A5049849598- Hepatitis C virus research
- Bioinformatics and Genomic Networks
- Hepatitis B Virus Studies
- SARS-CoV-2 and COVID-19 Research
- Liver Disease Diagnosis and Treatment
- HIV Research and Treatment
- interferon and immune responses
- Gene expression and cancer classification
- Gene Regulatory Network Analysis
- SARS-CoV-2 detection and testing
- Neuroblastoma Research and Treatments
- Biosensors and Analytical Detection
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Advanced biosensing and bioanalysis techniques
- ECG Monitoring and Analysis
- Artificial Intelligence in Healthcare
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Microbial Metabolic Engineering and Bioproduction
Universitätsmedizin Greifswald
2016-2025
German Centre for Cardiovascular Research
2019-2025
Universität Greifswald
2017-2024
Institute of Bioinformatics and Systems Biology
2024
Heidelberg University
2009-2023
TU Dresden
2011-2017
University Hospital Carl Gustav Carus
2012-2016
Zimmer Biomet (Germany)
2012-2015
Carl Gustav Carus-Institut
2014
National Institutes of Health
2011
Standard cancer therapy targets tumor cells without considering possible damage on the microenvironment that could impair response. In rectal patients we find inflammatory cancer-associated fibroblasts (iCAFs) are associated with poor chemoradiotherapy Employing a murine model or patient-derived organoids and primary stroma cells, show that, upon irradiation, interleukin-1α (IL-1α) not only polarizes toward phenotype but also triggers oxidative DNA damage, thereby predisposing iCAFs to...
In coronavirus disease 2019 (COVID-19), hypertension and cardiovascular diseases are major risk factors for critical progression. However, the underlying causes effects of main anti-hypertensive therapies—angiotensin-converting enzyme inhibitors (ACEIs) angiotensin receptor blockers (ARBs)—remain unclear. Combining clinical data (n = 144) single-cell sequencing airway samples 48) with in vitro experiments, we observed a distinct inflammatory predisposition immune cells patients that...
... The Study of Health in Pomerania (SHIP) comprises the two independent cohorts SHIP-START (recruited between 1997 and 2001) SHIP-TREND 2008 to 2012), which were established examine health disease status general adult population Northeast Germany given that this region had lowest life expectancy 1990s.1 initial cohort was renamed avoid confusion with designation SHIP, as whole project different cohorts. Both serve purpose understanding concepts their greatest possible complexity, rather...
Abstract Background Sepsis and inflammation can cause intensive care unit‐acquired weakness (ICUAW). Increased interleukin‐6 (IL‐6) plasma levels are a risk factor for ICUAW. IL‐6 signalling involves the glycoprotein 130 (gp130) receptor JAK/STAT‐pathway, but its role in sepsis‐induced muscle wasting is uncertain. In clinical observational study, we found that target gene, SOCS3 , was increased skeletal of ICUAW patients indicative JAK/STAT‐pathway activation. We tested hypothesis...
Since autumn 2020, rapid antigen tests (RATs) have been implemented in several countries as an important pillar of the national testing strategy to rapidly screen for infections on site during SARS-CoV-2 pandemic. The current surge infection rates around globe is driven by variant concern (VoC) omicron (B.1.1.529). Here, we evaluated performance nine RATs a single-centre laboratory study. We examined total 115 PCR-negative and 166 PCR-positive respiratory swab samples (101 omicron, 65 delta...
Hepatitis B viruses (HBVs), which are enveloped with reverse-transcribed DNA genomes, constitute the family Hepadnaviridae. An outstanding feature of HBVs is their streamlined genome organization extensive gene overlap. Remarkably, ∼1,100 bp open reading frame (ORF) encoding envelope proteins fully nested within ORF viral replicase P. Here, we report discovery a diversified fish viruses, designated nackednaviruses, lack protein gene, but otherwise exhibit key characteristics including...
Changes in the airway microbiome may be important pathophysiology of chronic lung disease patients with cystic fibrosis. However, little is known about early fibrosis and relationship between microbiomes from different niches upper lower airways. Therefore, this cross-sectional study, we examined (nose throat) (sputum) airways children using next generation sequencing. Our results demonstrate a significant difference both α β-diversity nose two other sampling sites. The nasal was...
OBJECTIVES: Autoimmune pancreatitis (AIP) is thought to be an immune-mediated inflammatory process, directed against the epithelial components of pancreas. The objective was identify novel markers disease and unravel pathogenesis AIP. METHODS: To explore key targets we analyzed expression proteins at RNA protein level using genomics proteomics, immunohistochemistry, western blot, immunoassay. An animal model AIP with LP-BM5 murine leukemia virus-infected mice studied in parallel. microarrays...
Abstract Persistent infection with hepatitis C virus (HCV) can lead to chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. All current therapies of include interferon-alpha (IFN-α). Moreover, IFN-gamma (IFN-γ), the only type II IFN, strongly inhibits HCV replication in vitro is primary mediator HCV-specific antiviral T-cell responses. However, for both cytokines precise set effector protein(s) responsible inhibition not known. The aim this study was identification IFN-α IFN-γ...
To evaluate the impact of a predefined gene expression-based classifier for clinical risk estimation and cytotoxic treatment decision making in neuroblastoma patients.Gene expression profiles 440 internationally collected specimens were investigated by microarray analysis, 125 which examined prospectively. Patients classified as either favorable or unfavorable 144-gene prediction analysis microarrays (PAM) established previously on separate set 77 patients. PAM classification results...
Hepatitis C virus (HCV) has infected around 160 million individuals. Current therapies have limited efficacy and are fraught with side effects. To identify cellular HCV dependency factors, possible therapeutic targets, we manipulated signaling cascades pathway-specific inhibitors. Using this approach identified the MAPK/ERK regulated, cytosolic, calcium-dependent, group IVA phospholipase A2 (PLA2G4A) as a novel factor. Inhibition of PLA2G4A activity reduced core protein abundance at lipid...
RIG-I is a major innate immune sensor for viral infection, triggering an interferon (IFN)-mediated antiviral response upon cytosolic detection of RNA. Double-strandedness and 5'-terminal triphosphates were identified as motifs required to elicit optimal immunological signaling. However, very little known about the dynamics pathway, which crucial ability cell react diverse classes RNA while maintaining self-tolerance. In present study, we addressed molecular mechanism signal its translation...
Hepatitis C virus (HCV) p7 is a membrane-associated ion channel protein crucial for production. To analyze how contributes to this process, we dissected HCV morphogenesis into sub-steps including recruitment of core lipid droplets (LD), capsid assembly, unloading from LDs and subsequent membrane envelopment capsids. Interestingly, observed accumulation slowly sedimenting capsid-like structures lacking the viral envelope in cells transfected with mutant genomes which possess defect virion...
ABSTRACT Hepatitis C virus (HCV) predominantly infects human hepatocytes, although extrahepatic reservoirs are being discussed. Infection of cells is initiated via cell-free and direct cell-to-cell transmission routes. Cell type-specific determinants HCV entry RNA replication have been reported. Moreover, several host factors required for synthesis secretion lipoproteins from liver cells, in part expressed tissue-specific fashion, implicated assembly. However, the minimal cell requirements...
Similar to other positive-strand RNA viruses, hepatitis C virus (HCV) causes massive rearrangements of intracellular membranes, resulting in a membranous web (MW) composed predominantly double-membrane vesicles (DMVs), the presumed sites replication. DMVs are enriched for cholesterol, but mechanistic details on source and recruitment cholesterol viral replication organelle only partially known. Here we focused selected lipid transfer proteins implicated direct at various endoplasmic...
Electrocardiography is the most common tool to diagnose cardiovascular diseases. Annotation, segmentation and rhythm classification of ECGs are challenging tasks, especially in presence atrial fibrillation other arrhythmias. Our aim increase accuracy heart estimation by use extreme gradient boosting trees development a deep convolutional neural network for ECG segmentation.We trained with waveforms from PhysioNet databases annotate QRS complexes, P waves, T noise interbeat segments that...
Abstract Successful containment strategies for the SARS-CoV-2 pandemic will depend on reliable diagnostic assays. Point-of-care antigen tests (POCT) may provide an alternative to time-consuming PCR rapidly screen acute infections site. Here, we evaluated two tests: STANDARD™ F COVID-19 Ag FIA (FIA) and Rapid Antigen Test (RAT). For assessment, used a large set of PCR-positive PCR-negative respiratory swabs from asymptomatic symptomatic patients health care workers in setting University...
Double membrane vesicles (DMVs) serve as replication organelles of plus-strand RNA viruses such hepatitis C virus (HCV) and SARS-CoV-2. Viral DMVs are morphologically analogous to formed during autophagy, but lipids driving their biogenesis largely unknown. Here we show that production the lipid phosphatidic acid (PA) by acylglycerolphosphate acyltransferase (AGPAT) 1 2 in ER is important for DMV viral autophagy. Using HCV-replicating cells model, found AGPATs recruited critically contribute...