Lorenzo Bonaguro
A5076251093- COVID-19 Clinical Research Studies
- Single-cell and spatial transcriptomics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Blood disorders and treatments
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Immune Cell Function and Interaction
- Long-Term Effects of COVID-19
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Gene expression and cancer classification
- COVID-19 Impact on Reproduction
- Gut microbiota and health
- Neurogenetic and Muscular Disorders Research
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Cell Image Analysis Techniques
- Pancreatic function and diabetes
- Immunotherapy and Immune Responses
- Immune responses and vaccinations
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Bioinformatics and Genomic Networks
University of Bonn
2017-2025
German Center for Neurodegenerative Diseases
2020-2025
Epigenomics (Germany)
2020-2025
University Hospital Bonn
2025
Life & Brain (Germany)
2019
Fast and reliable detection of patients with severe heterogeneous illnesses is a major goal precision medicine1,2. Patients leukaemia can be identified using machine learning on the basis their blood transcriptomes3. However, there an increasing divide between what technically possible allowed, because privacy legislation4,5. Here, to facilitate integration any medical data from owner worldwide without violating laws, we introduce Swarm Learning-a decentralized machine-learning approach that...
Abstract Background The SARS-CoV-2 pandemic is currently leading to increasing numbers of COVID-19 patients all over the world. Clinical presentations range from asymptomatic, mild respiratory tract infection, severe cases with acute distress syndrome, failure, and death. Reports on a dysregulated immune system in call for better characterization understanding changes system. Methods In order dissect COVID-19-driven host responses, we performed RNA-seq whole blood cell transcriptomes...
Severe COVID-19 is linked to both dysfunctional immune response and unrestrained immunopathology, it remains unclear whether T cells contribute disease pathology. Here, we combined single-cell transcriptomics proteomics with mechanistic studies assess pathogenic cell functions inducing signals. We identified highly activated CD16
Objective Chronic obstructive pulmonary disease (COPD) is a major cause of global illness and death, most commonly caused by cigarette smoke. The mechanisms pathogenesis remain poorly understood, limiting the development effective therapies. gastrointestinal microbiome has been implicated in chronic lung diseases via gut-lung axis, but its role unclear. Design Using an vivo mouse model smoke (CS)-induced COPD faecal microbial transfer (FMT), we characterised microbiota using metagenomics,...
Systemic inflammation is established as part of late-stage severe lung disease, but molecular, functional, and phenotypic changes in peripheral immune cells early disease stages remain ill defined. Chronic obstructive pulmonary (COPD) a major respiratory characterized by small-airway inflammation, emphysema, breathing difficulties. Using single-cell analyses we demonstrate that blood neutrophils are already increased early-stage COPD, molecular functional neutrophil states correlate with...
Dexamethasone is a life-saving treatment for severe COVID-19, yet its mechanism of action unknown, and many patients deteriorate or die despite timely initiation. Here, we identify dexamethasone treatment-induced cellular molecular changes associated with improved survival in COVID-19 patients. We observed reversal transcriptional hallmark signatures monocytes the induction monocyte substate characterized by expression glucocorticoid-response genes. These responses to were detected...
Whether neurodevelopmental defects underlie postnatal neuronal death in neurodegeneration is an intriguing hypothesis only recently explored. Here, we focus on spinal muscular atrophy (SMA), a neuromuscular disorder caused by reduced survival of motor neuron (SMN) protein levels leading to (MN) loss and muscle wasting. Using the first isogenic patient-derived induced pluripotent stem cell (iPSC) model cord organoid (SCO) system, show that SMA SCOs exhibit abnormal morphological development,...
Despite its high prevalence, the cellular and molecular mechanisms of chronic obstructive pulmonary disease (COPD) are far from being understood. Here, we determine disease-related changes in compositions within alveolar space peripheral blood a cohort COPD patients controls. Myeloid cells were largest compartment with invading monocytes proliferating macrophages elevated COPD. Modeling cell-to-cell communication, signaling pathway usage, transcription factor binding predicts TGF-β1 to be...
Abstract ‘Severe Acute Respiratory Syndrome - Coronavirus-2’ (SARS-CoV-2) infection causes Coronavirus Disease 2019 (COVID-19), a mild to moderate respiratory tract in the majority of patients. A subset patients, however, progresses severe disease and failure with acute distress syndrome (ARDS). Severe COVID-19 has been associated increased neutrophil counts dysregulated immune responses. The mechanisms protective immunity forms pathogenesis inflammation courses remain largely unclear. Here,...
The unfolded protein response (UPR) is associated with hepatic metabolic function, yet it not well understood how endoplasmic reticulum (ER) disturbance might influence homeostasis. Here, we describe the physiological function of Cysteine-rich EGF-like domains 2 (Creld2), previously characterized as a downstream target ER-stress signal transducer Atf6. To this end, generated Creld2-deficient mice and induced UPR by injection tunicamycin. Creld2 augments folding creates an interlink between...
Introduction People living with HIV (PLHIV) are characterized by functional reprogramming of innate immune cells even after long-term antiretroviral therapy (ART). In order to assess technical feasibility omics technologies for application larger cohorts, we compared multiple data layers. Methods Bulk and single-cell transcriptomics, flow cytometry, proteomics, chromatin landscape analysis ATAC-seq as well ex vivo drug stimulation were performed in a small number blood samples derived from...
During perinatal development, liver metabolism is tightly regulated to ensure energy supply for the newborn. Before birth, glycogen stored in hepatocytes and later metabolized glucose, meeting demands of neonate. Shortly after lipogenesis begins, driven by transcriptional activation enzymes involved fatty acid oxidation. These processes are thought be largely systemic insulin glucagon levels. However, role liver-derived local factors neonatal hepatocyte remains unexplored. Kupffer cells...
In central nervous system (CNS) diseases characterized by late-onset neurodegeneration, the interplay between innate and adaptive immune responses remains poorly understood. This knowledge gap is exacerbated prolonged protracted disease course as it complicates delineation of brain-resident infiltrating cells. Here, we conducted comprehensive profiling cells in a murine model spastic paraplegia 15 (SPG15), complicated form hereditary paraplegia. Using fate-mapping bone marrow–derived cells,...
SUMMARY The SARS-CoV-2 pandemic is currently leading to increasing numbers of COVID-19 patients all over the world. Clinical presentations range from asymptomatic, mild respiratory tract infection, severe cases with acute distress syndrome, failure, and death. Reports on a dysregulated immune system in calls for better characterization understanding changes system. Here, we profiled whole blood transcriptomes 39 10 control donors enabling data-driven stratification based molecular phenotype....
In December 2019, a new coronavirus, SARS-CoV-2, which causes the respiratory illness that led to COVID-19 pandemic, was reported. face of such pathogen, special precautions must be taken examine potentially infectious materials due lack knowledge on disease transmissibility, infectivity, and molecular pathogenicity. Here, we present complete safe workflow for performing scRNA-seq experiments blood samples infected patients from cell isolation data analysis using micro-well based BD Rhapsody...
Abstract High-dimensional cytometry (HDC) is a powerful technology for studying single-cell phenotypes in complex biological systems. Although technological developments and affordability have made HDC broadly available recent years, advances were not coupled with an adequate development of analytical methods that can take full advantage the data generated. While several platforms bioinformatics tools become analysis data, these are either web-hosted limited scalability or designed expert...
CRELD1 (Cysteine-Rich with EGF-Like Domains 1) is a risk gene for non-syndromic atrioventricular septal defects in human patients. In mouse model, Creld1 has been shown to be essential heart development, particularly septum and valve formation. However, due the embryonic lethality of global knockout (KO) mice, its cell type-specific function during peri- postnatal stages remains unknown.Here, we generated conditional KO mice lacking either endocardium (KOTie2) or myocardium (KOMyHC). Using...