In BriefO'Leary et al. find a long non-coding RNA called PARTICLE that is overexpressed following irradiation.PARTICLE represses tumor suppressor MAT2A via triplex formation and interaction with the polycomb repressor complex.PARTICLE also acts as cytosolic scaffold for in preparation exosomal transport from cell.

Exosomes are nanometer-sized extracellular vesicles that believed to function as intercellular communicators. Here, we report exosomes able modify the radiation response of head and neck cancer cell lines BHY FaDu. were isolated from conditioned medium irradiated well non-irradiated cells by serial centrifugation. Quantification using NanoSight technology indicated an increased exosome release compared 24 hours after treatment. To test whether released influence other transferred recipient...

The prediction of therapy response in head and neck squamous cell cancer (HNSCC) requires biomarkers, which are also a prerequisite for personalised concepts. current study aimed to identify therapy-responsive microRNAs (miRNAs) the circulation that can serve as minimally invasive prognostic markers HNSCC patients undergoing radiotherapy.We screened plasma miRNAs discovery cohort before after treatment. We further compared age- sex-matched healthy controls. All identified biomarker...

Radiation is a highly efficient therapy in squamous head and neck carcinoma (HNSCC) treatment. However, local recurrence metastasis are common complications. Recent evidence shows that cancer-cell-derived exosomes modify tumour cell movement metastasis. In this study, we link radiation-induced changes of to their ability promote migration recipient HNSCC cells. We demonstrate isolated from irradiated donor cells boost the motility BHY FaDu. Molecular data identified enhanced AKT-signalling,...

The risk of a large-scale event leading to acute radiation exposure necessitates the development high-throughput methods for providing rapid individual dose estimates. Our work addresses three goals, which align with directive European Union's Realizing Network Biodosimetry project (EU-RENB): 1. To examine suitability different gene expression platforms biodosimetry purposes; 2. perform this examination using blood samples collected from prostate cancer patients (in vivo) and healthy donors...

Circulating microRNAs (miRNAs) are easily accessible and have already proven to be useful as prognostic markers in cancer patients. However, their origin function the circulation is still under discussion. In present study we analyzed changes miRNAs blood plasma of head neck squamous cell carcinoma (HNSCC) patients response radiochemotherapy compared them a culture model primary HNSCC cells undergoing simulated anti-cancer therapy. MiRNA-profiles were by qRT-PCR arrays paired samples before...

Epidemiological evidence suggests that low doses of ionising radiation (≤1.0 Gy) produce persistent alterations in cognition if the exposure occurs at a young age. The mechanisms underlying such are unknown. We investigated long-term effects total body gamma on neonatally exposed NMRI mice molecular and cellular level to elucidate neurodegeneration. Significant spontaneous behaviour were observed 2 4 months following single 0.5 or 1.0 Gy exposure. Alterations brain proteome, transcriptome,...

Multiple cell types secrete exosome-like extracellular vesicles (ELVs) to the environment. Pathological conditions can produce characteristic changes vesicle cargo. We investigated if ionizing radiation is capable of inducing in protein and microRNA (miRNA) cargo ELVs.Whole blood samples from healthy donors were irradiated with 2 Gy gamma rays then peripheral mononuclear cells plasma separated residual co-cultivated for 24 h. The released ELVs collected by differential ultracentrifugation...

Extracellular vesicles (EVs) are cell-derived membrane-bound organelles that have generated interest as they reflect the physiological condition of their source. Mass spectrometric (MS) analyses protein cargo EVs may lead to discovery biomarkers for diseases. However, a comprehensive MS-based proteomics analysis, an optimal lysis is required. Six methods extraction from secreted by head and neck cell line BHY were compared. Commercial radioimmunoprecipitation assay (RIPA) buffer outperformed...

A detailed understanding of the mechanisms that determine variable cellular sensitivity to radiation is needed for improved therapy as well identification individuals with innate hypersensitivity. MicroRNAs (miRNAs) are a class small non-coding RNAs post-transcriptionally regulate protein expression. Alterations in miRNA expression patterns response ionizing have been shown, but there almost no data describing functional impact these changes. We report here results studies on roles miRNAs...

The increased use of radiation-based medical imaging methods such as computer tomography is a matter concern due to potential radiation-induced adverse effects. Efficient protection against detrimental effects has not been possible inadequate understanding alterations in signaling pathways. aim this study was elucidate the molecular mechanisms behind learning and memory deficits after acute low moderate doses ionizing radiation. Female C57BL/6J mice were irradiated on postnatal day 10...

MicroRNA (miRNA)-mediated regulation of the cellular transcriptome is an important epigenetic mechanism for fine-tuning regulatory pathways. These include processes related to skin cancer development, progression and metastasis. However, little known about role microRNA as intermediary in carcinogenic following exposure UV-radiation. We now show that UV irradiation human primary keratinocytes modulates expression several miRNAs. A common set miRNAs was influenced by both UVA UVB. each...

Patients suffering from brain malignancies are treated with high-dose ionising radiation. However, this may lead to severe learning and memory impairment. Preventive treatments minimise these side effects have not been possible due the lack of knowledge involved signalling pathways molecular targets. Mouse hippocampal neuronal HT22 cells were irradiated acute gamma doses 0.5 Gy, 1.0 Gy 4.0 Gy. Changes in cellular proteome investigated by isotope-coded protein label technology tandem mass...

Pancreatic cancer has a poor prognosis. New treatment options are urgently required to improve patient outcomes. One promising new class of anticancer drugs synthetic histone deacetylase inhibitors (HDACi) which modulate chromatin structure and gene expression by blocking deacetylation. In this study, we aimed at comparing the in vitro capacities HDACi SAHA CUDC-101 increase radiosensitivity human pancreatic tumor cell lines. Therefore, three lines (Su.86.86, MIA Paca-2, T3M-4) were treated...

Generated by Quaking (QKI), circular RNAs (circRNAs) are newly recognised non-coding RNA (ncRNA) members characterised tissue specificity, increased stability and enrichment within exosomes. Studies have shown that ionizing radiation (IR) can influence ncRNA transcription. However, it is unknown whether circRNAs or indeed QKI regulated IR. Microarray circRNA profiling next generation sequencing revealed expression was altered low medium dose exposure sourced predominantly from genes...

The circulatory system distributes nutrients, signaling molecules, and immune cells to vital organs soft tissues. Epidemiological, animal, in vitro cellular mechanistic studies have highlighted that exposure ionizing radiation (IR) can induce molecular changes subcellular milieus leading long-term health impacts, particularly on the system. Although mechanisms for pathologies are not fully elucidated, endothelial dysfunction is proven be a critical event via radiation-induced oxidative...

Journal Article The WST survival assay: an easy and reliable method to screen radiation-sensitive individuals Get access A. Guertler, Guertler 1Department SG Radiation Protection Health, Federal Office for Protection, Oberschleissheim, Germany Search other works by this author on: Oxford Academic PubMed Google Scholar Kraemer, Kraemer 2Institute of Biology, Helmholtz Zentrum Munich—German Research Center Environmental Neuherberg, U. Roessler, Roessler S. Hornhardt, Hornhardt Kulka, Kulka...

Abstract: Radiotherapy (RT) is a widely used and effective non-surgical cancer treatment. However, the mechanism behind several major challenges to efficacy, such as tumor radioresistance, normal tissue toxicity, individual radiation hypersensitivity promoted metastatic spread, are not fully understood. The involvement of microRNAs (miRNAs) in processes important for responses, including DNA damage repair, activation signalling cascades or changes microenvironment, being recognized. Most...

Normal tissue toxicity is a dose-limiting factor in radiation therapy. Therefore, detailed understanding of the normal response to necessary predict risk and development strategies for protection. One component that continuously exposed during therapeutic irradiation circulating population peripheral blood mononuclear cells (PBMC). PBMCs are highly sensitive ionizing (IR); however, little known about how IR affects PBMC on systemic level. It was aim this study investigate whether capable...

Increased levels of the chaperone protein GRP78 have been implicated in poorer outcomes cancer therapy. We therefore explored functional connection between expression and development radioresistance metastatic behavior HNSCC.The association gene survival HNSCC patients was examined using TCGA database. The influence ionizing radiation on cell lines, their secreted extracellular vesicles (EV) non-irradiated EV-recipient cells investigated by Western Blot FACS. consequences chemical inhibition...

Purpose: Epidemiological studies indicate that radiation doses as low 0.5 Gy increase the risk of cardiovascular disease decades after exposure. The aim present study was to investigate whether this dose causes late molecular alterations in endothelial cells could support population-based data.Materials and methods: Human coronary artery were irradiated at (X-ray) radiation-induced changes proteome investigated different time intervals (1, 7 14 d) using ICPL technology. Key identified by...