ribose release Ca2+ from the endoplasmic reticulum via inositol ryanodine receptors, respectively. By contrast, nicotinic acid adenine dinucleotide phosphate may activate a novel channel in an compartment. We show, two-photon permeabilized pancreatic acinar cells, that three messengers tested could each also store granular region. The action on both types of store, like but unlike trisphosphate, depended operational since it was blocked by or ruthenium...
Toxic alcohol effects on pancreatic acinar cells, causing the often fatal human disease acute pancreatitis, are principally mediated by fatty acid ethyl esters (non-oxidative products of and acids), emptying internal stores Ca(2+). This excessive Ca(2+) liberation induces Ca(2+)-dependent necrosis due to intracellular trypsin activation. Our aim was identify specific source release linked protease In 2-photon permeabilized mouse we monitored changes in concentration thapsigargin-sensitive...
An mGluR5-selective diffusion barrier enriches mGluR5 in astrocytic processes, enabling compartmentalized calcium signaling.
Intracellular Ca(2+) release is mostly mediated by inositol trisphosphate, but intracellular cyclic-ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) are important messengers in many systems. Whereas cADPR generally activates type 2 ryanodine receptors (RyR2s), the NAADP-activated mechanism less clear. Using knockouts antibodies against RyRs Two-Pore Channels (TPCs), we have compared their relative importance for NAADP-induced from two-photon permeabilized...
Neural reflexes to chemicals in the throat protect airway from aspiration and infection. Mechanistic understanding of these remains premature, exemplified by chronic cough-a sensitized cough reflex-being a prevalent unmet clinical need. Here, mice, whole-body search for channel synapses-featuring CALHM1/3 channel-mediated neurotransmitter release-and single-cell transcriptomics uncovered subclasses Pou2f3+ chemosensory cell family communicating with vagal neurons via this synapse. They...
Orai1 proteins have been recently identified as subunits of SOCE (store-operated Ca²⁺ entry) channels. In primary isolated PACs (pancreatic acinar cells), showed remarkable co-localization and co-immunoprecipitation with all three subtypes IP₃Rs (InsP₃ receptors). The between was restricted to the apical part PACs. Neither nor affected by store depletion. Importantly we also characterized in basal lateral membranes shown previously accumulate STIM1 (stromal interaction molecule 1) puncta a...
Alcohol abuse is a major global health problem, but there still much uncertainty about the mechanisms of action. So far, effects ethanol on ion channels in plasma membrane have received most attention. We now investigated actions intracellular calcium pancreatic acinar cells. Our aim was to discover mechanism by which alcohol influences homeostasis and thereby understand how can trigger premature trypsinogen activation, initiating step for alcohol-induced pancreatitis. used intact or...
ADP‐ribosylation factor (ARF) proteins are involved in multiple intracellular vesicular transport pathways. Most studies have focused on the functions of ARF1 or ARF6 and little is known about remaining ARF isoforms. Although mammalian share a high degree sequence identity, recent evidence has indicated that they may control distinct trafficking steps within cells. A unanswered issue specificity family members for different interacting proteins. To investigate potential functional...
Giant trypsin-containing endocytic vacuoles are formed in pancreatic acinar cells stimulated with inducers of acute pancreatitis. F-actin envelops and regulates their properties. Endocytic can rupture release content into the cytosol cells. fuse plasma membrane exocytose content.Intrapancreatic activation trypsinogen is an early event hallmark development vacuoles, which form by disconnection transport large post-exocytic structures, only resolvable sites trypsin activity live In present...
Here we describe a technique that allows us to visualize in real time the formation and dynamics (fusion, changes of shape, translocation) vacuoles living cells. The involves infusion dextran-bound fluorescent probe into cytosol cell via patch pipette, using whole-cell patch-clamp configuration. Experiments were conducted on pancreatic acinar cells stimulated with supramaximal concentrations cholecystokinin (CCK). vacuoles, forming cytoplasm cell, revealed as dark imprints bright...
Background & Aims: How cholecystokinin (CCK) stimulates human pancreatic acinar cells has not been determined, so we sought to discover whether this occurs through cell CCK receptors. Methods: Human were freshly isolated from transection line samples, loaded with Fluo4-AM, and examined for calcium responses or acetylcholine (ACh) confocal microscopy. Stimulus-metabolism coupling was assessed NADH autofluorescence trypsinogen activation tested BZiPAR. Results: (10-20 pM) induced rapid,...
For many years, voltage sensitive dye imaging (VSDI) has enabled the fruitful analysis of neuronal transmission by monitoring spreading signals. Although useful, display diffusion depolarization provides insufficient information in quest for a greater understanding computation brain function. Here, we propose optimal mass transportation theory as model to describe dynamics activity. More precisely, use solution an L 2 -Monge–Kantorovich problem VSDI data, extract velocity and overall...
JapanGeneral IncorporatedAssociation animals isolated from U.K. were destroyed by cold stimuli, 2℃ for 48 hours.By contrast, most of wild-type can survive hours at , afier cultivation 15 degree.Detailed temperature-changes experiments revea]ed that the cold-resistance is established temperature memory adult stage, We measured in various mutant defective sensoryneurons, Abnormal enhancement found TAX-4 cGMP-gated channel, which expressed 10 pairs sensoryneurons including thermo-sensoyneuron...
Background & Aims: We have previously reported Ca2+ responses to cholecystokinin (CCK) in isolated human pancreatic acinar cells, but function has not been proven. sought determine whether cells functioning CCK receptors. Methods: Human were freshly from transection line samples, loaded with Fluo4-AM or quinacrine, and examined for Ca2+, metBDolic secretory CCK-8, CCK-58 ACh confocal microscopy. Results: CCK-8 at physiological concentrations (1-20 pM) elicited rapid, robust, oscillatory...