A5076499211- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Insect and Arachnid Ecology and Behavior
- Physiological and biochemical adaptations
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Molecular Communication and Nanonetworks
- Circadian rhythm and melatonin
- Animal Behavior and Reproduction
- Sleep and Wakefulness Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Development and Disorders
- Insect Utilization and Effects
- Spectroscopy and Quantum Chemical Studies
- Cellular transport and secretion
- Cholesterol and Lipid Metabolism
- Adenosine and Purinergic Signaling
- Lipid metabolism and biosynthesis
- Insect and Pesticide Research
- Insect Resistance and Genetics
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
University of Washington
2024
Universität Hamburg
2017-2022
University Medical Center Hamburg-Eppendorf
2017-2022
Abstract Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control allowing both excitation inhibition the same neurons a single experiment is desired. This requires low spectral overlap between opsin, matched photocurrent amplitudes fixed expression ratio. Moreover, independent activation two distinct populations with different optogenetic...
Abstract Genetic engineering of natural light-gated ion channels has proven a powerful way to generate optogenetic tools for wide variety applications. In recent years, blue-light activated engineered anion-conducting channelrhodopsins (eACRs) have been developed, improved, and were successfully applied in vivo . We asked whether the approaches used create eACRs can be transferred other well-characterized cation-conducting (CCRs) obtain with broad spectrum biophysical properties. generated...
Many neurodegenerative disorders (NDDs) preferentially affect neurons with long or complex axonal arbors but the cellular and molecular bases for neurite length-dependent vulnerability of to degeneration is largely unknown. Using Drosophila sensory as a model system we show that neuronal activation integrated stress response triggers expression Interleukin-6 homolog unpaired 3 ( upd3 ), which both necessary sufficient axon length–dependent presynapses. Upd3 activates phagocytic glia,...
Abstract Sensory circuits are typically established during early development, yet how circuit specificity and function maintained organismal growth has not been elucidated. To gain insight we quantitatively investigated synaptic connectivity in the Drosophila nociceptive network larval development. We show that between primary nociceptors their downstream neurons scales with animal size. further identified conserved Ste20-like kinase Tao as a negative regulator of required for maintenance...
Dendritic arborization is highly regulated and requires tight control of dendritic growth, branching, cytoskeletal dynamics, ion channel expression to ensure proper function. Abnormal development can result in altered network connectivity, which has been linked neurodevelopmental disorders, including autism spectrum disorders (ASDs). How neuronal growth programs tune function still not fully understood. Using Drosophila (da) neurons as a model, we identified the conserved Ste20-like kinase...
Abstract Optogenetic silencing allows to reveal the necessity of selected neuronal populations for various neurophysiological functions. These range from synaptic transmission and coordinated network activity control specific behaviors. An ideal single-component optogenetic tool should be switchable between active inactive states with precise timing while preserving its in absence light until switched an state. Although bistable anion-conducting channelrhodopsins (ACRs) were previously...
Drosophila melanogaster larvae have been extensively used as a model to study the molecular and cellular basis of nociception. The larval nociceptors, class IV dendritic arborization (C4da) neurons, line body wall animal respond various stimuli including noxious heat touch. Activation C4da neurons results in stereotyped escape behavior, characterized by 360° rolling response along axis followed locomotion speedup. genetic accessibility has allowed identification mechanosensory channels...
Abstract Animals display a plethora of escape behaviors when faced with environmental threats. Selection the appropriate response by underlying neuronal network is key to maximize chances survival. We uncovered somatosensory in Drosophila larvae that encodes two through input-specific neuropeptide action. Sensory neurons required for avoidance noxious light and harsh touch, each converge on discrete domains same neuromodulatory hub neurons. These gate touch responses via short Neuropeptide...
Thermo-nociception, the detection and behavioral response to noxious temperatures, is a highly conserved action avoid injury ensure survival. Basic molecular mechanisms of thermal responses have been elucidated in several model organisms are clinical relevance as hypersensitivity (thermos-allodynia) common neuropathic pain syndromes. Drosophila larvae show stereotyped escape behavior upon heat stimulation, which can be easily quantified coupled with genetic approaches. It has successfully...
Genetic engineering of natural light-gated ion channels has proven a powerful way to generate optogenetic tools for wide variety applications. In recent years, blue light-activated engineered anion conducting channelrhodopsins (eACRs) have been developed, improved, and were successfully applied in vivo . We asked whether the approaches used create eACRs can be transferred other well-characterized cation-conducting (CCRs) obtain with broad spectrum biophysical properties. generated 22...
Abstract Many neurodegenerative disorders (NDDs) preferentially affect neurons with long or complex axonal arbors, but our understanding of this specific vulnerability is limited. Using Drosophila larval class IV dendrite arborization (C4da) neurons, we found that neuronal activation the integrated stress response (ISR) induces axon length-dependent degeneration (LDD). We identified Interleukin-6 homologue unpaired 3 (upd3) as both necessary and sufficient for LDD in C4da neurons. Upd3...
Abstract Optogenetic silencing allows to reveal the necessity of selected neuronal populations for various neurophysiological functions ranging from specific behaviors network activity. An ideal optogenetic tool should be switchable between active and inactive states with precise timing while preserving its activity in absence light until switched an state. Although bistable anion-conducting channelrhodopsins (ACRs) were previously engineered reach this goal, their conducting state life time...
Abstract Sensory circuits are typically established during early development, yet how circuit specificity and function maintained organismal growth has not been elucidated. To gain insight we quantitatively investigated synaptic connectivity in the Drosophila nociceptive network larval development. We show that between primary nociceptors their downstream neurons scales with animal size. further identified conserved Ste20-like kinase Tao as a negative regulator of required for maintenance...