A5085816723- Chronic Obstructive Pulmonary Disease (COPD) Research
- Neonatal Respiratory Health Research
- Medical Imaging Techniques and Applications
- Advanced X-ray Imaging Techniques
- Calibration and Measurement Techniques
- Virus-based gene therapy research
- Advanced X-ray and CT Imaging
- Cancer-related gene regulation
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Pulmonary Hypertension Research and Treatments
- Epigenetics and DNA Methylation
- Congenital Diaphragmatic Hernia Studies
- Pediatric health and respiratory diseases
- Parasites and Host Interactions
- Single-cell and spatial transcriptomics
- Nuclear Structure and Function
- Advanced Proteomics Techniques and Applications
- Immune cells in cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced MRI Techniques and Applications
- Atomic and Subatomic Physics Research
- Genomics and Chromatin Dynamics
Helmholtz Zentrum München
2010-2017
Ludwig-Maximilians-Universität München
2008-2015
German Center for Lung Research
2013-2015
Freie Universität Berlin
2008
University of Regensburg
2004
In early stages of various pulmonary diseases, such as emphysema and fibrosis, the change in X-ray attenuation is not detectable with absorption-based radiography. To monitor morphological changes that alveoli network undergoes progression these we propose using dark-field signal, which related to small-angle scattering sample. Combined image, signal enables better discrimination between healthy emphysematous lung tissue a mouse model. All measurements have been performed at 36 keV...
Chronic obstructive pulmonary disease (COPD) is a devastating disease, for which no causal therapy available.To characterize WNT/β-catenin signaling in COPD humans and elucidate its potential role as preventive therapeutic target experimental emphysema mice.The expression, localization, activity of was assessed 12 transplant donor samples using quantitative reverse transcriptase polymerase chain reaction, immunohistochemistry, Western blotting. The elastase- cigarette smoke-induced...
To test the hypothesis that joint distribution of x-ray transmission and dark-field signals obtained with a compact cone-beam preclinical scanner polychromatic source can be used to diagnose pulmonary emphysema in ex vivo murine lungs.The animal care committee approved this study. Three excised lungs three control were imaged by using grating-based micro-computed tomographic (CT) scanner. evaluate diagnostic value, natural logarithm relative scatter signal plotted on per-pixel basis...
Chronic obstructive lung disease determines morbidity and mortality of patients with cystic fibrosis (CF). CF airways are characterized by a nonresolving neutrophilic inflammation. After pathogen contact or prolonged activation, neutrophils release DNA fibres decorated antimicrobial proteins, forming neutrophil extracellular traps (NETs). NETs have been described to act in beneficial way for innate host defense bactericidal, fungicidal, virucidal actions. On the other hand, excessive NET...
Purpose To assess whether grating-based X-ray dark-field imaging can increase the sensitivity of projection images in diagnosis pulmonary emphysema and allow for a more accurate assessment distribution. Materials Methods Lungs from three mice with healthy were imaged ex vivo using laser-driven compact synchrotron source. Median signal intensities transmission (T), (V) combined parameter (normalized scatter) compared between control group. determine diagnostic value each differentiating...
The chitinase-like protein YKL-40 was found to be increased in patients with severe asthma and chronic obstructive pulmonary disease (COPD), two conditions featuring neutrophilic infiltrates. Based on these studies a previous report indicating that neutrophils secrete YKL-40, we hypothesized plays key role cystic fibrosis (CF) lung disease, prototypic disease. aim of this study (i) analyze levels human murine CF (ii) investigate whether single-nucleotide polymorphisms (SNPs) modulate...
Under the label of German Mouse Clinic (GMC), a concept has been developed and implemented that allows better understanding human diseases on pathophysiological molecular level. This includes crosstalk between different organs, pleiotropy genes, systemic impact envirotypes drugs. In GMC, experts from various fields mouse genetics physiology, in close collaboration with clinicians, work side by under one roof. The GMC is an open-access platform for scientific community providing phenotypic...
Nuclear myosin I (NM1) is a nuclear isoform of the well-known "cytoplasmic" Myosin 1c protein (Myo1c). Located on 11(th) chromosome in mice, NM1 results from an alternative start transcription Myo1c gene adding extra 16 amino acids at N-terminus. Previous studies revealed its roles RNA Polymerase and II transcription, chromatin remodeling, chromosomal movements. Its localization signal localized middle molecule therefore directs both isoforms to nucleus.In order trace specific functions...
The nuclei of most vertebrate cells contain members the high mobility group N (HMGN) protein family, which bind specifically to nucleosome core particles and affect chromatin structure function, including transcription. Here, we study biological role this family by systematic analysis phenotypes tissue transcription profiles in mice lacking functional HMGN variants. Phenotypic Hmgn1(tm1/tm1), Hmgn3(tm1/tm1), Hmgn5(tm1/tm1) their wild type littermates with a battery standardized tests...
Tumor necrosis factor-α (TNFα) is known to inhibit renin gene expression in juxtaglomerular cells, which are the main source of vivo. In present study we aimed characterize intracellular mechanisms TNFα signaling mouse cell line As4.1. was found activate NFκB, one principal mediators signal transduction. Constitutive activation NFκB suppressed transcription, but appeared not target binding sites promoter. Thus, canonical sequences promoter, seemed be involved suppression transcription by...
Abstract Background Supercoiled topology of transfected plasmid DNA (pDNA) is critical for transgene expression in mammalian cells. In the present study, we analysed supercoiled pDNA concatemers. Methods Jurkat T cells were with a 4.7‐kb monomeric and, parallel, 9.4‐kb dimeric pEGFP concatemer using electroporation. The absolute amounts delivered into cytoplasm and nucleus quantified by quantitative real‐time polymerase chain reaction. Further, number mean fluorescent intensity (MFI)...
An experimental comparison of the contrast-to-noise ratio (CNR) between transmission and dark-field signals in grating-based X-ray imaging for ex-vivo murine lung tissue.Lungs from three healthy mice were imaged ex vivo using a laser-driven compact synchrotron source. Background noise signal was quantified by measuring standard deviation region interest (ROI) placed homogeneous area outside specimen. Image contrast range rectangular ROIs central peripheral parenchyma. The relative gain (RCG)...
The transcription factor Zscan10 had been attributed a role as pluripotency in embryonic stem cells based on its interaction with Oct4 and Sox2 vitro assays. Here we suggest potential of controlling progenitor cell populations vivo. Mice homozygous for mutation exhibit reduced weight, mild hypoplasia the spleen, heart long bones phenocopy an eye malformation previously described hypomorphs. Phenotypic abnormalities are supported by nature expression midgestation embryos adults suggesting...
Chronic obstructive pulmonary disease (COPD) is characterized by an irreversible loss of lung function and one the most prevalent severe diseases worldwide. A major feature COPD emphysema, which progressive alveolar tissue. Coactivator-associated arginine methyltransferase-1 (CARM1) regulates histone methylation transcription genes involved in senescence, proliferation, differentiation. Complete CARM1 leads to disrupted differentiation maturation epithelial type II (ATII) cells. We thus...
Genomic integration by the Streptomyces bacteriophage ϕC31 integrase is a promising tool for non-viral gene therapy of various genetic disorders. We investigated recombination activity in T cell derived lines, primary lymphocytes and CD34 + haematopoietic stem cells comparison to mesenchymal lines from lung-, liver- cervix-tissue. In enhanced long-term expression above control was observed only with high amounts mRNA. Transfections plasmids were not capable mediating transgene lines....
Several barriers have to be overcome in order achieve gene expression target cells, e.g. cellular uptake, endosomal release and translocation the nucleus. Nuclear localization sequences (NLS) enhance delivery by increasing uptake of plasmid DNA (pDNA) So far, only monopartite NLS were analysed for non-viral delivery. In this study, we examined characteristics a novel bipartite like construct, namely Ku70. We synthesized dimeric structure modified from Ku70 protein (Ku702-NLS), nuclear...
Pulmonary emphysema is a widespread disorder characterized by irreversible destruction of alveolar walls. The spatial distribution the disease, so far, could only be obtained using an X-ray CT scan, implying high patient dose. scattering on structures measured in dark-field signal. signal dependent size alveoli and therefore, combination absorption darkfield explored for mapping lung x-ray projection images. In this study three excised murine lungs with pulmonary control samples were imaged...
Purpose To test the hypothesis that joint distribution of x-ray transmission and dark-field signals obtained with a compact cone-beam preclinical scanner polychromatic source can be used to diagnose pulmonary emphysema in ex vivo murine lungs. Materials Methods The animal care committee approved this study. Three excised lungs three control were imaged by using grating-based micro–computed tomographic (CT) scanner. evaluate diagnostic value, natural logarithm relative scatter signal plotted...
To assess whether grating-based X-ray dark-field imaging can increase the sensitivity of projection images in diagnosis pulmonary emphysema and allow for a more accurate assessment distribution. Lungs from three mice with healthy were imaged ex vivo using laser-driven compact synchrotron source. Median signal intensities transmission (T), (V) combined parameter (normalized scatter) compared between control group. determine diagnostic value each differentiating emphysematous lung tissue,...