A5088196629- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- interferon and immune responses
- Hematopoietic Stem Cell Transplantation
- Immune responses and vaccinations
- Single-cell and spatial transcriptomics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Genetic diversity and population structure
- Gout, Hyperuricemia, Uric Acid
- COVID-19 Clinical Research Studies
- Viral-associated cancers and disorders
- Immune cells in cancer
- Immune Response and Inflammation
- Vitamin D Research Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Forensic Anthropology and Bioarchaeology Studies
- Vaccine Coverage and Hesitancy
- SARS-CoV-2 and COVID-19 Research
- Urticaria and Related Conditions
- Forensic and Genetic Research
- Inflammasome and immune disorders
- COVID-19 Impact on Reproduction
- Hemoglobinopathies and Related Disorders
Radboud University Nijmegen
2020-2024
Radboud University Medical Center
2020-2024
University Medical Center
2023-2024
Radboud Institute for Molecular Life Sciences
2020-2023
Severe coronavirus disease 2019 (COVID-19) can occur in younger, predominantly male, patients without preexisting medical conditions. Some individuals may have primary immunodeficiencies that predispose to severe infections caused by acute respiratory syndrome 2 (SARS-CoV-2).To explore the presence of genetic variants associated with among young COVID-19.Case series pairs brothers history meeting selection criteria (age <35 years) brother admitted intensive care unit (ICU) due COVID-19. Four...
Bacille Calmette-Guérin (BCG) induces long-term boosting of innate immunity, termed trained and decreases susceptibility to respiratory tract infections. BCG vaccination trials for reducing SARS-CoV-2 infection are underway, but concerns have been raised regarding the potential harm strong immune responses. To investigate safety vaccination, we retrospectively assessed coronavirus disease 2019 (COVID-19) related symptoms in three cohorts healthy volunteers who either received last 5 years or...
Gout is characterised by severe interleukin (IL)-1-mediated joint inflammation induced monosodium urate crystals. Since IL-37 a pivotal anti-inflammatory cytokine suppressing the activity of IL-1, we conducted genetic and functional studies aimed at elucidating role in pathogenesis treatment gout.Variant identification was performed DNA sequencing all coding bases IL37 using molecular inversion probe-based resequencing (discovery cohort: gout n=675, controls n=520) TaqMan genotyping...
Haematopoietic clones caused by somatic mutations with ≥2% variant allele frequency (VAF) increase age and are linked to risk of haematological malignancies cardiovascular disease. Recent observations suggest that smaller (VAF<2%) also associated adverse outcomes. Our aims were determine the prevalence clonal haematopoiesis driven variable sizes in individuals obesity treated usual care or bariatric surgery (a treatment improves metabolic status), examine expansion relation dysregulation...
Abstract Clonal hematopoiesis, a common age-related phenomenon marked by expansion of cells with clonal hematopoiesis driver mutations, has been associated all-cause mortality, cancer, and cardiovascular disease. People HIV (PWH) are at risk for non-AIDS–related comorbidities such as atherosclerotic disease cancer. In cross-sectional cohort study, we compared prevalence in PWH on stable antiretroviral therapy overweight individuals age- sex-matched population controls. The adjusted age was...
Few complete human genomes from the European Early Upper Palaeolithic (EUP) have been sequenced. Using novel sampling and DNA extraction approaches, we sequenced genome of a woman "Peştera Muierii," Romania who lived ∼34,000 years ago to 13.5× coverage. The shows similarities modern-day Europeans, but she is not direct ancestor. Although her cranium exhibits both modern Neanderthal features, similar levels admixture (∼3.1%) most EUP humans only half compared ∼40,000-year-old Peştera Oase 1....
Obesity is accompanied by dysregulated inflammation, which can contribute to vasculometabolic complications including metabolic syndrome and atherosclerosis. Recently, clonal hematopoiesis of indeterminate potential (CHIP) has emerged as a risk factor for cardiovascular diseases. We aimed determine how CHIP related immune cell function, systemic in obese individuals. Two hundred ninety-seven individuals with overweight obesity, between the ages 54 81 years, were recruited cross-sectional...
Clonal hematopoiesis (CH) is a risk factor for atherosclerotic cardiovascular disease, but the impact of smaller clones and effect on inflammatory parameters largely unknown. Using ultrasensitive single-molecule molecular inversion probe sequencing, we evaluated association between CH first major adverse event (MACE) in patients with angiographically documented stable coronary artery disease (CAD) no history acute ischemic events. was associated an increased rate MACE at four years...
Abstract Aims Haematopoietic clones caused by somatic mutations with ≥2% variant allele frequency (VAF), known as clonal haematopoiesis of indeterminate potential (CHIP), increase age and have been linked to risk haematological malignancies cardiovascular disease. Recent observations suggest that smaller are also associated adverse clinical outcomes. Our aims were determine the prevalence driven variable sizes, examine development over time in relation metabolic dysregulation up 20 years...
Abstract Background The interleukin (IL)-1 pathway is primarily associated with innate immunological defense and plays a major role in the induction regulation of inflammation. Both common rare genetic variation this underlies various inflammation-mediated diseases, but variants relative to immune response variability healthy individuals remains unclear. Methods We performed molecular inversion probe sequencing on 48 IL-1 pathway-related genes 463 from Human Functional Genomics Project....
Abstract Background Obesity is accompanied by dysregulated inflammation, which can contribute to vasculometabolic complications, including metabolic syndrome and atherosclerosis. Recently, clonal hematopoiesis of indeterminate potential (CHIP) has emerged as a risk factor for cardiovascular diseases. We aimed determine how CHIP related immune cell function, systemic complications in obese individuals. Methods 297 individuals with overweight obesity, between the ages 54 81, were recruited...
ABSTRACT Background Interleukin(IL)-1 signaling is of major importance in human innate cytokine responses. Common variants related genes have been linked to various inflammation-mediated diseases and stimulation-induced responses, but the role rare remains be elucidated. Methods In this study, we characterize common genetic variation, as identified by molecular inversion probe-based sequencing, 48 IL-1 pathway. Using a systems biology approach, examined inter-individual variability vitro...
Introduction: Hematopoietic clones caused by somatic mutations with ≥2% variant allele frequency (VAF) increase age and are linked to risk of hematological malignancies cardiovascular disease. Recent observations suggest that smaller (VAF<2%) also associated adverse outcomes. Individuals obesity have increased disease, it is possible this due prevalence hematopoietic clones. Hypothesis: We assessed the hypothesis obesity-related metabolic dysfunction increases clonal hematopoiesis....
This study aimed to evaluate the incidence, severity and presence of symptoms respiratory tract infections COVID-19, in patients with pre-existing thyroid dysfunction compared individuals without diseases, during peak month COVID-19 pandemic Netherlands.In this retrospective observational cohort study, all currently under follow-up at Radboud UMC for received a digital questionnaire. Primary outcomes were incidence self-reported sickness cases diagnosed COVID-19. We these primary between...