A5041592278- Advanced Proteomics Techniques and Applications
- Protein Degradation and Inhibitors
- Mass Spectrometry Techniques and Applications
- Pancreatic and Hepatic Oncology Research
- Gut microbiota and health
- Free Radicals and Antioxidants
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Protein Tyrosine Phosphatases
- Toxic Organic Pollutants Impact
- Advanced biosensing and bioanalysis techniques
- Cancer Immunotherapy and Biomarkers
- Phagocytosis and Immune Regulation
- Advanced Biosensing Techniques and Applications
- Clostridium difficile and Clostridium perfringens research
- Cardiomyopathy and Myosin Studies
- Probiotics and Fermented Foods
- Cancer Research and Treatments
- Biodiesel Production and Applications
- Epigenetics and DNA Methylation
- Metabolomics and Mass Spectrometry Studies
- Ubiquitin and proteasome pathways
- Molecular Biology Techniques and Applications
Technical University of Munich
2019-2024
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2020
Although most cancer drugs modulate the activities of cellular pathways by changing posttranslational modifications (PTMs), little is known regarding extent and time- dose-response characteristics drug-regulated PTMs. In this work, we introduce a proteomic assay called decryptM that quantifies drug-PTM modulation for thousands PTMs in cells to shed light on target engagement drug mechanism action. Examples range from detecting DNA damage chemotherapeutics, identifying drug-specific PTM...
Infections by multidrug-resistant Enterobacteriaceae (MRE) are life-threatening to patients. The intestinal microbiome protects against MRE colonization, but antibiotics cause collateral damage commensals and open the way colonization subsequent infection. Despite significance of this problem, specific mechanisms that restrict remain largely unknown. Here, performing a multi-omic prospective study hospitalized patients combined with mice experiments, we find Lactobacillus is key, though not...
The microbiome has a strong impact on human health and disease is, therefore, increasingly studied in clinical context. Metaproteomics is also attracting considerable attention, such data can be efficiently generated today owing to improvements mass spectrometry-based proteomics. As we will discuss this study, there are still major challenges notably analysis that need overcome. Here, analyzed 212 fecal samples from 56 hospitalized acute leukemia patients with multidrug-resistant...
LIMKs are important regulators of actin and microtubule dynamics, they play essential roles in many cellular processes. Deregulation has been linked to the development diverse diseases, including cancers cognitive disabilities, but well-characterized inhibitors known as chemical probes still lacking. Here, we report characterization three highly selective LIMK1/2 covering all canonical binding modes (type I/II/III) structure-based design type II/III inhibitors. Characterization these...
Abstract Kinase inhibitors (KIs) are important cancer drugs but often feature polypharmacology that is molecularly not understood. This disconnect particularly apparent in entities such as sarcomas for which the oncogenic drivers clear. To investigate more systematically how cellular proteotypes of sarcoma cells shape their response to targeted drugs, we profiled proteomes and phosphoproteomes 17 cell lines screened same against 150 drugs. The resulting 2550 phenotypic profiles revealed...
3068 Background: Precision oncology approaches employing genomics-guided targeted therapies for individual patients have provided significant survival benefits in several cancer types. However, varying response rates solid malignancies, many without actionable genomic lesions, and increasing evidence that non-genomic mechanisms may play an important role tumors indicate genomics alone is often insufficient to inform guide the clinical care of patients. Since most anti-cancer drugs inhibit...
Aromatic carotenoid cleavage products as alternative to conventional alkyl-phenolic antioxidants for jet fuel and beyond.
<p class="first" dir="auto" id="d14302121e292">While DNA and RNA sequencing are increasingly applied in precision oncology, the therapeutically important (phospho)proteome is rarely included. We implemented a clinical proteomics workflow into two existing molecular tumour board programs. Here, we present semi-automated analysis of (phospho)proteomic data allowing therapeutic suggestion within turnaround time weeks. Currently, cohort consists >500 individual patient samples from various...