A5009888436- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and biosynthesis
- Advanced Proteomics Techniques and Applications
- Genetic and Kidney Cyst Diseases
- Click Chemistry and Applications
- Mass Spectrometry Techniques and Applications
- Phagocytosis and Immune Regulation
- Peroxisome Proliferator-Activated Receptors
- Metabolism and Genetic Disorders
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- Immune cells in cancer
- Cholesterol and Lipid Metabolism
- Neurobiology and Insect Physiology Research
- Adenosine and Purinergic Signaling
University of Bonn
2017-2025
Dana-Farber Cancer Institute
2024
The primary cilium constitutes an organelle that orchestrates signal transduction independently from the cell body. Dysregulation of this intricate molecular architecture leads to severe human diseases, commonly referred as ciliopathies. However, underpinnings how ciliary signaling a specific cellular output remain elusive. By combining spatially resolved optogenetics with RNA sequencing and imaging, we reveal novel cAMP signalosome is functionally distinct cytoplasm. We identify genes...
The different adipose tissues (ATs) can be distinguished according to their function. For example, white AT stores energy in form of lipids, whereas brown dissipates the heat. These functional differences are represented respective adipocyte morphology; adipocytes contain large, unilocular lipid droplets, smaller, multilocular droplets. However, an automated, image analysis pipeline comprehensively analyze vitro cell culture as well ex vivo tissue sections is missing. We here present AdipoQ,...
Promoting brown adipose tissue (BAT) activity innovatively targets obesity and metabolic disease. While thermogenic activation of BAT is well understood, the rheostatic regulation to avoid excessive energy dissipation remains ill-defined. Here, we demonstrate that adenylyl cyclase 3 (AC3) key for function. We identified a cold-inducible promoter generates 5′ truncated AC3 mRNA isoform (Adcy3-at), whose expression driven by cold-induced, PPARGC1A (PPARGC1A-AT). Male mice lacking Adcy3-at...
Fatty acid beta-oxidation is a key process in mammalian lipid catabolism. Disturbance of this results severe clinical symptoms, including dysfunction the liver, major beta-oxidizing tissue. For thorough understanding process, comprehensive analysis involved fatty and acyl-carnitine intermediates desired, but capable methods are lacking. Here, we introduce oxaalkyne alkyne acids as novel tracers to study long- medium-chain liver lysates primary hepatocytes. Combining these new tracer tools...
Abstract Macrophages engulf apoptotic bodies and cellular debris as part of homeostasis, but they can also phagocytose live cells such aged red blood cells. Pharmacologic reprogramming with the SMAC mimetic LCL161 in combination T cell-derived cytokines induce macrophages to cancer mouse models. Here we extend these findings encompass a wide range monovalent bivalent compounds, demonstrating that cell phagocytosis is class effect agents. We demonstrate robust pancreatic breast by primary...
Abstract This protocol provides a method for sensitive multiplexed metabolic tracing. It is based on the combination of alkyne-labeled precursors and dedicated azido reporter molecules that are optimized mass spectrometric detection after click reaction. After incorporation alkyne fatty acids, sub-picomole to femtomole amounts labeled material can be detected. Time resolution achieved by pulse-chase labeling.
Abstract The different adipose tissues can be distinguished according to their function. For example, white tissue (WAT) stores energy in form of lipids, whereas brown (BAT) dissipates the heat. These functional differences are represented respective adipocyte morphology: adipocytes contain large, unilocular lipid droplets, smaller, multilocular droplets. However, an automated, image-analysis pipeline comprehensively analyze vitro cell culture as well ex vivo sections is missing. We here...
Abstract Promoting brown adipose tissue (BAT) activity has been recognized as innovative therapeutic approach to improve obesity and metabolic disease. Whilst the molecular circuitry underlying thermogenic activation of BAT is well understood, processes rheostatic regulation maintain homeostasis avoid excessive energy dissipation remain ill-defined. Increasing cyclic AMP (cAMP) biosynthesis key for activation. Here, we demonstrate that ADCY3, an adenylyl cyclase whose expression induced...