A5044137556- HIV Research and Treatment
- HIV-related health complications and treatments
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Cytomegalovirus and herpesvirus research
- Stress Responses and Cortisol
- Tryptophan and brain disorders
- Maternal Mental Health During Pregnancy and Postpartum
- Pharmacological Effects and Toxicity Studies
- Diet and metabolism studies
- Diabetes Management and Research
- Neuroendocrine regulation and behavior
- Viral-associated cancers and disorders
- T-cell and B-cell Immunology
- Systemic Lupus Erythematosus Research
- Phagocytosis and Immune Regulation
- Malaria Research and Control
- Metabolomics and Mass Spectrometry Studies
- Mosquito-borne diseases and control
- Diabetes Management and Education
- GDF15 and Related Biomarkers
- Influenza Virus Research Studies
- Acute Myeloid Leukemia Research
Radboud University Nijmegen
2016-2023
Radboud University Medical Center
2016-2023
Chronic inflammation and immune dysfunction play a key role in the development of non-AIDS–related comorbidities. The aim our study was to characterize functional phenotype cells people living with HIV (PLHIV). We enrolled cross-sectional cohort PLHIV on stable antiretroviral therapy healthy controls. assessed ex vivo cytokine production capacity transcriptomics monocytes T upon bacterial, fungal, viral stimulation. exhibited an exacerbated proinflammatory profile monocyte-derived cytokines,...
Summary Severe dengue is characterised by thrombocytopenia, plasma leakage and bleeding. Platelets are important for preservation of endothelial integrity. We hypothesised that platelet activation with secondary dysfunction contribute to leakage. In adult Indonesian patients acute dengue, we measured status the response agonist TRAP using flow cytometerbased assays. Patients were monitored daily ultrasonography. Acute was associated an increased expression activated fibrinogen receptor...
Different components of the immune response show large variability between individuals, but they also vary within same individual because host and environmental factors. In this study, we report an extensive analysis characteristics 56 individuals over four timepoints in 1 single year as part Human Functional Genomics Project. We characterized 102 cell subsets using flow cytometry; quantified production eight cytokines two chemokines to 20 metabolic, bacterial, fungal, viral stimuli;...
Abstract Clonal hematopoiesis, a common age-related phenomenon marked by expansion of cells with clonal hematopoiesis driver mutations, has been associated all-cause mortality, cancer, and cardiovascular disease. People HIV (PWH) are at risk for non-AIDS–related comorbidities such as atherosclerotic disease cancer. In cross-sectional cohort study, we compared prevalence in PWH on stable antiretroviral therapy overweight individuals age- sex-matched population controls. The adjusted age was...
Abstract HIV infection and antiretroviral therapy have been linked to mitochondrial dysfunction. The role of platelet dysfunction in thrombosis, immunoregulation age-related diseases is increasingly appreciated. Here, we studied DNA content (mtDNA pl ) function people living with (PLHIV) related this function. In a cohort 208 treated PLHIV 56 uninfected controls, mtDNA was quantified, as well activation, agonist-induced reactivity inflammation by circulating factors flow cytometry. subgroup...
Long-term changes in the immune system of successfully treated people living with HIV (PLHIV) remain incompletely understood. In this study, we assessed 108 white blood cell (WBC) populations a cohort 211 PLHIV on stable antiretroviral therapy and 56 HIV-uninfected controls using flow cytometry. We show that marked differences exist T maturation differentiation between controls: had reduced percentages CD4+ cells naïve increased CD8+ cells, effector helper 17 (Th17) together Th17/regulatory...
Introduction People living with HIV (PLHIV) are characterized by functional reprogramming of innate immune cells even after long-term antiretroviral therapy (ART). In order to assess technical feasibility omics technologies for application larger cohorts, we compared multiple data layers. Methods Bulk and single-cell transcriptomics, flow cytometry, proteomics, chromatin landscape analysis ATAC-seq as well ex vivo drug stimulation were performed in a small number blood samples derived from...
Abstract Efavirenz is recommended as a preferred first-line drug for women of childbearing potential living with human immunodeficiency virus. known its central nervous system side effects, which are partly mediated by serotonergic actions. The neurotransmitter serotonin exerts neurotrophic effects during neurodevelopment and antenatal exposure to agents has been linked developmental delay. Although the teratogenic risks efavirenz appear be minimal, data on long-term remain scarce. Here, we...
BACKGROUNDPeople living with HIV (PLHIV) receiving antiretroviral therapy (ART) exhibit persistent immune dysregulation and microbial dysbiosis, leading to development of cardiovascular diseases (CVDs). We initially compared plasma proteomic profiles between 205 PLHIV 120 healthy control participants (HCs) validated the results in an independent cohort 639 99 HCs. Differentially expressed proteins (DEPs) were then associated microbiome data. Finally, we assessed which linked CVD...
People living with HIV (PLHIV) exhibit dysregulation of tryptophan metabolism. Altered gut microbiome composition in PLHIV might be involved. Mechanistic consequences within the 3 major metabolism pathways (serotonin, kynurenine, and indoles), functional for platelet, immune behavioral functions are unknown. We investigated plasma metabolites, composition, their association platelet function, inflammation, psychiatric symptoms.
To investigate whether a history of severe hypoglycaemia (SH) or the associated presence impaired awareness (IAH) is characterized by pro-inflammatory profile in people with type 1 diabetes.
Psychiatric symptoms are prevalent in people living with HIV (PLWH), especially depression, anxiety, impulsivity, and substance use. Various biological mechanisms might play a role the occurrence of psychiatric this population. A hypothesis free, data-driven metabolomics approach can further our understanding these mechanisms. In study, we identified metabolic pathways associated depression use 157 PLWH. First, Spearman's rank correlations between metabolite feature intensities symptom...
Background: D-dimer concentrations in people living with HIV (PLHIV) on combination antiretroviral therapy (cART) are increased and have been linked to mortality. is a biomarker of vivo coagulation. In contrast reports D-dimer, data coagulation capacity PLHIV conflicting. this study, we assessed the effect cART inflammation capacity. Setting: We explored using calibrated thrombin generation (TG) persistent cross-sectional study including viral suppression uninfected controls. Methods: used...
Abstract CCR5 is the main HIV co-receptor. We aimed to (1) compare expression on immune cells between people living with (PLHIV) using combination antiretroviral therapy (cART) and HIV-uninfected controls, (2) relate viral reservoir size (3) assess determinants of expression. This cross-sectional study included 209 PLHIV 323 controls. Percentages CCR5+ (%) mean fluorescence intensity assessed by flow cytometry in monocytes lymphocyte subsets were correlated host factors, HIV-1...
There is ongoing debate as to whether abacavir (ABC) increases the risk for cardiovascular disease(CVD) in people living with HIV (PLHIV) and mechanisms underlying this possible association. We recently showed that use of an ABC-containing regimen was independently associated increased thrombin generation (TG). In present study, we aim explore these findings further, by studying mechanistical processes underly global test via dynamics analysis. Thrombin analysis can pinpoint cause ABC-use...
Efavirenz (EFV) is used for antiretroviral treatment of HIV infection, and successfully inhibits viral replication mother-to-child transmission during pregnancy childbirth. Unfortunately, the drug induces neuropsychiatric symptoms such as anxiety depressed mood potentially affects cognitive performance. EFV acts on, among others, serotonin transporter receptors that are expressed in developing brain. Yet, how perinatal exposure brain cytoarchitecture remains unclear. Here, we exposed...
Systemic chronic inflammation and immune dysfunction are recognized as drivers of the development non-AIDS related comorbidities (NARCs) in people living with HIV (PLHIV). In order to lower risk NARCs, it is critical elucidate what contribution alterations composition function circulating cells NARCs-related pathogenesis. Findings from previous immunophenotyping studies PLHIV highly heterogeneous not fully understood extent phenotypic changes on play a role dysregulated inflammatory response...
Contradictory data have been reported concerning neuropsychiatric side effects of the first-line antiretroviral drug dolutegravir, which may be partly due to lack control groups or psychiatric assessment tools. Using validated self-report questionnaires, we compared mood and anxiety (DASS-42), impulsivity (BIS-11), substance use (MATE-Q) between dolutegravir-treated dolutegravir-naive people living with HIV (PLHIV). We analyzed 194, mostly male, PLHIV on long-term treatment whom 82/194...