A5049125747- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Pain Mechanisms and Treatments
- Blood properties and coagulation
- Ion Channels and Receptors
- Genetics, Aging, and Longevity in Model Organisms
- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
University Medical Center Hamburg-Eppendorf
2022-2024
Universität Hamburg
2022-2024
Goethe University Frankfurt
2020-2023
Abstract Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control allowing both excitation inhibition the same neurons a single experiment is desired. This requires low spectral overlap between opsin, matched photocurrent amplitudes fixed expression ratio. Moreover, independent activation two distinct populations with different optogenetic...
PIEZO1 and PIEZO2 are mechanically activated ion channels that confer mechanosensitivity to various cell types. PIEZO commonly examined using the so-called poking technique, where currents recorded in whole-cell configuration of patch-clamp while surface is stimulated with a small fire-polished patch pipette. Currently, there no gold standard for mechanical stimulation, therefore, stimulation protocols differ significantly between laboratories regard velocity, angle, size probe. Here, we...
Excitable cells can be stimulated or inhibited by optogenetics. Since optogenetic actuation regimes are often static, neurons and circuits quickly adapt, allowing perturbation, but not true control. Hence, we established an voltage-clamp (OVC). The voltage-indicator QuasAr2 provides information for fast, closed-loop optical feedback to the bidirectional actuator BiPOLES. Voltage-dependent fluorescence is held within tight margins, thus clamping cell distinct potentials. We OVC in muscles of...
PKA is a downstream effector of many inflammatory mediators that induce pain hypersensitivity by increasing the mechanosensitivity nociceptive sensory afferent. Here, we examine molecular mechanism underlying PKA-dependent modulation mechanically activated ion channel PIEZO2, which confers to nociceptors. Using phosphorylation site prediction algorithms, identified multiple putative and highly conserved sites located on intracellular intrinsically disordered regions PIEZO2. Site-directed...
Abstract Mechanically silent nociceptors are sensory afferents that insensitive to noxious mechanical stimuli under normal conditions but become sensitized such during inflammation. Using RNA-sequencing and quantitative RT-PCR we demonstrate inflammation upregulates the expression of transmembrane protein TMEM100 in electrophysiology revealed over-expression is required sufficient un-silence mice. Moreover, show mice lacking do not develop secondary hypersensitivity—i.e., pain...
Abstract Optogenetic manipulation of neuronal activity has become an indispensable experimental strategy in neuroscience research. A large repertoire excitatory and inhibitory tools allows precise activation or inhibition genetically targetable populations. However, optogenetic tool for reliable bidirectional control allowing both up- downregulation the same neurons a single experiment is still missing. Here we report BiPOLES, potent excitation population with light two different colors....
Abstract Excitable cells can be stimulated or inhibited by optogenetics. Since optogenetic actuation regimes are often static, neurons and circuits quickly adapt, allowing perturbation, but not true control. Hence, we established an voltage-clamp (OVC). The voltage-indicator QuasAr2 provides information for fast, closed-loop optical feedback to the bidirectional actuator BiPOLES. Voltage-dependent fluorescence is held within tight margins, thus clamping cell distinct potentials. We OVC in...
Summary Silent nociceptors are sensory afferents that insensitive to noxious mechanical stimuli under normal conditions but become sensitized such during inflammation. Using RNA-sequencing and quantitative RT-PCR we demonstrate inflammation selectively upregulates the expression of transmembrane protein TMEM100 in silent electrophysiology revealed over-expression is required sufficient un-silence nociceptors. Moreover, show mice lacking do not develop secondary allodynia – i.e. pain...
Abstract The mechanically activated ion channel PIEZO1 confers mechanosensitivity to many cell types and is thus essential for numerous physiological processes triggered by mechanical cues 1–4 . channels assemble as propeller-shaped trimers 5–7 , which supposedly undergo a transition from curved conformational state into flattened upon activation 8–11 Whether this gating model also applies clusters what extent membrane curvature alters conformation in living cells, is, however, still...
Abstract Excitable cells can be stimulated or inhibited by optogenetics. Since optogenetic actuation regimes are often static, neurons and circuits quickly adapt, allowing perturbation, but not true control. Hence, we established an voltage-clamp (OVC). The voltage-indicator QuasAr2 provides information for fast, closed-loop optical feedback to the bidirectional actuator BiPOLES. Voltage-dependent fluorescence is held within tight margins, thus clamping cell distinct potentials. We OVC in...