A5005167203- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Microtubule and mitosis dynamics
- Retinal Development and Disorders
- Erythrocyte Function and Pathophysiology
- Receptor Mechanisms and Signaling
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Neurobiology and Insect Physiology Research
- Calcium signaling and nucleotide metabolism
- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- Signaling Pathways in Disease
- Photoreceptor and optogenetics research
- Ion Channels and Receptors
- S100 Proteins and Annexins
- Adenosine and Purinergic Signaling
- Protein Kinase Regulation and GTPase Signaling
- Ubiquitin and proteasome pathways
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Biochemical Analysis and Sensing Techniques
- Neuropeptides and Animal Physiology
University of Liverpool
2011-2022
KU Leuven
2009
University College London
2004
MRC Laboratory of Molecular Biology
2002
Medical Research Council
1987-2002
University of California, Davis
2001
University of Glasgow
2001
European Molecular Biology Laboratory
2001
Physiological Society
1994-1996
University of Birmingham
1976-1994
Ca(2+) plays a central role in the function of neurons as trigger for neurotransmitter release, and many aspects neuronal activity, from rapid modulation to changes gene expression, are controlled by Ca(2+). These actions must be mediated Ca(2+)-binding proteins, including calmodulin, which is involved regulation, not only neurons, but most other cell types. A large number EF-hand-containing proteins known. One family these, calcium sensor (NCS) has restricted expression retinal...
Lipid rafts are microdomains present within membranes of most cell types. These membrane microdomains, which enriched in cholesterol and glycosphingolipids, have been implicated the regulation certain signal transduction traffic pathways. To investigate possibility that lipid organize exocytotic pathways neuroendocrine cells, we examined association proteins machinery with purified from PC12 cells. The target soluble N- ethylmaleimide-sensitive factor attachment protein receptor (tSNARE)...
The effect of endogenous glutamate on neurite outgrowth from cerebellar granule cells in culture was examined. Neurite inhibited by enzymatic removal the medium. broad‐spectrum receptor antagonist kynurenate also serum‐containing and serum‐free cultures; inhibition reversed exogenous glutamate. to same extent NMDA APV. These results indicate that glutamate, possibly released themselves, stimulated through activation class receptors. Activation receptors developing neurons may be an important...
Intracellular membrane fusion is mediated by the SNARE (soluble N -ethylmaleimide–sensitive factor attachment protein receptor) proteins. All vesicle transport steps also have an essential requirement for a member of Sec1 family, including neuronal Munc18-1 (also known as nSec1) in regulated exocytosis. Here, adrenal chromaffin cells, we expressed Munc18 mutant with reduced affinity syntaxin, which specifically modified kinetics single-granule exocytotic release events, consistent...
We have used stage-specific assays for MgATP-dependent priming and Ca(2+)-activated triggering in the absence of free MgATP to examine effects alpha-SNAP, 14-3-3 proteins calmodulin on regulated exocytosis permeabilized adrenal chromaffin cells. All three lead a Ca(2+)-dependent increase catecholamine secretion. Both alpha-SNAP stimulated but not assay. In contrast, was stimulatory priming. The were likely be due distinct mechanisms action since they differed Ca(2+)-dependency, time course...
Using rhodamine-phalloidin staining it was found that actin filaments are concentrated in the cortex of resting chromaffin cells. Cortical were disassembled 15 s after stimulation by nicotine and had reassembled 30 later. Actin filament disassembly following nicotinic also detected using DNase I inhibition assay. Disassembly independent external calcium, insensitive to trifluoperazine not elicited high K+, muscarinic agonists or phorbol ester. cortical may allow access secretory granules...
The distribution of acetylated alpha-tubulin in rat cerebellum was examined and compared with that total tyrosinated alpha-tubulin. From immunoperoxidase-stained vibratome sections it found alpha-tubulin, detectable monoclonal 6-11B-1, preferentially enriched axons dendrites. Parallel fiber axons, particular, were labeled 6-11B-1 yet unstained by an antibody recognizing indicating parallel fibers contain is detyrosinated. Axonal microtubules are known to be highly stable the other classes...
Ca2+ entry through store-operated channels involves the interaction at ER-PM (endoplasmic reticulum-plasma membrane) junctions of STIM (stromal molecule) and Orai. proteins are sensors luminal ER concentration and, following depletion Ca2+, they oligomerize translocate to where form puncta. Direct binding Orai activates their channel function. It has been suggested that an additional C-terminal polybasic domain STIM1 with PM phosphoinositides could contribute puncta formation prior In...