A5033901438- Salmonella and Campylobacter epidemiology
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Bacterial Identification and Susceptibility Testing
- Microbial infections and disease research
- Bacterial biofilms and quorum sensing
- Clostridium difficile and Clostridium perfringens research
- Animal Nutrition and Physiology
- Aquaculture disease management and microbiota
- Mycobacterium research and diagnosis
- Antibiotic Use and Resistance
- Genomics and Phylogenetic Studies
- Food Safety and Hygiene
- Veterinary medicine and infectious diseases
- Gut microbiota and health
- Animal Behavior and Welfare Studies
- Parasitic Infections and Diagnostics
- Animal Disease Management and Epidemiology
- Streptococcal Infections and Treatments
- Leptospirosis research and findings
- Listeria monocytogenes in Food Safety
- Escherichia coli research studies
Utrecht University
2016-2025
Wageningen University & Research
2016-2025
Biology of Infection
2019-2022
Ministry of Agriculture, Nature and Food Quality
2021
Netherlands Food and Consumer Product Safety Authority
2021
Quadram Institute
2020
Colombian Corporation for Agricultural Research - AGROSAVIA
2020
Instituto Costarricense de Investigación y Enseñanza en Nutrición y Salud
2020
Universidad del Valle de Guatemala
2020
Institut Pasteur
2020
In this paper we describe a number of immunological parameters for dogs with chronic Leishmania infantum infection which exhibit patterns progressive disease or apparent resistance. The outcome was assessed by isolation parasites, serum antibody titers to antigen, and development clinical signs leishmaniasis. Our studies show that 3 years after experimental infection, asymptomatic resistant responded L. antigen both in lymphocyte proliferation assays vitro delayed-type hypersensitivity...
The aim of this study was to determine the phenotypic and genotypic resistance profiles methicillin-resistant Staphylococcus pseudintermedius (MRSP) examine clonal distribution in Europe North America. A total 103 MRSP isolates from dogs isolated several countries Europe, USA Canada were characterized. Isolates identified by PCR–restriction fragment length polymorphism (RFLP), antimicrobial susceptibility determined broth dilution or gradient diffusion, genes detected using a microarray....
Abstract We isolated methicillin-resistant Staphylococcus aureus (MRSA) from cows with subclinical mastitis and a person who worked these animals. The bovine human strains were indistinguishable by phenotyping genotyping methods of low frequency spa type. To our knowledge, this finding indicates the first documented case direct transmission MRSA between humans.
Acute enteric infections caused by salmonellas remain a major public health burden worldwide. Poultry, particularly chickens, are known to be the main reservoir for this zoonotic pathogen. Although some progress has been made in reducing Salmonella colonization of broiler chickens using biosecurity and antimicrobials, it still remains considerable problem. The use host-specific bacteriophages as biocontrol is one possible intervention which could reduced. A total 232 were isolated from...
Recently a specific MRSA sequence type, ST398, emerged in food production animals and farmers. Risk factors for carrying ST398 both humans have not been fully evaluated. In this cross-sectional study, we investigated associated with colonization veal calves working living on these farms.A sample of 102 calf farms were randomly selected visited from March 2007-February 2008. Participating farmers asked to fill questionnaire (n = 390) identify potential risk factors. A nasal swab was taken...
Summary Use of antimicrobials in animals poses a potential risk for public health as it contributes to the selection and spread antimicrobial resistance. Although knowledge negative consequences extensive use humans accumulated over decades, total therapeutic farm Netherlands doubled between 1990 2007. A series facts events formed window opportunity reduce animals. The recent discovery significant reservoirs antimicrobial‐resistant pathogens such methicillin‐resistant Staphylococcus aureus (...
To investigate the occurrence and characteristics of extended-spectrum β-lactamase (ESBL)- AmpC-producing Enterobacteriaceae isolates in clinical samples companion animals horses compare results with ESBL/AmpC-producing described humans. Between October 2007 August 2009, 2700 derived from infections were collected. Isolates displaying inhibition zones ≤25 mm for ceftiofur and/or cefquinome by disc diffusion included. ESBL/AmpC production was confirmed combination tests. The presence...
Extended-spectrum-β-lactamase (ESBL)/AmpC producing Enterobacteriaceae have been reported worldwide amongst isolates obtained from humans, food-producing animals, companion and environmental sources. However, data on prevalence of fecal carriage ESBL/AmpC in healthy animals is limited. This pilot study describes the encoding genes cats dogs, dogs with diarrhea. Twenty samples each group were cultured MacConkey agar supplemented 1 mg/L cefotaxime LB-enrichment broth cefotaxime, which was...
The close contact between household pets and people offers favourable conditions for bacterial transmission. In this article, the aetiology, prevalence, transmission, impact on human health preventative measures are summarized selected zoonoses transmissible by pets. Six representing distinct transmission routes were arbitrarily based available information incidence severity of pet-associated disease caused zoonotic bacteria: bite infections cat scratch (physical injuries), psittacosis...
In recent years, ESBL/AmpC-producing Escherichia coli (ESBL/AmpC-EC) have been isolated with increasing frequency from animals, food, environmental sources and humans. With incomplete scattered evidence, the contribution to human carriage burden these reservoirs remains unclear.To quantify molecular similarities between different as a first step towards risk attribution.Pooled data on ESBL/AmpC-EC isolates were recovered 35 studies in Netherlands comprising >27 000 samples, mostly obtained...
Enterococci are gut microbes of most land animals. Likely appearing first in the guts arthropods as they moved onto land, diversified over hundreds millions years adapting to evolving hosts and host diets. Over 60 enterococcal species now known. Two species,
A total of 1,794 migrating birds trapped at a coastal site in southern Sweden were sampled for detection Campylobacter spp. All isolates phenotypically identified as jejuni and subset those non-C. to the species level by PCR-based techniques. C. was found 5.0% birds, lari 5.6%, coli 0.9%. An additional 10.7% tested infected with hippurate hydrolysis-negative that not level. The prevalence differed significantly between ecological guilds birds. Shoreline-foraging feeding on invertebrates...
Abstract A total of 814 isolates the foodborne pathogen Campylobacter jejuni were characterized by multilocus sequence typing (MLST) and analysis variation two cell-surface components: heat-stable (HS) serotyping antigen flagella protein FlaA short variable region (SVR). We identified 379 combinations MLST loci (sequence types) 215 components among these isolates, which had been obtained from human disease, animals, food, environment. Despite this diversity, 748 (92%) belonged to one 17...
ABSTRACT Three molecular typing methods were used to study the relationships among 184 Campylobacter strains isolated from humans, cattle, and chickens. All genotyped by amplified fragment length polymorphism (AFLP) analysis, multilocus sequence (MLST), analysis of a genomic region with short tandem repeats designated clustered regularly interspaced palindromic (CRISPRs). MLST AFLP yielded more than 100 different profiles patterns, respectively. These multiple-locus resulted in similar...
Introduction The presence of Livestock Associated MRSA (LA-MRSA) in humans is associated with intensity animal contact. It unknown whether the LA-MRSA a result carriage or retention MRSA-contaminated dust. We conducted longitudinal study among 155 veal farmers which repeated nasal and throat swabs were taken for detection. Periods without exposure covered. Methods Randomly, 51 calf farms visited from June - December 2008. Participants asked to fill questionnaires (n = 155) identify potential...
Campylobacteriosis contributes strongly to the disease burden of food-borne pathogens. Case-control studies are limited in attributing human infections different reservoirs because they can only trace back points exposure, which may not point original cross-contamination. Human Campylobacter be attributed specific by estimating extent subtype sharing between strains from humans and using multilocus sequence typing (MLST).We investigated risk factors for campylobacteriosis caused reservoirs....
As a major foodborne pathogen, Campylobacter jejuni receives much attention in quantitative risk assessment. To date, all dose-response assessments have been based on single human feeding study which unfortunately provides incomplete and possibly biased information the relation. An incident at dairy farm, where several children from school class became ill as result of drinking raw milk contaminated with C. jejuni, appeared to show very clear relation between amount consumed attack rate....