A5051194123- Developmental Biology and Gene Regulation
- Hydrogen's biological and therapeutic effects
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Reproductive biology and impacts on aquatic species
- Vibrio bacteria research studies
- Anesthesia and Neurotoxicity Research
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- Birth, Development, and Health
- Adrenal and Paraganglionic Tumors
- Wound Healing and Treatments
- Genomics and Chromatin Dynamics
- Fecal contamination and water quality
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Planarian Biology and Electrostimulation
- Silk-based biomaterials and applications
- RNA modifications and cancer
- Physiological and biochemical adaptations
- Escherichia coli research studies
- Reproductive Biology and Fertility
Czech Academy of Sciences, Institute of Biotechnology
2018-2024
Czech Academy of Sciences
2021-2024
St. George's University
2016
Abstract Cell growth and survival depend on a delicate balance between energy production synthesis of metabolites. Here, we provide evidence that an alternative mitochondrial complex II (CII) assembly, designated as CII low , serves checkpoint for metabolite biosynthesis under bioenergetic stress, with cells suppressing their utilization by modulating DNA cell cycle progression. Depletion leads to imbalance in synthesis, evidenced recovery the de novo pyrimidine pathway unlocking arrest from...
Mitochondrial oxidative phosphorylation (OXPHOS) generates ATP, but OXPHOS also supports biosynthesis during proliferation. In contrast, the role of quiescence, beyond ATP production, is not well understood. Using mouse models inducible deficiency in all cell types or specifically vascular endothelium that negligibly relies on OXPHOS-derived we show selectively quiescence provides stress resistance by supporting macroautophagy/autophagy. Mechanistically, constitutively low levels endogenous...
The study of the mechanisms controlling wound healing is an attractive area within field biology, with it having a potentially significant impact on health sector given current medical burden associated in elderly population. Healing complex process and includes many steps that are regulated by coding noncoding RNAs, proteins other molecules. Nitric oxide (NO) one these small molecule regulators its function has already been inflammation angiogenesis during adult healing.Our results showed...
Asymmetric cell division is a ubiquitous feature during the development of higher organisms. Asymmetry achieved by differential localization or activities biological molecules such as proteins, and coding non-coding RNAs. Here, we present subcellular transcriptomic proteomic analyses along animal-vegetal axis Xenopus laevis eggs. More than 98% maternal mRNAs could be categorized into four profile groups: animal, vegetal, extremely newly described group that call which are enriched in animal...
DNA damage during early life stages may have a negative effect on embryo development, inducing mortality and malformations that long-lasting effects adult life. Therefore, in the current study, we analyzed of induced by genotoxicants (camptothecin (CPT) olaparib) at different development. The survival, fragmentation, transcriptome, proteome endangered sturgeon Acipenser ruthenus were analyzed. Sturgeons are non-model fish species can provide new insights into response transcriptomic...
Urinary Tract Infections (UTIs) are among one of the most common infections in women, with uropathogenic E. coli (UPEC) being involved 80% cases. In addition, exhibits an increasing resistance to broad spectrum antimicrobial agents as well subsequent generations these drugs. The genetic diversity and antibiotic patterns both clinical environmental isolates were studied predict potential transmission organisms genes for from three different major eco-habitats including gut iguanas’ fresh...
The oocyte is a unique cell, from which develops complex organism comprising of germ layers, tissues and organs. In some vertebrate species it known that the asymmetrical localization biomolecules within what drives spatial differentiation daughter cells required for embryogenesis. This asymmetry first established to produce an animal-vegetal (A-V) axis reflects future specification ectoderm, mesoderm, endoderm layers. Several pathways vegetal maternal transcripts have already been described...
The merit of RNASeq data relies heavily on correct normalization. However, most methods assume that the majority transcripts show no differential expression between conditions. This assumption may not always be correct, especially when one condition results in overexpression. We present a new method (NormQ) to normalize library size, using relative proportion observed from RT-qPCR selected marker genes. was compared against popular median-of-ratios method, simulated and real-datasets. NormQ...
The asymmetric localization of biomolecules is critical for body plan development. One the most popular model organisms early embryogenesis studies Xenopus laevis but there a lack information in other animal species. Here, we compared development two amphibian species-the frog X. and axolotl Ambystoma mexicanum. This study aimed to identify asymmetrically localized RNAs along animal-vegetal axis during A. For that purpose, performed spatial transcriptome-wide analysis at low resolution,...
Abstract DNA damage during early life stages may have a negative effect on embryo development, inducing malformations that long-lasting effects adult life. Therefore, in the current study, we analyzed of induced by genotoxicants (camptothecin (CPT) and olaparib) at different development. We survival, fragmentation, transcriptome, proteome endangered sturgeon Acipenser ruthenus . Sturgeons are non-model fish species can provide new insights into response The transcriptomic proteomic patterns...
Abstract Asymmetrical localization of biomolecules inside the egg, results in uneven cell division and establishment many biological processes, types body plan. However, our knowledge about evolutionary conservation localized mRNAs is still limited to a few candidates. Our goal was compare profiles along animal-vegetal axis mature eggs from four models, Xenopus laevis , Danio rerio Ambystoma mexicanum Acipenser ruthenus using spatial expression method called TOMO-Seq. We revealed that RNAs...