A5075146821- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Mitochondrial Function and Pathology
- Molecular Biology Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Advanced Fluorescence Microscopy Techniques
- Animal Genetics and Reproduction
- Cancer, Hypoxia, and Metabolism
- ATP Synthase and ATPases Research
- Sperm and Testicular Function
- Advanced Electron Microscopy Techniques and Applications
- Cellular transport and secretion
- RNA Interference and Gene Delivery
- Gas Sensing Nanomaterials and Sensors
- Plasma Applications and Diagnostics
- Electron and X-Ray Spectroscopy Techniques
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
Czech Academy of Sciences, Institute of Molecular Genetics
2015-2024
Czech Academy of Sciences
2015-2024
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2023
University of Chemistry and Technology, Prague
2017-2021
Institute of Molecular Genetics
2010-2019
Czech Academy of Sciences, Institute of Physics
2018
Česká Zbrojovka (Czechia)
2017
Yerevan State University
2017
Genetikum
2015
Czech Academy of Sciences, Institute of Experimental Medicine
1998-2009
Recently, we showed that generation of tumours in syngeneic mice by cells devoid mitochondrial (mt) DNA (ρ0 cells) is linked to the acquisition host mtDNA. However, mechanism mtDNA movement between remains unresolved. To determine whether transfer involves whole mitochondria, injected B16ρ0 mouse melanoma into C57BL/6Nsu9-DsRed2 express red fluorescent protein their mitochondria. We document acquired mitochondria from animal, leading normalisation respiration. Additionally, knockdown key...
ABSTRACT The nuclear lamina forms a protein mesh that underlies the membrane. In most mammalian cells it contains intermediate filament proteins, lamins A, B and C. As their name indicates, are generally thought to be confined periphery. We now show they also form part of diffuse skeleton ramifies throughout interior nucleus. Unlike peripheral counterparts, these internal buried in dense chromatin so inaccessible antibodies, but accessibility can increased by removing chromatin. Knobs nodes...
A nuclear isoform of myosin I β that contains a unique 16–amino acid amino-terminal extension has been identified. An affinity-purified antibody to the peptide demonstrated staining. Confocal and electron microscopy revealed colocalized with RNA polymerase II in an α-amanitin– actinomycin D–sensitive manner. The coimmunoprecipitated blocked vitro synthesis. This appears be complex may affect transcription.
ABSTRACT Sites of transcription ribosomal RNA in HeLa cells were visualized by electron microscopy. Cells either incubated with Br-uridine, or permeabilized and then BrUTP, before sites containing Br-RNA immunolabelled gold particles. Short incuba-tions ensured that most incorporated analogue remained at synthetic sites. Fibrillar centres unlabelled except their periphery; label was concentrated over certain regions the surrounding dense fibrillar component. These results suggest component...
Expression of the HER2 oncogene in breast cancer is associated with resistance to treatment, and Her2 may regulate bioenergetics. Therefore, we investigated whether disruption electron transport chain (ETC) a viable strategy eliminate Her2high disease.We demonstrate that cells tumors have increased assembly respiratory supercomplexes (SCs) complex I-driven respiration vitro vivo. They are also highly sensitive MitoTam, novel mitochondrial-targeted derivative tamoxifen. Unlike tamoxifen,...
ABSTRACT Sites of replication in synchronized HeLa cells were visualized by light and electron microscopy; perme-abilized incubated with biotin-16-dUTP, incorporation sites immunolabelled. Electron microscopy thick resinless sections from which ∼90% chromatin had been removed showed that most DNA synthesis occurs specific dense structures (replication factories) attached to a diffuse nucleoskeleton. These factories appear at the end G1-phase quickly become active; as S-phase progresses, they...
ABSTRACT Electron and confocal microscopy were used to observe the entry movement of polyomavirus virions artificial virus-like particles (VP1 pseudocapsids) in mouse fibroblasts epithelial cells. No visible differences adsorption internalization VP1 pseudocapsids (“empty” or containing DNA) observed. Viral entered cells internalized smooth monopinocytic vesicles, often proximity larger, caveola-like invaginations. Both “empty” vesicles derived from caveolae viral stained with...
Abstract Cell growth and survival depend on a delicate balance between energy production synthesis of metabolites. Here, we provide evidence that an alternative mitochondrial complex II (CII) assembly, designated as CII low , serves checkpoint for metabolite biosynthesis under bioenergetic stress, with cells suppressing their utilization by modulating DNA cell cycle progression. Depletion leads to imbalance in synthesis, evidenced recovery the de novo pyrimidine pathway unlocking arrest from...
We produced and affinity-purified polyclonal antibodies to adrenal myosin I. These recognize I by Western blot analysis (116 kDa) inhibit the actin-activated ATPase activity of purified They also a 120-kDa protein in extracts prepared from many different cell lines. Fluorescence microscopy demonstrated presence immunoreactive material perinuclear region, leading edges, nuclei 3T3 cells. nuclear staining mouse oocytes at germinal vesicle stage pronuclei during fertilization. Confocal...
The pVI150 catabolic plasmid of Pseudomonas sp. strain CF600 carries the dmp system, which comprises divergently transcribed dmpR gene and operon coding for enzymes required growth on (methyl)phenols. constitutively expressed DmpR transcriptional activator positively controls expression RpoN-dependent promoter in presence aromatic effector medium. However, magnitude response differs depending position methyl substituent ring. Experiments involving an elevated copy number system demonstrate...
RNA polymerase I (Pol I) transcription is essential for the cell cycle, growth, and overall protein synthesis in eukaryotes. We found that phosphatidylinositol 4,5-bisphosphate (PIP2) a part of complex on active ribosomal promoter during transcription. PIP2 makes with Pol factor UBF nucleolus. depletion reduces which can be rescued by addition exogenous PIP2. In addition, also binds directly to pre-rRNA processing factor, fibrillarin (Fib), co-localizes nascent transcripts binding Fib...
The NtrC‐like regulator DmpR controls transcription from the dmp operon that encodes enzymes for catabolism of phenol and some related aromatic compounds. activates σ 54 ‐dependent ‐operon promoter in presence pathway substrates or structural analogues growth medium. Using affinity‐purified a truncated derivative, we show here compounds directly activate ATPase activity this protein vitro , amino‐terminal domain represses absence an ligand. In order to dissect activation process, derivatives...
Abstract γ‐Tubulin is assumed to be a typical cytosolic protein necessary for nucleation of microtubules from microtubule organizing centers. Using immunolocalization and cell fractionation techniques in combination with siRNAi expression FLAG‐tagged constructs, we have obtained evidence that γ‐tubulin also present nucleoli mammalian interphase cells diverse cellular origins. Immunoelectron microscopy has revealed localization outside fibrillar centers where transcription ribosomal DNA takes...
To maintain growth and division, cells require a large-scale production of rRNAs which occurs in the nucleolus. Recently, we have shown interaction nucleolar phosphatidylinositol 4,5-bisphosphate (PIP2) with proteins involved rRNA transcription processing, namely RNA polymerase I (Pol I), UBF, fibrillarin. Here extend study by investigating transcription-related localization PIP2 regards to processing complexes Pol I. achieve this, used either physiological inhibition during mitosis or...