A5040604703- Antimicrobial Resistance in Staphylococcus
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial Identification and Susceptibility Testing
- Microbial infections and disease research
- Mycobacterium research and diagnosis
- Milk Quality and Mastitis in Dairy Cows
- Bacteriophages and microbial interactions
- Microbial Metabolism and Applications
- Brucella: diagnosis, epidemiology, treatment
- Animal Nutrition and Physiology
- Aquaculture disease management and microbiota
- Gut microbiota and health
- Escherichia coli research studies
- Bird parasitology and diseases
- Clostridium difficile and Clostridium perfringens research
- Listeria monocytogenes in Food Safety
- Streptococcal Infections and Treatments
- Trace Elements in Health
- Galectins and Cancer Biology
- Veterinary medicine and infectious diseases
- Antibiotic Use and Resistance
- Identification and Quantification in Food
Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana
2016-2025
Technical University of Denmark
2011
Istituto Superiore di Sanità
1997-2005
Sapienza University of Rome
1987
Antimicrobial resistance (AMR) is a serious threat to global public health, but obtaining representative data on AMR for healthy human populations difficult. Here, we use metagenomic analysis of untreated sewage characterize the bacterial resistome from 79 sites in 60 countries. We find systematic differences abundance and diversity genes between Europe/North-America/Oceania Africa/Asia/South-America. taxonomy only explains minor part variation that observe. no evidence cross-selection...
Since its discovery in the early 2000s, methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 398 (CC398) has become a rapidly emerging cause of human infections, most often associated with livestock exposure. We applied whole-genome sequence typing to characterize diverse collection CC398 isolates (n = 89), including MRSA and methicillin-susceptible S. (MSSA) from animals humans spanning 19 countries four continents. identified 4,238 single nucleotide polymorphisms (SNPs) among...
Background and aimPlasmid-mediated colistin resistance mechanisms have been identified worldwide in the past years. A multiplex polymerase chain reaction (PCR) protocol for detection of all currently known transferable genes (mcr-1 to mcr-5, variants) Enterobacteriaceae was developed surveillance or research purposes. Methods: We designed four new primer pairs amplify mcr-1, mcr-2, mcr-3 mcr-4 gene products used originally described primers mcr-5 obtain a stepwise separation ca 200 bp...
The aim of this study was to determine the phenotypic and genotypic resistance profiles methicillin-resistant Staphylococcus pseudintermedius (MRSP) examine clonal distribution in Europe North America. A total 103 MRSP isolates from dogs isolated several countries Europe, USA Canada were characterized. Isolates identified by PCR–restriction fragment length polymorphism (RFLP), antimicrobial susceptibility determined broth dilution or gradient diffusion, genes detected using a microarray....
Proposals to update the harmonised monitoring and reporting of antimicrobial resistance (AMR) from a public health perspective in
Abstract During the late summer of 1998, veterinary authorities in Tuscany, Italy, received reports cases neurologic disease among horses residing a large wetland area located provinces Florence and Pistoia. West Nile virus was isolated from two six that died or were euthanized. A retrospective epidemiologic study identified 14 clinical occurred August 20 to October 6 (attack rate 2.8%). serologic survey conducted over 700-km2 stables with without apparent confirmed wider spread infection,...
We report the first diagnostic test for identification of Staphylococcus pseudintermedius involving a simple PCR-restriction fragment length polymorphism approach. The method allows discrimination S. from closely related members intermedius group and other important staphylococcal pathogens humans dogs.
This study was initiated to collect retrospective information on the occurrence of plasmid-mediated quinolone resistance (PMQR) in Salmonella enterica and Escherichia coli isolates Europe identify responsible genes.Databases national reference laboratories containing MIC values for E. isolated between 1994 2009 animals, humans, food environment from 13 European countries were screened exhibiting a defined phenotype, i.e. reduced susceptibility fluoroquinolones nalidixic acid. PCR sequence...
We report the spread of a clone multidrug-resistant (MDR), ESBL-producing (blaCTX-M-1) Salmonella enterica subsp. serovar Infantis, in Italian broiler chicken industry and along food-chain. This was first detected Italy 2011 led to human infection 2013–2014.A set (n = 49) extended-spectrum cephalosporin (ESC)-resistant (R) isolates S. Infantis (2011–2014) from humans, food-producing animals meat thereof, were studied with selected earlier more recent ESC-susceptible (ESC-S) 42, 2001–2014)....
A total of 227 Staphylococcus aureus colonies, isolated from 54 samples raw milk and dairy products bovine, ovine, caprine bubaline origin were tested for the presence genes coding staphylococcal enterotoxins (SEs/SEls) methicillin resistance. Ninety-three 31 (57.4%) 18 (69.2%) 26 farms positive SEs/SEls genes. Most isolates harboured more than one toxin gene 15 different toxinotypes recorded. The most frequent "sec" alone (28.6%), "sea, sed, ser, selj" (20%), "seg, sei" "seh" (8.6%). 77...
Companion animals have been described as potential reservoirs of antimicrobial resistance (AMR), however data remain scarce. Therefore, the objectives were to describe usage (AMU) in dogs and cats three European countries (Belgium, Italy, The Netherlands) investigate phenotypic AMR. A questionnaire one fecal sample per animal (n = 303) collected over year AMU was quantified using treatment incidence (TI). Phenotypic profiles 282 Escherichia coli isolates determined. Nineteen percent received...
Methicillin-resistant Staphylococcus aureus (MRSA) Sequence Type (ST)1, Clonal Complex(CC)1, SCCmec V is one of the major Livestock-Associated (LA-) lineages in pig farming industry Italy and associated with pigs other European countries. Recently, it has been increasingly detected Italian dairy cattle herds. The aim this study was to analyse differences between ST1 MRSA methicillin-susceptible S. (MSSA) from herds Europe human isolates. Sixty-tree animal isolates different holdings 20 were...
Salmonella Infantis is one of the five serovars most frequently causing human salmonellosis in Europe, mainly associated with poultry. A clone harbouring a conjugative plasmid emerging S. (pESI)-like megaplasmid, carrying multidrug resistant (MDR) and extended-spectrum beta-lactamases (ESBL) genes, has spread Italian broiler chicken industry also illness. This work aimed at elucidating molecular epidemiology pESI-like Europe using whole-genome sequencing bioinformatics analysis, to...
We investigated the evolution and epidemiology of a novel livestock-associated methicillin-resistant Staphylococcus aureus strain, which colonizes infects urban-dwelling Danes even without Danish animal reservoir. Genetic evidence suggests both poultry human adaptation, with meat implicated as probable source.
Colistin resistance by mobilisable mcr genes has been described in bacteria of food-animal origin worldwide, which raised public health concerns about its potential foodborne transmission to human pathogenic bacteria. Here we provide baseline information on the molecular epidemiology colistin-resistant, mcr-positive E. coli and Salmonella isolates food-producing animals Italy 2014-2015. A total 678, 861 236 indicator coli, Extended Spectrum Beta-Lactamase (ESBL)/AmpC-producing isolates,...
Abstract The emergence of antimicrobial resistance (AMR) is one the biggest health threats globally. In addition, use drugs in humans and livestock considered an important driver resistance. commensal microbiota, especially intestinal has been shown to have role AMR. Mobile genetic elements (MGEs) also play a central facilitating acquisition spread AMR genes. We isolated Escherichia coli (n = 627) from fecal samples respectively 25 poultry, 28 swine, 15 veal calf herds 6 European countries...
ABSTRACT We report expanded-spectrum cephalosporin resistance in Escherichia coli from dogs and cats Rome, Italy. Three major β-lactamases (CMY-2, SHV-12, CTX-M-1) are reported for the first time E. sick healthy cats. Molecular characterization suggests presence of several combinations β-lactamase genes companion animals.
We report the genetic characterization of 15 Klebsiella pneumoniae (KP) and 4 isolates K. oxytoca (KO) from clinical cases in dogs cats showing extended-spectrum cephalosporin (ESC) resistance. Extended spectrum beta-lactamase (ESBL) AmpC genes, plasmid-mediated quinolone resistance (PMQR) co-resistances were investigated. Among KP isolates, ST101 clone was predominant (8/15, 53%), followed by ST15 (4/15, 27%). ST11 ST340, belonging to Clonal Complex (CC)11, detected 2012 (3/15, 20%). MLST...
ABSTRACT Pandemic methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 97 (CC97) lineages originated from livestock-to-human host jumps. In recent years, CC97 has become one of the major MRSA detected in Italian farmed animals. The aim this study was to characterize and analyze differences methicillin-susceptible S. (MSSA) mainly swine bovine origins. Forty-seven isolates, 35 6 MSSA isolates different pig cattle holdings; 5 Germany; 1 human isolate Spain were characterized by...
ObjectivesThe aim of this study was to provide molecular characterization methicillin-resistant Staphylococcus aureus (MRSA) spa type t127, sequence (ST) 1 isolates, detected in a European baseline survey holdings breeding pigs, determine phenotypic and genotypic drug resistance compare the results with those obtained from collection ST1 MRSA methicillin-susceptible S. (MSSA) clinical isolates.
To compare methicillin-resistant Staphylococcus pseudintermedius (MRSP) and methicillin-susceptible S (MSSP) infections in dogs.Multicenter case-control study.Dogs with MRSP were matched, by hospital, 2 MSSP controls, the occurring immediately before after case infection.Signalment, historical, clinical, treatment, outcome data documented. Conditional logistic regression was performed. A manual stepwise backward elimination procedure used to build multivariable model.56 112 control dogs...
Previous studies in food-producing animals have shown associations between antimicrobial use (AMU) and resistance (AMR) specifically isolated bacterial species. Multi-country data are scarce only describe between-country differences. Here we investigate the pig faecal mobile resistome characteristics at farm-level across Europe.A cross-sectional study was conducted among 176 conventional farms from nine European countries. Twenty-five samples fattening pigs were pooled per farm acquired...