Violeta Muñoz‐Fuentes
A5004693476- Wildlife Ecology and Conservation
- Genetic diversity and population structure
- Identification and Quantification in Food
- Genetic and phenotypic traits in livestock
- Yersinia bacterium, plague, ectoparasites research
- Bioinformatics and Genomic Networks
- Biomedical Text Mining and Ontologies
- Single-cell and spatial transcriptomics
- Livestock and Poultry Management
- Molecular Biology Techniques and Applications
- Human-Animal Interaction Studies
- Animal Ecology and Behavior Studies
- Bat Biology and Ecology Studies
- Gene expression and cancer classification
- Genomics and Rare Diseases
- High Altitude and Hypoxia
- Physiological and biochemical adaptations
- Avian ecology and behavior
- Metabolomics and Mass Spectrometry Studies
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Genetic Mapping and Diversity in Plants and Animals
- Statistical Methods in Clinical Trials
- bioluminescence and chemiluminescence research
- Fish Biology and Ecology Studies
World Conservation Monitoring Centre
2024-2025
European Bioinformatics Institute
2017-2024
Wellcome Trust
2017-2023
Senckenberg Research Institute and Natural History Museum Frankfurt/M
2014-2020
Estación Biológica de Doñana
2006-2017
Uppsala University
2006-2015
Consejo Superior de Investigaciones Científicas
2005-2009
Abstract The International Mouse Phenotyping Consortium (IMPC; https://www.mousephenotype.org/) web portal makes available curated, integrated and analysed knockout mouse phenotyping data generated by the IMPC project consisting of 85M points over 95,000 statistically significant phenotype hits mapped to human diseases. delivers a substantial reference dataset that supports enrichment various domain-specific projects databases, as well wider research clinical community, where...
Species persistence can be influenced by the amount, type, and distribution of diversity across genome, suggesting a potential relationship between historical demography resilience. In this study, we surveyed genetic variation single genomes 240 mammals that compose Zoonomia alignment to evaluate how effective population size (
A fundamental question in evolutionary genetics concerns the extent to which adaptive phenotypic convergence is attributable convergent or parallel changes at molecular sequence level. Here we report a comparative analysis of hemoglobin (Hb) function eight phylogenetically replicated pairs high- and low-altitude waterfowl taxa test for oxygenation properties Hb, assess biochemical phenotype repeated amino acid replacements. Functional experiments on native Hb variants protein engineering...
Abstract Noninvasively collected samples are a common source of DNA in wildlife genetic studies. Currently, single nucleotide polymorphism (SNP) genotyping using microfluidic arrays is emerging as an easy-to-use and cost-effective methodology. Here we assessed the performance SNP noninvasive from grey wolves, European wildcats brown bears, compared results with traditional microsatellite genotyping. We successfully SNP-genotyped 87%, 80% 97% wolf, cat bear samples, respectively. Genotype...
Abstract The International Mouse Phenotyping Consortium (IMPC) is building a catalogue of mammalian gene function by producing and phenotyping knockout mouse line for every protein-coding gene. To date, the IMPC has generated characterised 5186 mutant lines. One-third lines have been found to be non-viable over 300 new models human disease identified thus far. While current bioinformatics efforts are focused on translating results better understand processes, data also aids understanding...
The identification of causal variants in sequencing studies remains a considerable challenge that can be partially addressed by new gene-specific knowledge. Here, we integrate measures how essential gene is to supporting life, as inferred from viability and phenotyping screens performed on knockout mice the International Mouse Phenotyping Consortium essentiality carried out human cell lines. We propose cross-species classification across Full Spectrum Intolerance Loss-of-function (FUSIL)...
Clinical presentation of congenital heart disease is heterogeneous, making identification the disease-causing genes and their genetic pathways mechanisms action challenging. By using in vivo electrocardiography, transthoracic echocardiography microcomputed tomography imaging to screen 3,894 single-gene-null mouse lines for structural functional cardiac abnormalities, here we identify 705 with arrhythmia, myocardial hypertrophy and/or ventricular dilation. Among these genes, 486 have not been...
The ruddy duck, Oxyura jamaicensis, was introduced to Great Britain in the mid-20th century and has recently spread other Western European countries. In Spain, ducks hybridize with globally endangered white-headed leucocephala. We assessed effects of hybridization on Spanish ducks, which constitute 25% global population this species, using a panel eight nuclear intron markers, 10 microsatellite loci, mtDNA control region sequences. These data allowed parental individuals, F(1) hybrids,...
Abstract Aim Limited population structure is predicted for vagile, generalist species, such as the grey wolf ( Canis lupus L.). Our aims were to study how genetic variability of wolves was distributed in an area comprising different habitats that lay within potential dispersal range individual and make inferences about impact ecology on structure. Location British Columbia, Canada – which characterized by a continuum biogeoclimatic zones across are adjacent areas both Alaska, United States....
Abstract Aim Pleistocene environmental fluctuations had well‐characterized impacts on the patterns of within‐species divergences and diversity in temperate habitats. Here we examine impact widely distributed forest vertebrates a tropical system where distribution habitat was affected by those fluctuations. Location Sundaland, Southeast Asia. Methods We conducted comparative phylogeographical analysis 28 non‐migratory, forest‐dependent vertebrates, for which constructed rooted, intraspecifc...
The European wildcat population in Scotland is considered critically endangered as a result of hybridization with introduced domestic cats,1,2 though the time frame over which this gene flow has taken place unknown. Here, using genome data from modern, museum, and ancient samples, we reconstructed trajectory dated decline local viable to severely hybridized. We demonstrate that although cats have been present Britain for 2,000 years,3 onset was only within last 70 years. Our analyses reveal...
Meiotic recombination is a fundamental process needed for the correct segregation of chromosomes during meiosis in sexually reproducing organisms. In humans, 80% crossovers are estimated to occur at specific areas genome called hotspots. Recently, protein PRDM9 was identified as major player determining location genome-wide meiotic hotspots humans and mice. The origin this seems be ancient evolutionary time, reflected by its fairly conserved structure lineages that diverged over 700 million...
Abstract Following protection measures implemented since the 1970s, large carnivores are currently increasing in number and returning to areas from which they were absent for decades or even centuries. Monitoring programmes these species rely extensively on non‐invasive sampling genotyping. However, attempts connect results of such studies at larger spatial temporal scales often suffer incompatibility genetic markers by researchers different laboratories. This is particularly critical...
Recombination rates vary in intensity and location at the species, individual, sex chromosome levels. Despite fundamental biological importance of this process, selective forces that operate to shape recombination rate patterns are unclear. Domestication offers a unique opportunity study interplay between selection. In domesticates, intense selection for particular traits is imposed on small populations over many generations, resulting organisms differ, sometimes dramatically, morphology...
Abstract Background Understanding the processes that lead to hybridization of wolves and dogs is scientific management importance, particularly over large geographical scales, as can disperse great distances. However, a method efficiently detect hybrids in routine wolf monitoring lacking. Microsatellites offer only limited resolution due low number markers showing distinctive allele frequencies between dogs. Moreover, calibration across laboratories time-consuming costly. In this study, we...
Identification of predators from saliva traces on game species and/or livestock kills is gaining increasing importance in wildlife management, particularly areas where direct wildlife–human conflicts regularly occur. When the noninvasive sampling hairs and scats difficult, as with rare elusive predators, samples constitute a potentially useful source DNA. To test feasibility this approach obtaining an accurate genotype predator, we applied experimental approach. Captive wolves (Canis lupus)...
Abstract Hybridization between wild species and their domestic congeners is considered a major threat for wildlife conservation. Genetic integrity of the European wildcat, instance, concern as they are outnumbered by cats several orders magnitude throughout its range. We genotyped 1,071 individual wildcat samples obtained from hair traps roadkills collected across highly fragmented forests western Central Europe, in Germany Luxembourg, to assess cat introgression wildcats human‐dominated...
Phenotypic data are critical for understanding biological mechanisms and consequences of genomic variation, pivotal clinical use cases such as disease diagnostics treatment development. For over a century, vast quantities phenotype have been collected in many different contexts covering variety organisms. The emerging field phenomics focuses on integrating interpreting these to inform hypotheses. A major impediment is the wide range distinct disconnected approaches recording observable...
Domestic cats were derived from the Near Eastern wildcat (Felis lybica), after which they dispersed with people into Europe. As did so, it is possible that interbred indigenous population of European wildcats silvestris). Gene flow between incoming domestic animals and closely related wild species has been previously demonstrated in other taxa, including pigs, sheep, goats, bees, chickens, cattle. In case cats, a lack nuclear, genome-wide data, particularly wildcats, made difficult to either...
Local adaptation frequently occurs across populations as a result of migration-selection balance between divergent selective pressures and gene flow associated with life in heterogeneous landscapes. Studying the effects selection on process can be achieved systems that have recently colonized extreme environments. This study utilizes an endemic South American duck species, speckled teal (Anas flavirostris), which has both high- low-altitude populations. High-altitude (A. f. oxyptera) are...