A5039851428- Leptospirosis research and findings
- Viral Infections and Vectors
- Veterinary medicine and infectious diseases
- Zoonotic diseases and public health
- Humic Substances and Bio-Organic Studies
- Viral Infections and Outbreaks Research
- Vector-borne infectious diseases
- Biomarkers in Disease Mechanisms
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Yersinia bacterium, plague, ectoparasites research
- Research on Leishmaniasis Studies
- Bacillus and Francisella bacterial research
- Mycobacterium research and diagnosis
- Fire effects on ecosystems
- Galectins and Cancer Biology
- Toxoplasma gondii Research Studies
- Blood groups and transfusion
- Animal health and immunology
- Vector-Borne Animal Diseases
- Intensive Care Unit Cognitive Disorders
- Neonatal Health and Biochemistry
- Bartonella species infections research
- Animal Virus Infections Studies
- Tryptophan and brain disorders
University Medical Center Groningen
2024
University of Groningen
2024
University of Amsterdam
2018-2023
Amsterdam University Medical Centers
2019-2023
Amsterdam UMC Location University of Amsterdam
2018-2021
Laboklin (Germany)
2019
Royal Tropical Institute
2008-2018
Institut de Neurobiologie de la Méditerranée
2009
Regional Medical Research Centre
2003
Indian Council of Medical Research
2003
Background Leptospirosis, a spirochaetal zoonosis, occurs in diverse epidemiological settings and affects vulnerable populations, such as rural subsistence farmers urban slum dwellers. Although leptospirosis can cause life-threatening disease, there is no global burden of disease estimate terms Disability Adjusted Life Years (DALYs) available. Methodology/Principal Findings We utilised the results parallel publication that reported estimates morbidity mortality due to leptospirosis....
Abstract The microscopic agglutination test (MAT) is the gold standard for sero‐diagnosis of leptospirosis because its unsurpassed diagnostic specificity. It uses panels live leptospires, ideally recent isolates, representing circulating serovars from area where patient became infected. A dilution series patient's serum mixed with a suspension leptospires in microtiter plates. After incubating about 2 hr at 30°C, results are read under dark‐field microscope. titer last which ≥50% have...
Leptospirosis is a zoonotic bacterial disease that affects more than one million people worldwide each year. Human infection acquired through direct or indirect contact with the urine of an infected animal. A wide range animals including rodents and livestock may shed Leptospira bacteria act as source for people. In Kilimanjaro Region northern Tanzania, leptospirosis important cause acute febrile illness, yet relatively little known about animal hosts in this area. The roles ruminant...
We studied a dipstick assay for the detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human serum samples. A high degree concordance was observed between results and an IgM enzyme-linked immunosorbent (ELISA). Application acute leptospirosis enabled accurate identification, early disease, proportion cases leptospirosis. Analysis second sample is recommended, order to determine seroconversion or increased staining intensity. All samples from patients who were confirmed be...
Background Diagnosis of leptospirosis by the microscopic agglutination test (MAT) or culture is confined to specialized laboratories. Although ELISA techniques are more common, they still require laboratory facilities. Rapid Diagnostic Tests (RDTs) can be used for easy point-of-care diagnosis. This study aims evaluate diagnostic performance RDTs LeptoTek Dri Dot, Lateral Flow, and Leptocheck-WB, prospectively. Methodology During 2001 2012, one two at same time have been applied prior routine...
To increase knowledge of leptospirosis in the Netherlands and identify changing trends this disease over time, we analyzed historical passive surveillance reports for an 84-year period (1925-2008). We found that 2,553 mainly severe cases were diagnosed (average annual incidence rate 0.25 cases/100,000 population). The overall case-fatality patients with reported was 6.5% but decreased period, probably because improved treatment. Ninety percent male patients. Most autochthonous infections...
Background Leptospirosis, commonly known as rat-urine disease, is a global but endemic zoonotic disease in the tropics. Despite historical report of leptospirosis Malaysia, information on human-infecting species limited. Determining circulating important to understand its epidemiology, thereby strategize appropriate control measures through public health interventions, diagnostics, therapeutics and vaccine development. Methodology/Principle findings We investigated Leptospira blood serum...
Two spirochetes (designated strains LGVF01 and LGVF02 T ) were isolated from soil samples in San Juan, Puerto Rico HAN media after selection using a combination of ELISA, agar plating, colony screening by Fluorescent Antibody Testing (FAT) PCR for lipL32 secY . Isolates helix-shaped, aerobic, fast-growing, highly motile. Genome sequence analysis indicated that both should be classified as members novel species within the pathogenic (P1) clade genus Leptospira The average nucleotide identity...
In order to increase the application potential of direct agglutination test (DAT) for detection anti-Leishmania antibodies in human serum samples, we developed an antigen based on stained and freeze-dried Leishmania donovani promastigotes. We describe here evaluation performance DAT this antigen. It was shown that remains fully active, even after storage at 56 degrees C 18 months. With a cutoff value 1:1,600, sensitivity be 92% specificity 99.7%, which were comparable with results found...
ABSTRACT A newly developed latex agglutination assay for the detection of genus-specific Leptospira antibodies in human sera was evaluated. The is performed by mixing, on an card, serum with equal volumes stabilized antigen-coated, dyed test and control beads read within 2 min. evaluated groups samples from patients leptospirosis Hawaii, Seychelles, Thailand, Netherlands. mean overall sensitivity 82.3%, specificity 94.6%. easy to perform does not require special skills or equipment. reagents...
Severe leptospirosis features bleeding and multi-organ failure, leading to shock death. Currently it is assumed that both exaggerated inflammation immune suppression contribute mortality in sepsis. Indeed, several proinflammatory cytokines are reported be induced during leptospirosis. Toll-like receptors, which play an important role the initiation of innate response, inhibited by negative regulators including membrane-bound ST2 (mST2) receptor. Soluble (sST2) has been implicated inhibit...
To determine the involvement of coagulation in bleeding and poor outcome patients with severe leptospirosis.In a prospective study, parameters system were measured on admission during follow-up 52 consecutive leptospirosis.All showed disorders, such as prolonged prothrombin time (PT) activated partial thromboplastin time, marked procoagulant activity [thrombin-antithrombin (TAT) complexes, fragment 1+2, D-dimer], reduced levels anticoagulant markers (protein C, antithrombin) increased...
BackgroundLeptospirosis is a global zoonotic disease. Although important for the assessment of burden leptospirosis, data on duration illness and occurrence post-leptospirosis complaints are not well documented. Hence main objective this study was to estimate persistent hospital stay in laboratory confirmed leptospirosis patients Netherlands during 1985 2010. Additionally, several risk factors potentially impacting were investigated. Methods/Principal FindingsThe acute phase 16 days (IQR...
In the Netherlands, 97 human leptospirosis cases were notified in 2014. This represents a 4.6-fold increase autochthonous (n = 60) compared with annual average between 2010 and 2013. Most had symptom onset June November. marked humans coincided an of dogs. 2014, 13 dogs reported, two to six annually from The majority 20) linked recreational exposure, e.g. swimming or fishing, followed by occupational exposure 15). About sixty per cent 37) most likely attributable surface water contact,...
ABSTRACT We performed a multicenter evaluation of robust and easily dipstick assay for the serodiagnosis human leptospirosis. The is aimed at detection Leptospira -specific immunoglobulin M (IgM) antibodies. study involved 2,665 serum samples collected from 2,057 patients with suspected leptospirosis in 12 countries on five continents different levels endemicity surveillance systems. were grouped as laboratory-confirmed case noncase based results culturing microscopic agglutination test....
Summary The pathogenesis of equine recurrent uveitis (ERU) is not completely understood. Persistent infection pathogenic leptospires in the eyes probable primary cause. Consistently, we isolated and characterized this study from 32.2% intraocular samples collected 501 horses following Western European countries: Germany, Switzerland, Austria, Netherlands, Luxembourg, Great Britain, Italy Poland. vast majority leptospiral isolates belonged to serogroup Grippotyphosa (78.2%). other Australis...
ABSTRACT A proficiency testing scheme for the leptospirosis microscopic agglutination test was provided to 37 laboratories in 23 countries 2002 (round 1) and 60 34 2003 2). Thirty-four participated both rounds. Each panel consisted of five rabbit serum samples, four which were antisera raised against pathogenic serovars Leptospira . One these samples a mixture two different antisera. The rates false-negative results, calculated on basis assumption that within serogroup will cross-react, 11%...
Leptospirosis is caused by pathogenic spirochetes of the genus Leptospira. The bacteria enter human body via abraded skin or mucous membranes and may disseminate throughout. In general clinical picture mild but some patients develop rapidly progressive, severe disease with a high case fatality rate. Not much known about innate immune response to leptospires during haematogenous dissemination. Previous work showed that THP-1 cell line recognized heat-killed leptospiral LPS through TLR2...
Leptospirosis is a globally emerging zoonotic disease, associated with various climatic, biotic and abiotic factors. Mapping quantifying geographical variations in the occurrence of leptospirosis surrounding environment offer innovative methods to study disease transmission identify associations between environment. This aims investigate geographic incidence Netherlands environmental factors driving emergence disease. Individual case data derived over period 1995-2012 were geocoded...
Abstract Background Leptospirosis is a widespread zoonosis and has been recognized as re-emerging infectious disease in humans dogs, but prevalence of Leptospira shedding dogs Thailand unknown. The aim this study was to determine urinary Thailand, evaluate antibody by microscopic agglutination test (MAT) enzyme-linked immunosorbent assay (ELISA), assess risk factors for infection. In Northern, Northeastern, Central 273 stray ( n = 119) or client-owned 154) from rural 139) urban 134) areas...
To investigate rickettsioses and leptospirosis among urban residents of Semarang, Indonesia, we tested the blood 137 patients with fever. Evidence Rickettsia typhi, agent murine typhus, was found in 9 patients. Another showed inconclusive serologic results. Thirteen received a diagnosis leptospirosis. No dual infections were detected.