A5027680282- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Advanced Proteomics Techniques and Applications
- Eosinophilic Esophagitis
- Endoplasmic Reticulum Stress and Disease
- IL-33, ST2, and ILC Pathways
- Diet, Metabolism, and Disease
- Pancreatic function and diabetes
- Peroxisome Proliferator-Activated Receptors
- Sphingolipid Metabolism and Signaling
- Click Chemistry and Applications
- Diet and metabolism studies
- Birth, Development, and Health
- Advanced Biosensing Techniques and Applications
- Neonatal Respiratory Health Research
- Epigenetics and DNA Methylation
- Metabolism, Diabetes, and Cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
University of Bonn
2016-2024
Triglyceride cycling is the process of continuous degradation and re-synthesis triglyceride in cellular stores. We show 3T3-L1 adipocytes that triglycerides are subject to rapid turnover re-arrangement fatty acids with an estimated half-life 2-4 h. develop a tracing technology can simultaneously quantitatively follow metabolism multiple study futile substrate cycle directly molecular species resolution. Our approach based on alkyne acid tracers mass spectrometry. The connected modification...
1-Deoxysphingolipids (deoxySLs) are atypical sphingolipids that elevated in the plasma of patients with type 2 diabetes and hereditary sensory autonomic neuropathy 1 (HSAN1). Clinically, diabetic HSAN1 very similar, suggesting involvement deoxySLs pathology both diseases. However, little is known about biology these lipids underlying pathomechanism. We synthesized an alkyne analog 1-deoxysphinganine (doxSA), metabolic precursor all deoxySLs, to trace metabolism localization deoxySLs. Our...
The unfolded protein response (UPR) is associated with hepatic metabolic function, yet it not well understood how endoplasmic reticulum (ER) disturbance might influence homeostasis. Here, we describe the physiological function of Cysteine-rich EGF-like domains 2 (Creld2), previously characterized as a downstream target ER-stress signal transducer Atf6. To this end, generated Creld2-deficient mice and induced UPR by injection tunicamycin. Creld2 augments folding creates an interlink between...
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor regulating xenobiotic responses as well physiological metabolism. Dietary AhR ligands activate the signaling axis, whereas activation negatively regulated by repressor (AhRR). While AhR-deficient mice are known to be resistant diet-induced obesity (DIO), influence of AhRR on DIO has not been assessed so far. In this study, we analyzed AhRR-/- and with conditional deletion either or in myeloid cells under...
Summary Cellular heterogeneity is a fundamental facet of cell biology, influencing cellular signaling, metabolism, and gene regulation. Its accurate quantification requires measurements at the single-cell level. Most high-throughput technologies provide only snapshot specific time point because measurement destructive. This limits our current ability to understand dynamics behavior quantify cell-specific parameters. We propose an experimental setup combined with model-based analysis...
Insulin is a peptide hormone that secreted in Golgi-derived dense-core vesicles from mammalian pancreatic beta-cells response to nutrients. In Drosophila melanogaster, three insulin-like peptides are as neuropeptides the insulin-producing cells brain. Peroxisomes lipid-metabolizing organelles engage into various membrane contact sites with other organelles. Impaired peroxisomal metabolism has been associated beta-cell apoptosis and impaired insulin secretion. How peroxisomes contribute...
Medium-chain fatty acids (MCFAs) play an increasing role in human nutrition. In the liver, one fraction is used for synthesis of MCFA-containing triacylglycerol (MCFA-TG), and rest oxidative energy production or ketogenesis. We investigated which enzymes catalyse MCFA-TG how inhibition acid (FA) oxidation influences metabolic fate MCFAs.FA metabolism was followed by time-resolved tracing alkyne-labelled FAs freshly isolated mouse hepatocytes. Quantitative data were obtained mass spectrometry...
<title>Abstract</title> Kupffer cells (KCs) are tissue-resident macrophages which colonize the developing liver early during embryogenesis<sup>1</sup>. Throughout development and adulthood, KCs have distinct core functions that essential for organismal homeostasis, such as supporting fetal erythropoiesis well postnatal erythrocyte recycling metabolism<sup>2</sup>. start to acquire their tissue-specific transcriptional signature immediately after colonizing liver, mature together with tissue,...
Abstract The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor regulating xenobiotic responses as well physiological metabolism. Dietary AhR ligands activate the signaling axis in intestine and throughout organism, whereas activation negatively regulated by repressor (AhRR). While AhR-deficient mice are known to be resistant diet-induced obesity (DIO), we here demonstrate that AhRR deficiency also leads robust, but not profound protection from DIO hepatosteatosis....
Abstract The unfolded protein response (UPR) is associated with the hepatic metabolic function, yet it not well understood how endoplasmic reticulum (ER) disturbance might influence homeostasis. Here, we describe physiological function of Cysteine-rich EGF-like domains 2 (Creld2), previously characterized as a downstream target ER-stress signal transducer Atf6. To this end generated Creld2 -deficient mice and induced UPR by injection tunicamycin. augments folding creates an interlink between...
Abstract This protocol provides a method for sensitive multiplexed metabolic tracing. It is based on the combination of alkyne-labeled precursors and dedicated azido reporter molecules that are optimized mass spectrometric detection after click reaction. After incorporation alkyne fatty acids, sub-picomole to femtomole amounts labeled material can be detected. Time resolution achieved by pulse-chase labeling.