Julia Calzada‐Wack
A5027526549- Cancer, Lipids, and Metabolism
- Adipose Tissue and Metabolism
- Sarcoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Protein Degradation and Inhibitors
- Cancer-related gene regulation
- Genetics and Neurodevelopmental Disorders
- Hedgehog Signaling Pathway Studies
- RNA modifications and cancer
- RNA Research and Splicing
- Cytokine Signaling Pathways and Interactions
- Colorectal Cancer Treatments and Studies
- Genomics and Rare Diseases
- Congenital heart defects research
- Metabolism and Genetic Disorders
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Cell Adhesion Molecules Research
- Cancer-related Molecular Pathways
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Proteomics Techniques and Applications
- Cardiomyopathy and Myosin Studies
- Mitochondrial Function and Pathology
- Cellular transport and secretion
Helmholtz Zentrum München
2015-2025
Center for Environmental Health
2012-2014
Technical University of Munich
2000-2012
Klinikum rechts der Isar
2012
Institute of Groundwater Ecology
2002-2007
Universitat de Barcelona
2003
National Cancer Institute
2003
National Institutes of Health
2003
Ludwig-Maximilians-Universität München
1994
Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children (Dagher, R., and Helman, L. (1999) <i>Oncologist</i> 4, 34–44), whereas medulloblastoma, a highly malignant tumor of cerebellum, accounts for 20% childhood brain tumors (Goodrich, V., Scott, M. P. (1998) <i>Neuron</i> 21, 1243–1257). Both are associated with deficiency suppressor<i>Patched</i> (<i>PTCH</i>) Gorlin syndrome (Gorlin, R. J. (1987) <i>Medicine</i> (<i>Baltimore</i>) 66, 98–113), they present corresponding...
The hormone calcitonin (CT) is primarily known for its pharmacologic action as an inhibitor of bone resorption, yet CT-deficient mice display increased formation. These findings raised the question about underlying cellular and molecular mechanism CT action. Here we show that either ubiquitous or osteoclast-specific inactivation murine receptor (CTR) causes negatively regulates osteoclast expression Spns2 gene, which encodes a transporter signalling lipid sphingosine 1-phosphate (S1P)....
Covalent chemical modifications of cellular RNAs directly impact all biological processes. However, our mechanistic understanding the enzymes catalyzing these modifications, their substrates and functions, remains vague. Amongst RNA N 6 -methyladenosine (m A) is widespread found in messenger (mRNA), ribosomal (rRNA), noncoding RNAs. Here, we undertook a systematic screen to uncover new methyltransferases. We demonstrate that methyltransferase-like 5 (METTL5) protein catalyzes m A 18S rRNA at...
RNA methyltransferase METTL6 is implicated in tumor cell growth and mouse energy consumption.
Clinical presentation of congenital heart disease is heterogeneous, making identification the disease-causing genes and their genetic pathways mechanisms action challenging. By using in vivo electrocardiography, transthoracic echocardiography microcomputed tomography imaging to screen 3,894 single-gene-null mouse lines for structural functional cardiac abnormalities, here we identify 705 with arrhythmia, myocardial hypertrophy and/or ventricular dilation. Among these genes, 486 have not been...
Abstract CD14, a glycolipid‐anchored membrane glycoprotein, acts as high affinity lipopolysaccharide receptor on leukocytes. We previously reported that the Mono‐Mac‐6 cell line releases two different soluble forms of CD14 (sCD14) (Labeta et al. Eur. J. Immunol. 1993. 23 : 2144). Here we show sCD 14, which now refer to 14α (low M r ), and 14β (high are also synthesized released by normal human monocytes present in plasma. Their mechanism release was examined using line, chinese hamster ovary...
ADAR2, an RNA editing enzyme that converts specific adenosines to inosines in certain pre-mRNAs, often leading amino acid substitutions the encoded proteins, is mainly expressed brain. Of all ADAR2-mediated edits, a single one pre-mRNA of AMPA receptor subunit GluA2 essential for survival. Hence, early postnatal death mice lacking ADAR2 averted when critical edit engineered into both encoding Gria2 alleles. Adar2−/−/Gria2R/R display normal appearance and life span, but general phenotypic...
Accidental nuclear scenarios lead to environmental contamination of unknown level. Immediate radiation-induced biological responses that trigger processes leading adverse health effects decades later are not well understood. A comprehensive proteomic analysis provides a promising means identify and quantify the initial damage after radiation exposure. Early changes in cardiac tissue C57BL/6 mice exposed total body irradiation were studied, using dose relevant both intentional accidental...
Formalin-fixed paraffin-embedded (FFPE) tissue has recently gained interest as an alternative to fresh/frozen for retrospective protein biomarker discovery. However, during the fixation process, proteins undergo degradation and cross-linking, making conventional analysis technologies problematic. In this study, we have compared several extraction separation methods of in FFPE tissues. Incubation sections at high temperature with a novel buffer (20 mM Tris-HCl, pH 8.8, 2% SDS, 1%...
High attrition rates of novel anti-cancer drugs highlight the need for improved models to predict toxicity. Although polo-like kinase 1 (Plk1) inhibitors are attractive candidates drug development, role Plk1 in primary cells remains widely unexplored. Therefore, we evaluated utility an RNA interference-based model assess responses inducible knockdown (iKD) adult mice. Here show that silencing can be achieved several organs, although adverse events rare. We compared Plk1-iKD mice with those...
Abstract Current concepts regarding the biology of aging are primarily based on studies aimed at identifying factors regulating lifespan. However, lifespan as a sole proxy measure for can be limited value because it may restricted by specific pathologies. Here, we employ large-scale phenotyping to analyze hundreds markers in male C57BL/6J mice. For each phenotype, establish lifetime profiles determine when age-dependent change is first detectable relative young adult baseline. We examine key...
Rare diseases (RDs) are a challenge for medicine due to their heterogeneous clinical manifestations and low prevalence. There is lack of specific treatments only few hundred the approximately 7,000 RDs have an approved regime. Rapid technological development in genome sequencing enables mass identification potential candidates that mutated form could trigger but often not confirmed be causal. Knockout (KO) mouse models essential understand causality genes by allowing highly standardized...
MIM/MTSS1 is a tissue-specific regulator of plasma membrane dynamics, whose altered expression levels have been linked to cancer metastasis. MIM deforms phosphoinositide-rich membranes through its I-BAR domain and interacts with actin monomers WH2 domain. Recent work proposed that also potentiates Sonic hedgehog (Shh)-induced gene expression. Here, we generated mutant mice found full-length protein dispensable for embryonic development. However, MIM-deficient displayed severe urinary...
Iron Regulatory Protein 2 (Irp2, Ireb2) is a central regulator of cellular iron homeostasis in vertebrates. Two global knockout mouse models have been generated to explore the role Irp2 regulating metabolism. While both show that loss results microcytic anemia and altered body distribution, discrepant drawn into question brain One model shows aged deficient mice develop adult-onset progressive neurodegeneration associated with axonal degeneration Purkinje cells nervous system. These...
Ewing sarcoma, an osteolytic malignancy that mainly affects children and young adults, is characterized by early metastasis to lung bone. In this study, we identified the pro-metastatic gene DKK2 as a highly overexpressed in sarcoma compared with corresponding normal tissues. Using RNA interference, showed was critical for malignant cell outgrowth vitro orthotopic xenograft mouse model vivo. Analysis of invasion potential both settings revealed strong correlation expression invasiveness may...
Cln3Δex7/8 mice harbor the most common genetic defect causing juvenile neuronal ceroid lipofuscinosis (JNCL), an autosomal recessive disease involving seizures, visual, motor and cognitive decline, premature death. Here, to more thoroughly investigate manifestations of JNCL mutation, we performed a broad phenotyping study mice. Homozygous mice, congenic on C57BL/6N background, displayed subtle deficits in sensory tasks at 10–14 weeks age. also electroretinographic changes reflecting cone...
// Tim Hensel 1,2* , Chiara Giorgi 3,* Oxana Schmidt 1,2 Julia Calzada-Wack 4 Frauke Neff Thorsten Buch 5,6 Felix K. Niggli 3 Beat W. Schäfer Stefan Burdach and Günther H.S. Richter 1 Laboratory for Functional Genomics Transplantation Biology, Children’s Cancer Research Centre Department of Pediatrics, Klinikum rechts der Isar, Technische Universität München, Munich, Germany 2 Comprehensive Center Munich (CCCM), Oncology Center, University Hospital, Zurich,...