A5032719309- Mitochondrial Function and Pathology
- interferon and immune responses
- Cancer, Hypoxia, and Metabolism
- S100 Proteins and Annexins
- Immune cells in cancer
- Influenza Virus Research Studies
- Cancer Research and Treatments
- ATP Synthase and ATPases Research
- Cancer therapeutics and mechanisms
- Chromatin Remodeling and Cancer
- Single-cell and spatial transcriptomics
- Occupational and environmental lung diseases
- Synthesis and biological activity
- Lung Cancer Treatments and Mutations
- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- Cancer-related cognitive impairment studies
- Viral Infections and Outbreaks Research
- Cancer Cells and Metastasis
- T-cell and B-cell Immunology
- Diet and metabolism studies
- MicroRNA in disease regulation
- Chemotherapy-induced cardiotoxicity and mitigation
- Synthesis of β-Lactam Compounds
- Cancer-related Molecular Pathways
National University of Singapore
2013-2024
Duke-NUS Medical School
2022
Griffith University
2015-2019
National University Health System
2019
University of Southern Denmark
2019
National University Cancer Institute, Singapore
2019
The University of Texas at Austin
2005
Recently, we showed that generation of tumours in syngeneic mice by cells devoid mitochondrial (mt) DNA (ρ0 cells) is linked to the acquisition host mtDNA. However, mechanism mtDNA movement between remains unresolved. To determine whether transfer involves whole mitochondria, injected B16ρ0 mouse melanoma into C57BL/6Nsu9-DsRed2 express red fluorescent protein their mitochondria. We document acquired mitochondria from animal, leading normalisation respiration. Additionally, knockdown key...
Abstract Spatial transcriptomics technologies generate gene expression profiles with spatial context, requiring spatially informed analysis tools for three key tasks, clustering, multisample integration, and cell-type deconvolution. We present GraphST, a graph self-supervised contrastive learning method that fully exploits data to outperform existing methods. It combines neural networks learn informative discriminative spot representations by minimizing the embedding distance between...
Expression of the HER2 oncogene in breast cancer is associated with resistance to treatment, and Her2 may regulate bioenergetics. Therefore, we investigated whether disruption electron transport chain (ETC) a viable strategy eliminate Her2high disease.We demonstrate that cells tumors have increased assembly respiratory supercomplexes (SCs) complex I-driven respiration vitro vivo. They are also highly sensitive MitoTam, novel mitochondrial-targeted derivative tamoxifen. Unlike tamoxifen,...
Abstract Macrophages are potent immune cells with well-established roles in the response to stress, injury, infection and inflammation. The classically activated macrophages (M1) induced by lipopolysaccharide (LPS) express a wide range of pro-inflammatory genes. M2 T helper type 2 cytokines such as interleukin-4 (IL4) high levels anti-inflammatory tissue repair strong association between tumour well incidences leukocyte infiltration solid tumours have contributed discovery that...
Abstract Background Despite advancements in therapies, brain metastasis patients with triple negative subtype of breast cancer remains a therapeutic challenge. Activated microglia are often observed close proximity to, or within, malignant tumor masses, suggesting critical role that play progression. Annexin-A1 (ANXA1), glucocorticoid-regulated protein immune-regulatory properties, has been implicated the growth and many cancers. Its cancer-microglia signaling crosstalk is not known. Methods...
Background: Anthracyclines such as doxorubicin remain a primary treatment for hematological malignancies and breast cancers. However, cardiotoxicity induced by anthracyclines, possibly leading to heart failure, severely limits their application. The pathological mechanisms of anthracycline-induced cardiac injury are believed involve iron-overload-mediated formation reactive oxygen species (ROS), mitochondrial dysfunction, inflammation. dietary thione, ergothioneine (ET), is avidly absorbed...
Engagement of the mitochondrial-death amplification pathway is an essential component in chemotherapeutic execution cancer cells. Therefore, identification mitochondria-targeting agents has become attractive avenue for novel drug discovery. Here, we report anticancer activity a Osmium-based organometallic compound (hereafter named Os) on different colorectal carcinoma cell lines. HCT116 line was highly sensitive to Os and displayed characteristic features autophagy apoptosis; however,...
Abstract Within the last century, millions of lives have been lost to four major Influenza pandemics. These influenza pandemics were all caused by Type A viruses (IAV) through their ability undergo antigenic drifts and shifts. greater understanding IAV host–pathogen interactions is required develop effective therapeutics against future outbreaks. Annexin A1 (ANXA1) a phospholipid binding, calcium-dependent protein known play essential roles in multiple cellular functions including...
Malignant mesothelioma (MM) is an aggressive type of tumour causing high mortality. One reason for this paradigm may be the existence a subpopulation tumour-initiating cells (TICs) that endow MM with drug resistance and recurrence. The objective study was to identify characterise TIC in cells, using spheroid cultures, mesospheres, as model TICs. Mesospheres, typified by stemness markers CD24, ABCG2 OCT4, initiated tumours immunodeficient mice more efficiently than adherent cells. CD24...
Intracellular recognition of self and non-self -nucleic acids can result in the initiation effective pro-inflammatory anti-tumorigenic responses. We hypothesized that macrophages be activated by tumor-derived nucleic to induce inflammasome activation tumor microenvironment. show conditioned media (CM) IL-1β production, indicative primed macrophages. This could partially dependent on caspase 1/11, AIM2 NLRP3. enhances cell proliferation, migration invasion while coculture cells with...
Influenza viruses have been shown to use autophagy for their survival. However, the proteins and mechanisms involved in autophagic process triggered by influenza virus are unclear. Annexin-A1 (ANXA1) is an immunomodulatory protein regulation of immune response A (IAV) replication. In this study, using clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 (CRISPR associated 9) deletion ANXA1, combined with next-generation sequencing, we systematically analyzed critical role...
Background: High emotional or psychophysical stress levels have been correlated with an increased risk and progression of various diseases.How impacts the gut microbiota to influence metabolism subsequent cancer is unclear.Methods: Feces serum samples from BALB/c ANXA1 +/+ -/-mice without chronic restraint were used for 16S rRNA gene sequencing GC-MS metabolomics analysis investigate effect on microbiome during breast tumorigenesis.Breast tumors stressed non-stressed mice perform...
Abstract Platinum-based drugs remain as the cornerstone of cancer chemotherapy; however, development multidrug resistance presents a therapeutic challenge. This study aims at understanding molecular mechanisms underlying to cisplatin and unraveling surrogate signaling networks that could revert sensitivity apoptosis stimuli. We made use three different sets cell lines, A549 H2030 non-small-cell lung (NSCLC) A2780 ovarian cells their cisplatin-resistant variants. Here we report lines...
Abstract Advances in spatial transcriptomics technologies have enabled the gene expression profiling of tissues while retaining its context. Effective exploitation this data combination requires spatially informed analysis tools to perform three key tasks, clustering, multi-sample integration, and cell type deconvolution. Here, we present GraphST, a novel graph self-supervised contrastive learning method that incorporates location information profiles accomplish all tasks streamlined process...
ABSTRACT Influenza A virus is a respiratory that can cause complications such as acute bronchitis and secondary bacterial pneumonia. Drug therapies vaccines are available against influenza, albeit limited by drug resistance the non-universal vaccine administration. Hence there need for host-targeted influenza to provide an effective alternative therapeutic target. Sec13 was identified novel host interactor of influenza. As member nuclear pore complex coat protein II (COPII) vesicles,...
ABSTRACT Triple-negative breast cancers (TNBC), associated with poor prognosis and high tumour recurrence, are often-treated taxanes in first-line treatment regimens. However, acquired disease resistance can often set in, hampering clinical efficacy. One avenue that could engender therapy is therapy-induced senescence (TIS), as they represent a population of residual highly secretory. Although it known TIS contribute to development via the secretome, underlying molecular mechanisms not fully...
Abstract Triple-negative breast cancers (TNBC), associated with poor prognosis and high tumour recurrence, are often treated taxanes. However, cells may bypass treatment-induced cell death via mitotic slippage, resulting in multinucleated polyploid therapy-induced senescence (TIS). TIS cancer represent a population of residual disease modulate therapy resistance recurrence through secretion the senescence-associated secretory phenotype (SASP). While SASP have been heavily linked...
Abstract Anthracyclines such as doxorubicin remain the first line of treatment for haematological malignancies, and breast cancers. However, potential risk cardiac injury by anthracyclines, which may lead to severe myopathy or heart failure, severely limits their application, remains a challenge ensuring curative chemotherapy. While complex interplay between pathological pathways anthracycline cardiotoxicity is yet be fully understood, oxidative damage, iron overload-mediated formation...