A5069430515- Acute Myeloid Leukemia Research
- Cancer-related gene regulation
- RNA modifications and cancer
- SARS-CoV-2 and COVID-19 Research
- Epigenetics and DNA Methylation
- Genetic and phenotypic traits in livestock
- Cancer Genomics and Diagnostics
- Reproductive Physiology in Livestock
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Animal Genetics and Reproduction
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Acute Lymphoblastic Leukemia research
- Reproductive Biology and Fertility
- Protein Degradation and Inhibitors
- Reproductive System and Pregnancy
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Biosensors and Analytical Detection
Ludwig-Maximilians-Universität München
2016-2025
LMU Klinikum
2013-2023
Technical University of Munich
2003-2023
Helmholtz Zentrum München
2012-2023
Heinrich Heine University Düsseldorf
2021
Gene Therapy Laboratory
2020
Genetic Analysis (Norway)
2014-2019
German Cancer Research Center
2015-2016
Deutschen Konsortium für Translationale Krebsforschung
2015-2016
Heidelberg University
2015-2016
Abstract A new modification of silver staining is presented which utilizes two chemical properties thiosulfate: image enhancement by pretreatment fixed gels, and formation soluble complexes prevents unspecific background during development. This procedure provides high sensitivity for proteins, RNA DNA in the nanogram range on a colorless, transparent background. The performance this method documented one‐and two‐dimensional patterns plant leaf proteins. Moreover, we achieved, first time,...
Fast and reliable detection of patients with severe heterogeneous illnesses is a major goal precision medicine1,2. Patients leukaemia can be identified using machine learning on the basis their blood transcriptomes3. However, there an increasing divide between what technically possible allowed, because privacy legislation4,5. Here, to facilitate integration any medical data from owner worldwide without violating laws, we introduce Swarm Learning-a decentralized machine-learning approach that...
During maternal-to-embryonic transition control of embryonic development gradually switches from maternal RNAs and proteins stored in the oocyte to gene products generated after genome activation (EGA). Detailed insight into onset transcription is obscured by presence transcripts. Using bovine model system, we established RNA sequencing a comprehensive catalogue transcripts germinal vesicle metaphase II oocytes, embryos at four-cell, eight-cell, 16-cell, blastocyst stages. These were...
Deregulation of Wnt/β-catenin signaling is a hallmark the majority sporadic forms colorectal cancer and results in increased stability protein β-catenin. β-catenin then shuttled into nucleus where it activates transcription its target genes, including proto-oncogenes MYC CCND1 as well genes encoding basic helix-loop-helix (bHLH) proteins ASCL2 ITF-2B. To identify commonly regulated by cell lines, we analyzed gene expression two non-isogenic DLD1 SW480, using DNA microarrays compared these to...
Since autumn 2020, rapid antigen tests (RATs) have been implemented in several countries as an important pillar of the national testing strategy to rapidly screen for infections on site during SARS-CoV-2 pandemic. The current surge infection rates around globe is driven by variant concern (VoC) omicron (B.1.1.529). Here, we evaluated performance nine RATs a single-centre laboratory study. We examined total 115 PCR-negative and 166 PCR-positive respiratory swab samples (101 omicron, 65 delta...
The endometrium plays a central role among the reproductive tissues in context of early embryo–maternal communication and pregnancy. This study investigated transcriptome profiles samples from day 18 pregnant vs non-pregnant heifers to get insight into molecular mechanisms involved conditioning for embryo attachment implantation. Using combination subtracted cDNA libraries array hybridisation, 109 mRNAs with at least twofold higher abundance animals 70 levels control group were identified....
Although somatic cell nuclear transfer (SCNT) cloning is more efficient in cattle than any other species tested so far, there a high rate of pregnancy failure that has been linked to structural and functional abnormalities the placenta. We hypothesis these changes may originate from disturbed embryo-maternal interactions peri-implantation period. Therefore, we evaluated response endometrium SCNT embryos (produced 7 different fetal fibroblast lines) as compared with derived vitro...
Initiation and maintenance of pregnancy are critically dependent on an intact embryo-maternal communication in the preimplantation period. To get new insights into molecular mechanisms underlying this complex dialog, a holistic transcriptome study endometrium samples from Day 18 pregnant vs. nonpregnant twin cows was performed. This genetically defined model system facilitated identification specific conceptus-induced changes transcriptome. Using combination subtracted cDNA libraries array...
ABSTRACT Infections of the udder by Escherichia coli very often elicit acute inflammation, while Staphylococcus aureus infections tend to cause mild, subclinical inflammation and persistent infections. The molecular causes underlying different disease patterns are poorly understood. We therefore profiled kinetics extents global changes in transcriptome primary bovine mammary epithelial cells (MEC) after challenging them with heat-inactivated preparations E. or S. pathogens. swiftly strongly...
Duchenne muscular dystrophy (DMD) is caused by mutations in the X-linked dystrophin gene. The absence of protein leads to progressive muscle weakness and wasting, disability death. To establish a tailored large animal model DMD, we deleted DMD exon 52 male pig cells gene targeting generated offspring nuclear transfer. pigs exhibit skeletal muscles, increased serum creatine kinase levels, dystrophic changes impaired mobility, maximum life span 3 months due respiratory impairment. Unlike human...
The persistent horns are an important trait of speciation for the family Bovidae with complex morphogenesis taking place briefly after birth. polledness is highly favourable in modern cattle breeding systems but serious animal welfare issues urge a solution production hornless other than dehorning. Although dominant inhibition horn was discovered more 70 years ago, and causative mutation mapped almost 20 its molecular nature remained unknown. Here, we report allelic heterogeneity POLLED...
Coliform bacteria are the most common etiologic agents in severe mastitis of cows. Escherichia coli infections mostly restricted to a single udder quarter whereas neighboring quarters stay clinically inapparent, implicating presence systemic defense reaction. To address its underlying mechanism, we performed transcriptome study mammary tissue from inoculated with E. (6 h and 24 post infection), same animals, untreated control animals.After 6 13 probe sets differentially expressed genes (DEG)...