A5080639659- interferon and immune responses
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Cytokine Signaling Pathways and Interactions
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Influenza Virus Research Studies
- Respiratory viral infections research
- Cancer-related Molecular Pathways
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Tryptophan and brain disorders
- Glycosylation and Glycoproteins Research
- Toxoplasma gondii Research Studies
- RNA modifications and cancer
- Inflammasome and immune disorders
- COVID-19 Clinical Research Studies
Universidade Estadual de Santa Cruz
2025
Helmholtz Centre for Infection Research
2015-2024
Otto-von-Guericke University Magdeburg
2015-2024
University Hospital Magdeburg
2023
Center for Behavioral Brain Sciences
2021-2023
Umeå University
2018
Public Health Agency of Sweden
2018
Universität der Bundeswehr München
2018
Institut für Mikrobiologie der Bundeswehr
2018
Charles River Laboratories (Germany)
2018
Article22 May 2012Open Access Multi-layered stochasticity and paracrine signal propagation shape the type-I interferon response Ulfert Rand Department of Gene Regulation Differentiation, Helmholtz Centre for Infection Research, Braunschweig, Germany Division Theoretical Systems Biology, German Cancer Research Center (DKFZ) BioQuant Center, Heidelberg, Search more papers by this author Melanie Rinas Johannes Schwerk Gesa Nöhren Linnes Andrea Kröger Michael Flossdorf Kristóf Kály-Kullai...
Metastases are the major cause of death from cancer. Thus, understanding regulation metastatic processes is utmost importance. Here we show that mice with impaired type I IFN signaling (Ifnar1(-/-)) develop more lung metastases in 4T1 mammary and LLC carcinoma model, compared to control mice. In Ifnar1(-/-) mice, higher metastasis load accompanied by massive neutrophil accumulation lungs. Elevated G-CSF levels serum enhanced CXCR2 expression on neutrophils most likely responsible for this...
Neurotropic flaviviruses such as tick-borne encephalitis virus (TBEV), Japanese (JEV), West Nile (WNV), and Zika (ZIKV) are causative agents of severe brain-related diseases including meningitis, encephalitis, microcephaly. We have previously shown that local type I interferon response within the central nervous system (CNS) is involved in protection mice against flavivirus infection. However, cells responsible for mounting this protective not defined.Primary astrocytes were isolated from...
Abstract COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2, new recently emerged sarbecovirus. This virus uses the human ACE2 enzyme as receptor for cell entry, recognizing it with binding domain (RBD) of S1 subunit viral spike protein. We present use phage display to select anti-SARS-CoV-2 antibodies from naïve antibody gene libraries HAL9/10 and subsequent identification 309 unique fully against S1. 17 are RBD, showing inhibition cells expressing scFv-Fc neutralize active...
Recent evidence suggests that regulatory pathways might control sustained high levels of FOXP3 in CD4(+)CD25(hi) T (T(reg)) cells. Based on transcriptional profiling ex vivo activated T(reg) and helper CD4(+)CD25(-) (T(h)) cells we have identified GARP (glycoprotein-A repetitions predominant), LGALS3 (lectin, galactoside-binding, soluble, 3) LGMN (legumain) as novel genes implicated human cell function, which are induced upon T-cell receptor stimulation. Retroviral overexpression...
A subgroup of genes induced by IFN-γ requires both STAT1 and IRF1 for transcriptional activation. Using WT, stat1 −/− , or irf1 cells, we analyzed the changes in gbp2 promoter chromatin. associated with independently played an essential role ordered recruitment coactivator/histone acetyl transferase CREB-binding protein (CBP) histone deacetylase HDAC1. Hyperacetylation 4 also required STAT1. Phosphorylation at S727 transactivating domain increased activity In cells expressing a...
Viperin is an antiviral protein whose expression highly upregulated during viral infections via IFN-dependent and/or IFN-independent pathways. We examined the molecular alterations induced by transcriptional activator IFN regulatory factor (IRF)-1 and found viperin to be among group of IRF-1 regulated genes. From these data, it was not possible distinguish genes that are primary targets those IRF-1-induced proteins, like IFN-beta. In this study, we show directly binds murine promoter two...
ABSTRACT Vector-borne flaviviruses, such as tick-borne encephalitis virus (TBEV), West Nile virus, and dengue cause millions of infections in humans. TBEV causes a broad range pathological symptoms, ranging from meningitis to severe or even hemorrhagic fever, with high mortality. Despite the availability an effective vaccine, incidence is increasing. Not much known about role innate immune system control infections. Here, we show that type I interferon (IFN) essential for protection against...
Although type I interferons (IFNs)—key effectors of antiviral innate immunity are known to be induced via different pattern recognition receptors (PRRs), the cellular source and relative contribution PRRs in host protection against viral infection is often unclear. IPS-1 a downstream adaptor for retinoid-inducible gene (RIG-I)-like receptor signaling. In this study, we investigate immune response brain regions during with tick-borne encephalitis virus (TBEV), flavivirus that causes variety...
The importance of neutrophils in tumor immune surveillance, invasive growth and angiogenesis becomes increasingly clear. Many neutrophil activities are controlled by endogenous IFN-β. Here, we provide evidence that IFN-β is regulating the apoptosis pro-angiogenic infiltrating influencing both, extrinsic as well intrinsic pathways. Accordingly, life span associated (TANs) remarkably prolonged bearing Ifnb1(-/-) mice compared to wild type controls. Lower expression Fas, reactive oxygen...
Influenza A virus (IAV) causes respiratory tract disease and is responsible for seasonal reoccurring epidemics affecting all age groups. Next to typical symptoms, such as fever fatigue, IAV infection has been associated with behavioral alterations presumably contributing the development of major depression.
Recent emergence of SARS-CoV-1 variants demonstrates the potential this virus for targeted evolution, despite its overall genomic stability. Here we show dynamics and mechanisms behind rapid adaptation SARS-CoV-2 to growth in Vero E6 cells. The selective advantage cells is due increased cleavage efficiency by cathepsins at mutated S1/S2 site. site also constitutes a heparan sulfate (HS) binding motif that influenced cells, but HS antagonist did not inhibit these entry E6-adapted into human...
Balanced induction of proinflammatory and type I IFN responses upon activation Toll-like receptors (TLRs) determines the outcome microbial infections pathogenesis autoimmune other inflammatory diseases. Mast cells, key components innate immune system, are known for their debilitating role in allergy autoimmunity. However, antimicrobial host defenses is being acknowledged increasingly. How mast cells interact with microbes nature triggered thereby not well characterized. Here we show that...
ABSTRACT Influenza A virus (IAV) periodically causes substantial morbidity and mortality in the human population. In lower lung, primary targets for IAV replication are type II alveolar epithelial cells (AECII), which increasingly recognized their immunological potential. So far, little is known about reaction to contribution respiratory antiviral immunity vivo . Therefore, we characterized AECII response during early infection by analyzing transcriptional regulation sorted from lungs of...
The importance of endogenous Type I IFNs in cancer immune surveillance is well established by now. Their role polarization tumor-associated neutrophilic granulocytes into anti-tumor effector cells has been recently demonstrated. Yet, the cellular source as mode induction not clearly defined. Here, we demonstrate that IFN-β induced growing murine tumors. Induction mainly mediated via STING-dependent signaling pathways, suggesting tumor derived DNA trigger. Transcription factors IRF3 and IRF5...
Abstract In bacterial and sterile inflammation of the liver, hepatocyte apoptosis is, in contrast to necroptosis, a common feature. The molecular mechanisms preventing necroptosis potential consequences are largely unknown. Apoptosis critically regulated by ubiquitination signaling molecules but especially regulatory function deubiquitinating enzymes (DUBs) is imperfectly defined. Here, we addressed role DUB OTU domain aldehyde binding-1 (OTUB1) cell death upon both infection with...
Moniliophthora perniciosa is one of the main pathogens affecting cocoa crop. The control this phytopathogen generally carried out by means resistant genotypes accompanied phytosanitary pruning. identification plant genes related to defense mechanism important unravel molecular basis plant-pathogen interaction. One potential gene studied binding protein (BiP), a chaperone located in lumen endoplasmic reticulum that binds newly synthesized proteins as they are translocated into ER and...
Moniliophthora perniciosa is one of the main pathogens affecting cocoa, and controlling it generally involves planting resistant genotypes followed by phytosanitary pruning. The identification plant genes related to defense mechanisms crucial unravel molecular basis plant-pathogen interactions. Among candidate genes, BiP stands out as a chaperone located in endoplasmic reticulum that facilitates protein folding induced under stress conditions, such pathogen attacks. In this study, SoyBiPD...
Abstract The CD11c + cell population in the non‐parenchymal of mouse liver contains dendritic cells (DC), NK cells, B and T cells. In hepatic DC from immunocompetent or immunodeficient [recombinase‐activating gene‐1 (RAG1) –/– ] C57BL/6 mice (rigorously depleted cells), we identified a B220 int subset ‘plasmacytoid’ DC, – subset. latter could be subdivided into major, immature (CD40 lo CD80 CD86 MHC class II ) subset, minor, mature hi Stimulated but not produced type I interferon. NKT...
Abstract Pleiotropic, immunomodulatory effects of type I IFN on T cell responses are emerging. We used vaccine-induced, antiviral CD8+ in IFN-β (IFN-β−/−)- or receptor (IFNAR−/−)-deficient mice to study immunomodulating that not complicated by the interference a concomitant virus infection. Compared with normal B6 mice, IFNAR−/− IFN-β−/− have numbers CD4+ and cells, CD25+FoxP3+ regulatory (TR) cells liver spleen. Twice as many specific for different class I-restricted epitopes develop than...
Only humans and chimpanzees are susceptible to chronic infection by hepatitis C virus (HCV). The restricted species tropism of HCV is determined distinct host factor requirements at different steps the viral life cycle. In addition, effective innate immune targeting precludes efficient propagation in nonhuman cells. Species-specificity usage for cell entry release has been explored. However, reason inefficient RNA replication efficiency mouse liver cells remains elusive. To address this, we...
The tick-borne encephalitis virus (TBEV) is a flavivirus transmitted to humans, usually via tick bites. causes (TBE) in and symptoms range from mild flu-like severe long-lasting sequelae, including permanent brain damage. It has been suggested that within the population of viruses mammalian host, quasispecies with neurotropic properties might become dominant host resulting neurological symptoms. We previously demonstrated existence TBEV variants variable poly(A) tracts single blood-fed tick....