A5045834284- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Adenosine and Purinergic Signaling
- Receptor Mechanisms and Signaling
- Lipid Membrane Structure and Behavior
- Ion Transport and Channel Regulation
- Prenatal Screening and Diagnostics
- Congenital Anomalies and Fetal Surgery
- Fetal and Pediatric Neurological Disorders
- Diet and metabolism studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Pregnancy and preeclampsia studies
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Mitochondrial Function and Pathology
- Supramolecular Self-Assembly in Materials
- Quantum Dots Synthesis And Properties
- Genetics and Neurodevelopmental Disorders
- Cellular transport and secretion
- Congenital Heart Disease Studies
- Birth, Development, and Health
- Cleft Lip and Palate Research
- Advanced Fluorescence Microscopy Techniques
- Retinal Development and Disorders
- Alzheimer's disease research and treatments
Institut du Fer à Moulin
2014-2024
Inserm
2014-2024
Sorbonne Université
2014-2024
Centre National de la Recherche Scientifique
2003-2024
ESPCI Paris
2024
Université Paris Sciences et Lettres
2015-2024
Laboratoire Plasticité du Cerveau
2024
Université Sorbonne Nouvelle
2019
Institut de Biologie de l'École Normale Supérieure
2012-2015
École Normale Supérieure - PSL
1996-2015
Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells periods ranging from milliseconds minutes. characterized multiple diffusion domains relation synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry GlyRs into synapse by was observed further confirmed electron microscopy imaging...
The characterization of the Caenorhabditis elegans unc-47 gene recently allowed identification a mammalian (gamma)-amino butyric acid (GABA) transporter, presumed to be located in synaptic vesicle membrane. In situ hybridization data rat brain suggested that it might also take up glycine and thus represent general Vesicular Inhibitory Amino Acid Transporter (VIAAT). present study, we have investigated localization VIAAT neurons by using polyclonal antibody raised against hydrophilic...
The role of the scaffolding protein gephyrin at hippocampal inhibitory synapses is not well understood. A previous study (Kneussel et al., 1999) reported a complete loss synaptic clusters major GABA R subunits α2 and γ2 in neurons lacking gephyrin. In contrast, we show here that do cluster pyramidal cultures from gephyrin-/- mice, albeit reduced levels compared with control neurons. Synaptic aggregation α1 on interneurons was identical between culture types. Furthermore, recorded miniature...
The K-Cl co-transporter KCC2 plays multiple roles in the physiology of central neurons and alterations its function and/or expression are associated with several neurological conditions. By regulating intraneuronal chloride homeostasis, strongly influences efficacy polarity chloride-permeable γ-aminobutyric acid (GABA) type A glycine receptor (GlyR) mediated synaptic transmission. This appears particularly critical for development neuronal circuits as well dynamic control GABA signaling...
The dystrophin glycoprotein complex (DGC) is a multimolecular that links the extracellular matrix to cytoskeleton. DGC present at skeletal neuromuscular junction and required for its maturation maintenance. Members of are also expressed in brain. We used cultured hippocampal neurons analyze distribution, regulation, role synaptogenesis major transmembrane component DGC, dystroglycan; one ligands, agrin; cytoskeletal binding partners, dystrophin. α-Dystroglycan, β-dystroglycan, clustered...
The K-Cl cotransporter KCC2 plays an essential role in neuronal chloride homeostasis, and thereby influences the efficacy polarity of GABA signaling. Although is expressed throughout somatodendritic membrane, it remarkably enriched dendritic spines, which host most glutamatergic synapses cortical neurons. has been shown to influence spine morphogenesis functional maturation developing neurons, but its function mature spines remains unknown. Here, we report that suppressing expression...
The neuronal K/Cl transporter KCC2 exports chloride ions and thereby influences the efficacy polarity of GABA signaling in brain. is also critical for dendritic spine morphogenesis maintenance glutamatergic transmission cortical neurons. Because plays a pivotal role function central synapses, it particular importance to understand cellular molecular mechanisms underlying its regulation. Here, we studied impact membrane diffusion clustering on function. forms clusters vicinity both excitatory...
The K+-Cl- co-transporter KCC2 (SLC12A5) tunes the efficacy of GABAA receptor-mediated transmission by regulating intraneuronal chloride concentration [Cl-]i. undergoes activity-dependent regulation in both physiological and pathological conditions. synaptic excitation is well documented; however, whether transporter regulated inhibition unknown. Here we report a mechanism receptor (GABAAR)-mediated mature hippocampal neurons. Enhancing GABAAR-mediated confines to plasma membrane, while...
GABAergic synaptic transmission regulates brain function by establishing the appropriate excitation-inhibition (E/I) balance in neural circuits. The structure and of synapses are sensitive to destabilization impinging neurotransmitters. However, signaling mechanisms that promote restorative homeostatic stabilization remain unknown. Here, quantum dot single-particle tracking, we characterize a pathway promotes stability GABAA receptor (GABAAR) postsynaptic organization. Slow metabotropic...
See Cunha (doi:10.1093/brain/awz335) for a scientific commentary on this article. Carvalho et al. provide clues to the onset of immune dysregulation underlying early synaptic loss in Alzheimer’s disease and tauopathies, by linking overactivation adenosine A2A receptors tau pathology particular microglial signature (upregulation C1q TREM2) allied glutamatergic synapses cognitive deficits.
During development, neural circuit formation requires the stabilization of active γ-aminobutyric acid–mediated (GABAergic) synapses and elimination inactive ones. Here, we demonstrate that, although activation postsynaptic GABA type A receptors (GABAARs) adenosine A2A (A2ARs) stabilizes GABAergic synapses, only A2AR is sufficient. Both GABAAR- A2AR-dependent signaling pathways act synergistically to produce 3′,5′-monophosphate through recruitment calcium–calmodulin–adenylyl cyclase pathway....
United States and European consensus views differ on the place of routine ultrasound scans during pregnancy validity such as screening tests for fetal malformations in general population is still under debate. Four laboratories from Obstetric Gynecology departments Belgian University hospitals affiliated have conducted a prospective study 1984 to 1989 compare anomalies discovered ultrasonic fetus with neonates. Of 16,370 pregnant women at normal risk congenital attending antenatal clinics...
Lateral diffusion of neurotransmitter receptors in and out synapses has been postulated as a core mechanism for rapid changes receptor number at during plastic processes. In this study, we have used single particle tracking to investigate how glycine (GlyR) lateral properties might account after disruption the cytoskeleton dissociated spinal cord neurons. We found that pharmacological F-actin microtubules decreased amount GlyR gephyrin, backbone inhibitory postsynaptic scaffold, synapses....
The ultrasonic examination of 6,690 pregnant patients showed a greater number multiple pregnancies than usually observed at delivery: 71 per cent twin gestations diagnosed before 10 weeks were singletons when delivered.
Expression of the neuronal K/Cl transporter KCC2 is tightly regulated throughout development and by both normal pathological activity. Changes in expression have often been associated with altered chloride homeostasis GABA signaling. However, recent evidence supports a role function glutamatergic synapses through mechanisms that remain poorly understood. Here we show suppressing rat hippocampal neurons precludes long-term potentiation specifically preventing activity-driven membrane delivery...
Abstract Five ultrasound laboratories from Obstetrics and Gynecology departments of Belgian university hospitals or affiliated institutions conducted a prospective study 1984 to 1992 in which the results prenatal examinations were compared examination reports neonates. The period 1984–89 (PI) have been published previously, those 1990–92 (PII) are presented here. Some very minor congenital anomalies, as listed. defined EUROCAT Register, excluded. Of 26 147 pregnant women at normal risk for...
ABSTRACT Listeria monocytogenes is an intracellular pathogen that causes severe central nervous system infection in humans and animals. The ability of this bacterium to penetrate nerve cells was investigated by using rat spinal cell cultures. Entry into distinct types, i.e., glial neurons, monitored a differential immunofluorescence technique with antibodies against type-specific markers the bacterial pathogen. L. detected predominantly within macrophages constituting microglia. Astrocytes...
Abstract Synaptic plasticity relies on the rapid changes in neurotransmitter receptor number at postsynaptic sites. Using superresolution photoactivatable localization microscopy imaging and quantum dot–based single-particle tracking rat hippocampal cultured neurons, we investigated whether phosphorylation status of main scaffolding protein gephyrin influenced organization scaffold GABA A (GABA R) membrane dynamics. We found that regulates microdomain compaction. Extracellular...
ABSTRACT The cellular and molecular mechanisms underlying the postsynaptic aggregation of ionotropic receptors in central nervous system are not understood. glycine receptor (GlyR) its cytoplasmic domain-associated protein, gephyrin, clustered at membrane constitute a good model for addressing these questions. is inhibited by strychnine. effects chronic strychnine treatment on expression distribution gephyrin were therefore tested using primary cultures spinal cord neurons. Reverse...
We have evaluated the influence of secretory phenotype presynaptic boutons on accumulation postsynaptic glycine receptors (GlyRs), type A GABA (GABA<sub>A</sub>Rs), and gephyrin clusters. The cellular distribution these components was analyzed motoneurons cultured either alone or with glycinergic and/or GABAergic neurons. In alone, we observed clusters at nonsynaptic sites in front cholinergic boutons, whereas GABA<sub>A</sub> formed These are functionally pharmacologically similar to those...