A5022310730- Immune responses and vaccinations
- Diabetes and associated disorders
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Adipokines, Inflammation, and Metabolic Diseases
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Antifungal resistance and susceptibility
- Multiple Myeloma Research and Treatments
- Cytokine Signaling Pathways and Interactions
- IL-33, ST2, and ILC Pathways
- Dietary Effects on Health
- Rheumatoid Arthritis Research and Therapies
- Protein Degradation and Inhibitors
- Diet and metabolism studies
- Nutritional Studies and Diet
- Inflammasome and immune disorders
- Sleep and Wakefulness Research
- Autophagy in Disease and Therapy
- Immune cells in cancer
- Fungal Infections and Studies
- RNA modifications and cancer
- interferon and immune responses
- Cardiovascular Disease and Adiposity
- COVID-19 Impact on Reproduction
CeMM Research Center for Molecular Medicine
2021-2024
Radboud University Nijmegen
2015-2024
Radboud University Medical Center
2015-2024
Austrian Academy of Sciences
2021-2024
University Medical Center
2016-2023
Radboud Institute for Molecular Life Sciences
2015-2022
Médecins Sans Frontières
2011
Immune responses are tightly regulated yet highly variable between individuals. To investigate human population variation of trained immunity, we immunized healthy individuals with Bacillus Calmette-Guérin (BCG). This live-attenuated vaccine induces not only an adaptive immune response against tuberculosis but also triggers innate activation and memory that indicative immunity. We established personal profiles chromatin accessibility maps over a 90-day time course BCG vaccination in 323 Our...
Quantitative microscopy has proven a versatile and powerful phenotypic screening technique. Recently, image-based profiling shown promise as means for broadly characterizing molecules' effects on cells in several drug-discovery applications, including target-agnostic predicting compound's mechanism of action (MOA). Several methods have been proposed, but little is known about their comparative performance, impeding the wider adoption further development profiling. We compared these by...
Metabolic dysregulation and inflammation are important consequences of obesity impact susceptibility to cardiovascular disease. Anti-inflammatory therapy in disease is being developed under the assumption that inflammatory pathways identical women men, but it not known if this indeed case. In study, we assessed sex-specific relation between metabolic obesity. Approach Results: Three hundred two individuals were included, half with a BMI 27 30 kg/m2 BMI>30 kg/m2, 45% women. The presence...
Abstract In response to infection, macrophages adapt their metabolism rapidly enhance glycolysis and fuel specialized antimicrobial effector functions. Here we show that fungal melanin is an essential molecule required for the metabolic rewiring of during infection with pathogen Aspergillus fumigatus . Using pharmacological genetic tools, reveal a molecular link between calcium sequestration by inside phagosome induction efficient innate immune responses. By remodeling intracellular...
Bile acids (BAs) are implicated in the etiology of obesity-related conditions such as non-alcoholic fatty liver disease. Differently structured BA species display variable signaling activities via farnesoid X receptor (FXR) and Takeda G protein-coupled 1 (TGR5). This study profiles plasma fecal BAs 7α-hydroxy-4-cholesten-3-one (C4) 297 persons with obesity, identifies underlying genetic microbial determinants, establishes correlations fat lipid parameters. We identify 27 associations (p < 5...
Due to unacceptably high mortality with pentavalent antimonials, Médecins Sans Frontières in 2006 began using liposomal amphotericin B (AmBisome) for visceral leishmaniasis (VL) patients Ethiopia who were severely ill or positive human immunodeficiency virus (HIV).We used clinical data obtained from January 2007 2009 compare outcomes by HIV status and VL episode (primary vs relapse) identify risk factors treatment failure among treated AmBisome monotherapy at a total dose of 30 mg/kg 6 doses...
Monocytes are innate immune cells that play a pivotal role in antifungal immunity, but little is known regarding the cellular metabolic events regulate their function during infection. Using complementary transcriptomic and immunological studies human primary monocytes, we show activation of monocytes by Candida albicans yeast hyphae was accompanied rewiring induced through C-type lectin-signaling pathways. We describe responses against energy-demanding processes lead to mobilization...
Chronic inflammation and immune dysfunction play a key role in the development of non-AIDS–related comorbidities. The aim our study was to characterize functional phenotype cells people living with HIV (PLHIV). We enrolled cross-sectional cohort PLHIV on stable antiretroviral therapy healthy controls. assessed ex vivo cytokine production capacity transcriptomics monocytes T upon bacterial, fungal, viral stimulation. exhibited an exacerbated proinflammatory profile monocyte-derived cytokines,...
Dietary patterns have been associated with variations in behavior. However, evidence has limited and mixed, the underlying mechanism remains unclear.Extend a previous study reporting significant associations between food behavioral disinhibition explore whether low-grade inflammation is linked to behaviors mediates association diet disinhibition.Among participants of UK Biobank (UKB) we extracted single principal component using UKB touchscreen questionnaire, Mental Health Questionnaire...
Abstract Dietary habits may affect inflammatory status in humans. Here we explore this interaction as well the potential mediating role of gut microbiome (GM), given that GM is both involved processing dietary components and influences immune system. A cross-sectional analysis a sample 482 healthy participants (207 males 275 females) was performed. intake assessed by semiquantitative food questionnaire. Adipokines soluble mediators were assayed with multiple immunoassays ELISA. Microbial DNA...
Abstract Polysomnography (PSG) is the gold standard for recording sleep. However, PSG systems are bulky, expensive, and often confined to lab environments. These also time-consuming in electrode placement sleep scoring. Such limitations render less suitable large-scale or longitudinal studies of Recent advances electronics artificial intelligence enabled ‘wearable’ systems. Here, we present a study aimed at validating performance ZMax, widely-used wearable that includes frontal...
Abstract Secondary infections are a major complication of sepsis and associated with compromised immune state, called sepsis-induced immunoparalysis. Molecular mechanisms causing immunoparalysis remain unclear; however, changes in cellular metabolism leukocytes have been linked to We investigated the relation metabolic antimicrobial monocyte functions endotoxin-induced immunotolerance, as model for In this study, immunotolerance was induced healthy males by intravenous endotoxin (2 ng/kg,...
Abstract Immunopathology contributes to high mortality in tuberculous meningitis (TBM) but little is known about the blood and cerebrospinal fluid (CSF) immune response. We prospectively characterised response of 160 TBM suspects an Indonesian cohort, including 67 HIV-negative probable or definite cases. patients presented with severe disease 38% died 6 months. Blood from analysed by flow cytometry showed lower αβT γδT cells, NK cells MAIT compared 26 pulmonary tuberculosis (2.4-4-fold, all...
The IL-1 family member IL-38 (IL1F10) suppresses inflammatory and autoimmune conditions. Here, we report that plasma concentrations of in 288 healthy Europeans correlate positively with circulating memory B cells plasmablasts. correlated negatively age (p = 0.02) was stable 48 subjects for 1 year. In comparison primary keratinocytes, IL1F10 expression CD19+ from PBMC lower, whereas cell-associated comparable. vitro, is released after stimulation rituximab. Intravenous LPS humans failed to...
Different components of the immune response show large variability between individuals, but they also vary within same individual because host and environmental factors. In this study, we report an extensive analysis characteristics 56 individuals over four timepoints in 1 single year as part Human Functional Genomics Project. We characterized 102 cell subsets using flow cytometry; quantified production eight cytokines two chemokines to 20 metabolic, bacterial, fungal, viral stimuli;...