A5081328429- Effects of Radiation Exposure
- Mitochondrial Function and Pathology
- Radiation Therapy and Dosimetry
- Cancer, Hypoxia, and Metabolism
- Chemotherapy-induced cardiotoxicity and mitigation
- Radiation Dose and Imaging
- Advanced Proteomics Techniques and Applications
- Cardiac Imaging and Diagnostics
- Extracellular vesicles in disease
- Redox biology and oxidative stress
- Cardiovascular Disease and Adiposity
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Telomeres, Telomerase, and Senescence
- Connexins and lens biology
- Heat shock proteins research
- Circular RNAs in diseases
- Mass Spectrometry Techniques and Applications
- interferon and immune responses
- Metabolomics and Mass Spectrometry Studies
- Glutathione Transferases and Polymorphisms
- Bioinformatics and Genomic Networks
- Cancer-related molecular mechanisms research
- Neurogenesis and neuroplasticity mechanisms
- Ubiquitin and proteasome pathways
Federal Office for Radiation Protection
2021-2025
Helmholtz Zentrum München
2013-2022
Center for Environmental Health
2015
German Center for Infection Research
2010
Philipps University of Marburg
2007
Backround Radiation therapy treatment of breast cancer, Hodgkin's disease or childhood cancers expose the heart to high local radiation doses, causing an increased risk cardiovascular in survivors decades after treatment. The mechanisms that underlie damage remain poorly understood so far. Previous data show impairment mitochondrial oxidative metabolism is directly linked development disease. Methodology/Principal findings In this study, radiation-induced vivo effects on cardiac proteome and...
Radiation is a highly efficient therapy in squamous head and neck carcinoma (HNSCC) treatment. However, local recurrence metastasis are common complications. Recent evidence shows that cancer-cell-derived exosomes modify tumour cell movement metastasis. In this study, we link radiation-induced changes of to their ability promote migration recipient HNSCC cells. We demonstrate isolated from irradiated donor cells boost the motility BHY FaDu. Molecular data identified enhanced AKT-signalling,...
Chronic low-dose ionizing radiation induces cardiovascular disease in human populations but the mechanism is largely unknown. We suggested that chronic exposure may induce endothelial cell senescence associated with vascular damage vivo. investigated whether causing a change onset of cells vitro. Indeed, when exposed to continuous rate gamma (4.1 mGy/h), primary umbilical vein (HUVECs) initiated much earlier than nonirradiated control cells. changes protein expression HUVECs before and...
Epidemiological data from radiotherapy patients show the damaging effect of ionizing radiation on heart and vasculature. The endothelium is main target damage contributes essentially to development cardiac injury. However, molecular mechanisms behind radiation-induced endothelial dysfunction are not fully understood. In present study, 10-week-old C57Bl/6 mice received local X-ray doses 8 or 16 Gy were sacrificed after weeks; controls sham-irradiated. microvascular cells isolated tissue using...
Accidental nuclear scenarios lead to environmental contamination of unknown level. Immediate radiation-induced biological responses that trigger processes leading adverse health effects decades later are not well understood. A comprehensive proteomic analysis provides a promising means identify and quantify the initial damage after radiation exposure. Early changes in cardiac tissue C57BL/6 mice exposed total body irradiation were studied, using dose relevant both intentional accidental...
The etiology of radiation-induced cardiovascular disease (CVD) after chronic exposure to low doses ionizing radiation is only marginally understood. We have previously shown that a low-dose rate (4.1 mGy/h) causes human umbilical vein endothelial cells (HUVECs) prematurely senesce. now show dose 2.4 mGy/h also able trigger premature senescence in HUVECs, primarily indicated by loss growth potential and the appearance senescence-associated markers ß-galactosidase (SA-ß-gal) p21. In contrast,...
Formalin-fixed paraffin-embedded (FFPE) tissue has recently gained interest as an alternative to fresh/frozen for retrospective protein biomarker discovery. However, during the fixation process, proteins undergo degradation and cross-linking, making conventional analysis technologies problematic. In this study, we have compared several extraction separation methods of in FFPE tissues. Incubation sections at high temperature with a novel buffer (20 mM Tris-HCl, pH 8.8, 2% SDS, 1%...
Multiple cell types secrete exosome-like extracellular vesicles (ELVs) to the environment. Pathological conditions can produce characteristic changes vesicle cargo. We investigated if ionizing radiation is capable of inducing in protein and microRNA (miRNA) cargo ELVs.Whole blood samples from healthy donors were irradiated with 2 Gy gamma rays then peripheral mononuclear cells plasma separated residual co-cultivated for 24 h. The released ELVs collected by differential ultracentrifugation...
Extracellular vesicles (EVs) are cell-derived membrane-bound organelles that have generated interest as they reflect the physiological condition of their source. Mass spectrometric (MS) analyses protein cargo EVs may lead to discovery biomarkers for diseases. However, a comprehensive MS-based proteomics analysis, an optimal lysis is required. Six methods extraction from secreted by head and neck cell line BHY were compared. Commercial radioimmunoprecipitation assay (RIPA) buffer outperformed...
Radiation exposure of the thorax is associated with a markedly increased risk cardiac morbidity and mortality latency period decades. Although many studies have confirmed damaging effect ionizing radiation on myocardium endothelial structure function, molecular mechanism behind this damage not yet elucidated. Peroxisome proliferator-activated receptor alpha (PPAR alpha), transcriptional regulator lipid metabolism in heart tissue, has recently received great attention development...
Therapeutic irradiation of pediatric and adult patients can profoundly affect neurogenesis, cognitive impairment manifests as a deficit in hippocampal-dependent functions. Age plays major role susceptibility to radiation, younger children are at higher risk decay when compared adults. Cranial affects hippocampal neurogenesis by induction DNA damage neural progenitors, through the disruption neurogenic microenvironment, defective integration newborn neurons into neuronal network. Our goal...
// Omid Azimzadeh 1 , Tamara Azizova 2 Juliane Merl-Pham 3 Vikram Subramanian Mayur V. Bakshi Maria Moseeva Olga Zubkova Stefanie M. Hauck Nataša Anastasov Michael J. Atkinson 1, 4 Soile Tapio Helmholtz Zentrum München-German Research Center for Environmental Health (GmbH), Institute of Radiation Biology, Neuherberg, Germany Southern Urals Biophysics Institute, Russian Federation, Ozyorsk, Russia Unit Protein Science, Munich, Chair Technical University Correspondence to: Tapio, email:...
Abstract PARTICLE (Gene PARTICL - ‘ P romoter of MAT2A A ntisense R adia T ion I nduced C irculating L ncRNA) expression is transiently elevated following low dose irradiation typically encountered in the workplace and from natural sources. This long non-coding RNA recruits epigenetic silencers for cis -acting repression its neighbouring Methionine adenosyltransferase 2A gene. It now emerges that operates as a trans-acting mediator DNA histone lysine methylation. Chromatin...
Epidemiological data from patients undergoing radiotherapy for thoracic tumors clearly show the damaging effect of ionizing radiation on cardiovascular system. The long-term impairment heart function and structure after local high-dose irradiation is associated with systemic inflammatory response, contraction impairment, microvascular damage, cardiac fibrosis. goal present study was to investigate molecular mechanisms involved in this process. C57BL/6J mice received a single X-ray dose 16 Gy...
Because of the increasing application ionizing radiation in medicine, quantitative data on effects low-dose are needed to optimize protection, particularly with respect cataract development. Using mice as mammalian animal model, we applied a single dose 0, 0.063, 0.125 and 0.5 Gy at 10 weeks age, determined lens opacities for up 2 years compared it overall survival, cytogenetic alterations cancer The highest was significantly associated increased body weight reduced survival rate....