A5075415433- Mitochondrial Function and Pathology
- Cell Adhesion Molecules Research
- Infectious Diseases and Mycology
- Mycobacterium research and diagnosis
- Chemotherapy-induced cardiotoxicity and mitigation
- Tuberculosis Research and Epidemiology
- Anesthesia and Neurotoxicity Research
- ATP Synthase and ATPases Research
- Mosquito-borne diseases and control
- Trace Elements in Health
- Effects of Radiation Exposure
- Cancer-related cognitive impairment studies
- Cannabis and Cannabinoid Research
- Ocular Disorders and Treatments
- Sarcoma Diagnosis and Treatment
- bioluminescence and chemiluminescence research
- Developmental Biology and Gene Regulation
- Genetics, Aging, and Longevity in Model Organisms
- Dermatological diseases and infestations
- Gene Regulatory Network Analysis
- interferon and immune responses
- Neurogenesis and neuroplasticity mechanisms
- Cardiac Imaging and Diagnostics
- Veterinary Oncology Research
- Cellular Mechanics and Interactions
Helmholtz Zentrum München
2012-2021
Ludwig-Maximilians-Universität München
2009-2012
In Wilson disease (WD), functional loss of ATPase copper-transporting β (ATP7B) impairs biliary copper excretion, leading to excessive accumulation in the liver and fulminant hepatitis. Current US Food Drug Administration- European Medicines Agency-approved pharmacological treatments usually fail restore homeostasis patients with WD who have progressed acute failure, leaving transplantation as only viable treatment option. Here, we investigated therapeutic utility methanobactin (MB), a...
Dietary restriction regimes extend lifespan in various animal models. Here we show that longevity male C57BL/6J mice subjected to every-other-day feeding is associated with a delayed onset of neoplastic disease naturally limits these animals. We compare more than 200 phenotypes over 20 tissues aged animals fed lifelong or ad libitum access food diet determine whether molecular, cellular, physiological and histopathological aging features develop slowly controls. also analyze the effects on...
Epidemiological data from radiotherapy patients show the damaging effect of ionizing radiation on heart and vasculature. The endothelium is main target damage contributes essentially to development cardiac injury. However, molecular mechanisms behind radiation-induced endothelial dysfunction are not fully understood. In present study, 10-week-old C57Bl/6 mice received local X-ray doses 8 or 16 Gy were sacrificed after weeks; controls sham-irradiated. microvascular cells isolated tissue using...
The basement membrane is important for proper tissue development, stability, and physiology. Major components of the include laminins type IV collagens. procollagens Col4a1 Col4a2 form heterotrimer [alpha1(IV)]2[alpha2(IV)], which ubiquitously expressed in membranes during early developmental stages. We present genetic, molecular, phenotypic characterization nine three missense mutations recovered random mutagenesis experiments mouse. Heterozygous carriers express defects eye, brain, kidney...
Epidemiological evidence suggests that low doses of ionising radiation (≤1.0 Gy) produce persistent alterations in cognition if the exposure occurs at a young age. The mechanisms underlying such are unknown. We investigated long-term effects total body gamma on neonatally exposed NMRI mice molecular and cellular level to elucidate neurodegeneration. Significant spontaneous behaviour were observed 2 4 months following single 0.5 or 1.0 Gy exposure. Alterations brain proteome, transcriptome,...
ABSTRACT Vector-borne flaviviruses, such as tick-borne encephalitis virus (TBEV), West Nile virus, and dengue cause millions of infections in humans. TBEV causes a broad range pathological symptoms, ranging from meningitis to severe or even hemorrhagic fever, with high mortality. Despite the availability an effective vaccine, incidence is increasing. Not much known about role innate immune system control infections. Here, we show that type I interferon (IFN) essential for protection against...
Glutathione peroxidase 4 (GPX4) is unique as it the only enzyme that can prevent detrimental lipid peroxidation in vivo by reducing peroxides to respective alcohols thereby stabilizing oxidation products of unsaturated fatty acids. During reticulocyte maturation, mediated 15-lipoxygenase humans and rabbits 12/15-lipoxygenase (ALOX15) mice was considered initiating event for elimination mitochondria but now known occur through mitophagy. Yet, genetic ablation Alox15 gene failed provide...
Animal welfare requires the adequate housing of animals to ensure health and well-being. The application environmental enrichment is a way improve well-being laboratory animals. However, it important know whether these items can be incorporated in experimental mouse husbandry without creating divide between past future results. Previous small-scale studies have been inconsistent throughout literature, not yet completely understood how might endanger comparability results scientific...
Radiation exposure of the thorax is associated with a markedly increased risk cardiac morbidity and mortality latency period decades. Although many studies have confirmed damaging effect ionizing radiation on myocardium endothelial structure function, molecular mechanism behind this damage not yet elucidated. Peroxisome proliferator-activated receptor alpha (PPAR alpha), transcriptional regulator lipid metabolism in heart tissue, has recently received great attention development...
The formation of amyloid fibrils by human islet polypeptide protein (hIAPP) has been implicated in pancreas dysfunction and diabetes. However, efficient treatment options to reduce vivo are still lacking. Therefore, we tested the effect epigallocatechin gallate (EGCG) on fibril vitro vivo. To determine binding hIAPP EGCG, interaction studies were performed. inhibit plaque vivo, homozygous (tg/tg), hemizygous (wt/tg), control mice (wt/wt) treated with EGCG. EGCG bound induced amorphous...
// Stefan J. Kempf 1, 2 , Dirk Janik 3 Zarko Barjaktarovic 1 Ignacia Braga-Tanaka III 4 Satoshi Tanaka Frauke Neff Anna Saran 5 Martin R. Larsen Soile Tapio Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Neuherberg, Germany Department Biochemistry and Molecular University Southern Denmark, Odense, Denmark Pathology, Sciences, Rokkasho, Japan Laboratory Biomedical Technologies, Agenzia Nazionale per le Nuove Tecnologie,...
Morphological lesions in kidneys and brain are all too often considered diagnostic for confirmation of encephalitozoonosis rabbits. The current study evaluated the value histology versus other etiological tests, including immunohistochemistry real-time polymerase chain reaction (PCR) Encephalitozoon cuniculi infection diagnosis. Samples brain, heart, lungs, intestine, liver, from 81 rabbits were examined morphological attributed to E. as well presence spores antigen. Of these, 55 tested DNA....
Under the label of German Mouse Clinic (GMC), a concept has been developed and implemented that allows better understanding human diseases on pathophysiological molecular level. This includes crosstalk between different organs, pleiotropy genes, systemic impact envirotypes drugs. In GMC, experts from various fields mouse genetics physiology, in close collaboration with clinicians, work side by under one roof. The GMC is an open-access platform for scientific community providing phenotypic...
Recently, mutations in the mitochondrial translation optimization factor 1 gene (MTO1) were identified as causative children with hypertrophic cardiomyopathy, lactic acidosis and respiratory chain defect. Here, we describe an MTO1-deficient mouse model generated by trap mutagenesis that mirrors human phenotype remarkably well. As patients, most prominent signs symptoms cardiovascular included bradycardia cardiomyopathy. In addition, mutant mice showed a marked worsening of arrhythmias during...
Ewing's sarcoma (ES) is the second most common bone-associated malignancy in children and driven by fusion oncogene EWS/FLI1 characterised rapid growth early metastasis. Here, we explored role of Zyxin-related protein thyroid receptor interacting 6 (TRIP6) ES. The Zyxin family comprises seven homologous proteins involved migration proliferation many cell types which has been described as a tumour suppressor ES.By interrogation published microarray data (n = 1254), observed that all proteins,...
Recent epidemiological data indicate that radiation doses as low those used in computer tomography may result long-term neurocognitive side effects. The aim of this study was to elucidate molecular alterations related memory formation the brain after and moderate γ radiation. Female C57BL/6J mice were irradiated on postnatal day 10 with total body 0.1, 0.5, or 2.0 Gy; control group sham-irradiated. proteome analysis hippocampus, cortex, synaptosomes isolated from these regions indicated...
A juvenile dwarf rabbit ( Oryctolagus cuniculus) with clinical signs of dyspnea and suspected ascites was submitted for necropsy. The main macroscopic findings were a watery red pleural effusion some whitish striated foci in the lungs. In addition, there multifocal scars cortex kidneys. histologic examination lungs showed severe granulomatous pneumonia detection acid-fast bacilli, kidneys, an interstitial chronic lymphoplasmacellular nephritis fibrosis, brain, partly necrotizing encephalitis...
Abstract Pathogenic variants in the WDR45 (OMIM: 300,526) gene on chromosome Xp11 are genetic cause of a rare neurological disorder characterized by increased iron deposition basal ganglia. As encodes beta-propeller scaffold protein with putative role autophagy, disease has been named Beta-Propeller Protein-Associated Neurodegeneration (BPAN). BPAN represents one four most common forms Brain Iron Accumulation (NBIA). In current study, we generated and whole-body Wdr45 knock-out (KO) mouse...
In the mouse Pax6 function is critical in a dose-dependent manner for proper eye development. contiguous gene deletions were shown to be homozygous lethal at an early embryonic stage. Heterozygotes express belly spotting and extreme microphthalmia. The phenotype more severe than heterozygous intragenic null mutants, raising possibility that are functionally different from mutations or region distinct included affects phenotype. We recovered identified exact regions deleted three new...