A5010537921- Leptospirosis research and findings
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Salmonella and Campylobacter epidemiology
- Vector-borne infectious diseases
- Malaria Research and Control
- Parasites and Host Interactions
- Pneumonia and Respiratory Infections
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Sepsis Diagnosis and Treatment
- Public Health and Nutrition
- Respiratory viral infections research
- HIV Research and Treatment
- Influenza Virus Research Studies
- Cell death mechanisms and regulation
- Inflammatory Biomarkers in Disease Prognosis
- Cardiac, Anesthesia and Surgical Outcomes
- Digital Imaging for Blood Diseases
- Streptococcal Infections and Treatments
- Animal Virus Infections Studies
- Molecular Biology Techniques and Applications
- COVID-19 Clinical Research Studies
- Diet and metabolism studies
- Platelet Disorders and Treatments
Dr. Kariadi Hospital
2015-2025
Diponegoro University
2013-2024
Universitas 17 Agustus 1945 Semarang
2021-2023
Radboud University Nijmegen
1994
Roche (Switzerland)
1994
ABSTRACT An assay device for the rapid detection of Leptospira -specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) compared well (91.9% agreement) with those an IgM enzyme-linked immunosorbent routinely used serodiagnosis leptospirosis. varied stage disease. uses stabilized components simply performed by addition serum sample fluid to device. read after 10 min, a positive result obtained when staining test line observed.
To identify risk factors for typhoid fever in Semarang city and its surroundings, 75 culture-proven patients discharged 2 weeks earlier from hospital controls were studied. Control subjects neighbours of cases with no history fever, not family members, randomly selected matched gender age. Both interviewed at home by the same trained interviewer using a standardized questionnaire. A structured observation their living environment inside outside house was performed during visit drinking water...
Journal Article Patterns of Proinflammatory Cytokines and Inhibitors during Typhoid Fever Get access Monique Keuter, Keuter Reprints or correspondence: Dr. University Hospital, Dept. Internal Medicine, P.O. Box 9101, 6500 HB Nijmegen, Netherlands. Search for other works by this author on: Oxford Academic PubMed Google Scholar Edi Dharmana, Dharmana M. Hussein Gasem, Gasem Johanna van der Ven-Jongekrijg, Ven-Jongekrijg Robert Djokomoeljanto, Djokomoeljanto Wil V. Dolmans, Dolmans Pierre...
Severe leptospirosis features bleeding and multi-organ failure, leading to shock death. Currently it is assumed that both exaggerated inflammation immune suppression contribute mortality in sepsis. Indeed, several proinflammatory cytokines are reported be induced during leptospirosis. Toll-like receptors, which play an important role the initiation of innate response, inhibited by negative regulators including membrane-bound ST2 (mST2) receptor. Soluble (sST2) has been implicated inhibit...
To determine the involvement of coagulation in bleeding and poor outcome patients with severe leptospirosis.In a prospective study, parameters system were measured on admission during follow-up 52 consecutive leptospirosis.All showed disorders, such as prolonged prothrombin time (PT) activated partial thromboplastin time, marked procoagulant activity [thrombin-antithrombin (TAT) complexes, fragment 1+2, D-dimer], reduced levels anticoagulant markers (protein C, antithrombin) increased...
Abstract Background The burden of leptospirosis in Indonesia is poorly understood. Data from an observational study conducted 2013 to 2016 seven cities across was used estimate the incidence and document its clinical manifestations patients requiring hospitalization. Methods Specimens hospitalized with acute fever were collected at enrollment, 14–28 days, 3 months. Demographic information during visits and/or retrieved medical records double-entered into report forms. After initially...
Introduction Streptococcus pneumoniae is a worldwide occurring pathogen Nasopharyngeal carriage of precedes pneumonia and other pneumococcal diseases in the community. Little known about S. Indonesia, complicating strategies to control diseases. We investigated nasopharyngeal Semarang, Indonesia. Methods A population-based survey was performed swabs questionnaires were taken from 496 healthy young (6–60 month-old) children 45–70 year-old adults. Results Forty-three percent aged 6–60 months...
Summary Platelets and platelet-monocyte interaction play an important role in inflammation. Both pro- anti-inflammatory effects of platelet inhibition have been reported animal models. This study aimed to investigate the effect platelets by new P2Y12 receptor antagonist ticagrelor on monocyte function, as assessed cytokine responses Toll-like Receptor (TLR) ligands. In a set vitro experiments, peripheral blood mononuclear cells (PBMC) incubated with TLR2 ligand Pam3CSK4 produced less...
Thrombocytopenia and platelet dysfunction are commonly observed in patients with dengue virus (DENV) infection may contribute to complications such as bleeding plasma leakage. The etiology of dengue-associated thrombocytopenia is multifactorial includes increased clearance. binding the coagulation protein von Willebrand factor (VWF) membrane removal sialic acid (desialylation) two well-known mechanisms clearance, but whether these conditions also unknown. In observational cohort studies...
Clostridium difficile has not been studied in detail Asia, particularly Southeast Asia. We thus performed a prevalence study across four hospitals Central Java province, Indonesia. Stool samples were collected from patients with diarrhoea and tested by enzyme immunoassay for glutamate dehydrogenase (GDH) toxin A/B (C DIFF QUIK CHEK COMPLETE, TechLab). Specimens cultured molecular typing was performed. In total, 340 tested, of which 70 (20.6%) GDH positive, detected 19 (5.6%). Toxigenic C....
Background The epidemiology of acute febrile illness, a common cause hospitalization in Indonesia, has not been systematically studied. Methodology/Principal findings This prospective observational study enrolled patients (temperature ≥38°C) aged ≥1 year from July 2013 until June 2016 at eight government referral teaching hospitals seven provincial capitals Indonesia. Patients were managed according to the hospital standard-of-care (SOC), and blood samples drawn for molecular serological...
To investigate rickettsioses and leptospirosis among urban residents of Semarang, Indonesia, we tested the blood 137 patients with fever. Evidence Rickettsia typhi, agent murine typhus, was found in 9 patients. Another showed inconclusive serologic results. Thirteen received a diagnosis leptospirosis. No dual infections were detected.
Gram-negative bacilli (GNB) cause many cases of pneumonia in Indonesia. We investigated nasopharyngeal carriage GNB Semarang, Klebsiella pneumoniae adults (15%) was higher than children (7%) (P = 0.004), while that other comparable. Poor food and water hygiene are determinants these bacteria.
ABSTRACT Leptospirosis is an endemic zoonotic disease with protean clinical manifestations caused by pathogenic spirochetes of the Leptospira genus. The microscopic agglutination test (MAT) gold standard for leptospirosis diagnosis and can only be conducted in a reference laboratory. Therefore, alternative tests, such as IgM anti- rapid diagnostic (RDT), are preferred general use. In this study, we aimed to compare accuracy four products detection RDTs, which available Indonesia, against...
Leptospirosis is an often severe disease which requires prompt treatment. Laboratory testing required to reach a valid diagnosis. An agglutination assay for the detection of Leptospira‐specific antibodies consisting individually wrapped cards containing stable, dried reagent evaluated. The simply performed by suspending with drop serum. result obtained within 30 s. sensitivity varied stage and was 72.3% samples collected during first 10 days illness 88.2% at later stage. specificity 93.9%...
Chikungunya virus (CHIKV) is often overlooked as an etiology of fever in tropical and sub-tropical regions. Lack diagnostic testing capacity these areas combined with co-circulation clinically similar pathogens such dengue (DENV), hinders CHIKV diagnosis. To better address Indonesia, improved understanding epidemiology, clinical presentation, approaches needed.
ABSTRACT We performed a randomized controlled trial involving 55 adult patients with enteric fever to compare ciprofloxacin and chloramphenicol. Blood bone marrow cultures cytokine profiles during therapy were done the clinical bacteriological efficacies of these drugs. All randomly assigned receive chloramphenicol (500 mg four times day orally) for 14 days or twice 7 days. In each treatment group, subsequently have blood cultured after either 3 5 treatment. Twenty-seven received...
To support the clinical diagnosis of typhoid fever in Indonesia, where most hospitals and health centres have no facilities for culture, a rapid dipstick assay detection Salmonella typhi-specific IgM antibodies was evaluated on serum samples from 127 patients clinically suspected having fever. In single blood sample collected admission to hospital, sensitivity 69.8% when compared with bone marrow culture 86.5% culture. The specificity as calculated group but negative result be 88.9%. Of 80...
Background Severe leptospirosis is frequently complicated by a hemorrhagic diathesis, of which the pathogenesis still largely unknown. Thrombocytopenia common, but often not to degree that spontaneous bleeding expected. We hypothesized complications are only related thrombocytopenia, also platelet dysfunction, and increased binding von Willebrand factor (VWF) platelets involved in both dysfunction clearance. Methodology/Principal findings A prospective study was carried out Semarang,...
The outcome in three groups of patients with bacteriologically confirmed typhoid fever caused by Salmonella typhi, treated during episodes between 1948 and 1990 Java, Indonesia, was compared retrospective analysis hospital records. study population consisted patients. Group I ( n = 50) Batavia (the present Jakarta) from to 1950, II 61) Yogyakarta 1952 1956, III 105) Semarang 1989 1990. Main measures were days until defervescence, early relapses hospitalization, duration stay, complications...