A5070900960- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- MicroRNA in disease regulation
- Sarcoidosis and Beryllium Toxicity Research
- Advanced Chemical Sensor Technologies
- Cancer-related molecular mechanisms research
- Plant-based Medicinal Research
- COVID-19 Clinical Research Studies
- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- Respiratory Support and Mechanisms
- Infectious Diseases and Tuberculosis
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Diagnosis and treatment of tuberculosis
- RNA Interference and Gene Delivery
- Berberine and alkaloids research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Advancements in Transdermal Drug Delivery
- Neonatal Respiratory Health Research
- Respiratory and Cough-Related Research
- Cancer, Hypoxia, and Metabolism
- Congenital Anomalies and Fetal Surgery
- ATP Synthase and ATPases Research
- Cancer Genomics and Diagnostics
- Transgenic Plants and Applications
All India Institute of Medical Sciences
2018-2025
Institute of Genomics and Integrative Biology
2015-2025
All India Institute of Medical Sciences Raipur
2020-2025
All India Institute of Medical Sciences Bhopal
2020-2025
Jamia Millia Islamia
2023
Weatherford College
2023
Ashoka University
2023
Central Leather Research Institute
2020-2021
Centre for Cellular and Molecular Biology
2020-2021
Immungenetics (Germany)
2018
Recently, we showed that generation of tumours in syngeneic mice by cells devoid mitochondrial (mt) DNA (ρ0 cells) is linked to the acquisition host mtDNA. However, mechanism mtDNA movement between remains unresolved. To determine whether transfer involves whole mitochondria, injected B16ρ0 mouse melanoma into C57BL/6Nsu9-DsRed2 express red fluorescent protein their mitochondria. We document acquired mitochondria from animal, leading normalisation respiration. Additionally, knockdown key...
Dynamic variations in mitochondrial shape have been related to function. However, tools automatically classify and enumerate shapes are lacking, as systematic studies exploring the relationship of such stress. Here we show that during increased generation reactive oxygen species (mtROS), mitochondria change their from tubular donut or blob forms, which can be computationally quantified. Imaging cells treated with rotenone antimycin, showed time dose-dependent conversion forms donut-shaped...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Acute respiratory distress syndrome (ARDS) is characterized by an excessive acute inflammatory response in lung parenchyma, which ultimately leads to refractory hypoxemia. One of the earliest abnormalities seen injury elevated levels cytokines, among them, soluble tumor necrosis factor (TNF-α) has a key role, exerts cytotoxicity epithelial and endothelial cells thus exacerbates edema. The bacterial lipopolysaccharide (LPS) was used both vitro (RAW 264.7, THP-1, MLE-12, A549, BEAS-2B) vivo...
There is limited knowledge regarding the consequences of hyperinsulinemia on lung. Given increasing prevalence obesity, insulin resistance, and epidemiological associations with asthma, this a critical lacuna, more so inhaled horizon. Here, we demonstrate that can adversely affect respiratory health. Insulin treatment (1 μg/ml) significantly (P < 0.05) increased proliferation primary human airway smooth muscle (ASM) cells induced collagen release. Additionally, ASM showed significant...
The role of miRNAs in determining human neural stem cell (NSC) fate remains elusive despite their high expression the developing nervous system. In this study, we investigate miR-137, a brain-enriched miRNA, induced pluripotent cells-derived NSCs (hiNSCs). We show that ectopic miR-137 hiNSCs reduces proliferation and accelerates neuronal differentiation migration. TargetScan MicroT-CDS predict myocyte enhancer factor-2A (MEF2A), transcription factor regulates peroxisome...
Abstract Background COVID-19 pneumonia has been associated with severe acute hypoxia, sepsis-like states, thrombosis and chronic sequelae including persisting hypoxia fibrosis. The molecular response pathway such pathologies our recent observations on anti-hypoxic anti-inflammatory effects of whole aqueous extract Adhatoda Vasica (AV) prompted us to explore its relevant preclinical mouse models. Methods In this study, we tested the effect AV, in murine models bleomycin induced pulmonary...
Oxygen-sensing prolyl-hydroxylase (PHD)-2 negatively regulates hypoxia-inducible factor (HIF)1-α and suppresses the hypoxic response. Hypoxia signaling is thought to be proinflammatory but also attenuates cellular injury apoptosis. Although increased response has been noted in asthma, its functional relevance unknown. The objectives of this study were dissect mechanisms role asthma pathophysiology. Experimental studies conducted mice using acute chronic allergic models asthma. allergically...
The present study involves evaluation of antioxidant potential Crocus sativus and its main constituents, safranal (SFN) crocin (CRO), in bronchial epithelial cells, followed antiinflammatory the active constituent safranal, a murine model asthma. To investigate antioxidizing constituents stress was induced these cells by combination different cytokines that resulted an increase nitric oxide production (NO), synthase (iNOS) levels, peroxynitrite ion generation, cytochrome c release. Treatment...
Abstract INPP4A has been shown to be involved in the regulation of cell proliferation and apoptosis multiple types including fibroblasts. Previous reports from our group have demonstrated role inositol polyphosphate 4‐phosphatase Type I A (INPP4A) these functions. Though existing evidences suggest a critical for maintenance lung homeostasis, its chronic diseases is relatively under explored. In current study, we made an attempt understand idiopathic pulmonary fibrosis (IPF). Through...
Abstract Asthma and Chronic Obstructive Pulmonary Disease (COPD) have many common clinical characteristics, thus making reliable differentiation between these two challenging. The goal of this study is to determine the value exhaled breath condensate derived miRNAs discriminate COPD. This cross-sectional included 65 subjects each with asthma (mean/SD age: 39/13 years, Male n/%: 27/42%), COPD 61/9 53/81%) healthy controls 34.4/12 50/77%). Exhaled (EBC) was collected using R-tubes 40 EBC...
The development of sensitive and specific diagnostic tools for hepatitis B virus (HBV) C (HCV) remains crucial effective disease management control. In this study, we utilized CRISPR‐Cas12 CRISPR‐Cas13 systems the detection HBV (DNA virus) HCV (RNA virus), respectively. We designed tested multiple guide RNAs (gRNAs) targeting both viruses, confirming successful cleavage target sequences through gel electrophoresis a fluorescent reporter assay. Using optimized gRNAs, developed lateral flow...
Nucleic acids, both DNA and small RNAs, have emerged as potential therapeutics for the treatment of various lung disorders. However, delivery nucleic acids to lungs is challenging due barrier property imposed by mucus, which further reinforced in disease conditions such chronic obstructive pulmonary asthma. The presence negatively charged mucins imparts electrostatic property, mesh network structure mucus provides steric hindrance system. To overcome this, system either needs be muco-inert...
Quantitation of microRNAs secreted by lung cells can provide valuable information regarding health. Exhaled breath condensate (EBC) offers a non-invasive way to sample the microRNAs, and could be used as diagnostic tools for cancer. EBC samples from twenty treatment-naïve patients with pathologically confirmed cancer healthy subjects were profiled miRNAs expression. Selected further validated, using quantitative-PCR, in an independent set 10 both groups. A total 78 found significantly...