A5007638155- Legume Nitrogen Fixing Symbiosis
- Pharmaceutical and Antibiotic Environmental Impacts
- Plant nutrient uptake and metabolism
- Fecal contamination and water quality
- Microbial Community Ecology and Physiology
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Coastal wetland ecosystem dynamics
- Plant-Microbe Interactions and Immunity
- Water Treatment and Disinfection
- Gut microbiota and health
- Groundwater flow and contamination studies
- Probiotics and Fermented Foods
- Pesticide and Herbicide Environmental Studies
- Wastewater Treatment and Nitrogen Removal
- Plant Pathogens and Fungal Diseases
- Nematode management and characterization studies
- Mycobacterium research and diagnosis
- Environmental Justice and Health Disparities
- Environmental DNA in Biodiversity Studies
- Agronomic Practices and Intercropping Systems
- Salmonella and Campylobacter epidemiology
- Meat and Animal Product Quality
- Plant Pathogenic Bacteria Studies
University of Regina
2015-2024
University of Manitoba
2022
Florida College
2021
University of Florida
2021
Deleted Institution
2017
University of Calgary
1995-2004
Agriculture and Agri-Food Canada
2000-2003
University of California, San Francisco
2003
Antimicrobial resistance (AMR) is a serious threat to global public health, but obtaining representative data on AMR for healthy human populations difficult. Here, we use metagenomic analysis of untreated sewage characterize the bacterial resistome from 79 sites in 60 countries. We find systematic differences abundance and diversity genes between Europe/North-America/Oceania Africa/Asia/South-America. taxonomy only explains minor part variation that observe. no evidence cross-selection...
Abstract Antimicrobial resistance (AMR) is a major threat to global health. Understanding the emergence, evolution, and transmission of individual antibiotic genes (ARGs) essential develop sustainable strategies combatting this threat. Here, we use metagenomic sequencing analyse ARGs in 757 sewage samples from 243 cities 101 countries, collected 2016 2019. We find regional patterns resistomes, these differ between subsets corresponding drug classes are partly driven by taxonomic variation....
Early seedling root growth of the nonlegumes canola (Brassica campestris cv. Tobin, Brassica napus Westar) and lettuce (Lactuca saliva Grand Rapids) was significantly promoted by inoculation seeds with certain strains Rhizobium leguminosarum, including nitrogen- nonnitrogen-fixing derivatives under gnotobiotic conditions. The growfh-promotive effect appears to be direct, possible involvement plant regulators indole-3-acetic acid cytokinin. Auxotrophic mutants requiring tryptophan or...
Rhizobium leguminosarum biovar viciae strain 3841 is a motile alpha-proteobacterium that can establish nitrogen-fixing symbiosis within the roots of pea plants. In order to determine contribution chemotaxis lifestyle R. leguminosarum, we have characterized function two gene clusters (che1 and che2) in controlling motility behaviour. We found both modulate swimming bias cells che1 cluster major pathway chemotaxis. The che2 also contributes bias, but has minor effect on Using competitive...
Rhizobium leguminosarum is a soil bacterium with the ability to form nitrogen-fixing nodules on roots of leguminous plants. Soil-dwelling, free-living R. often encounters desiccation stress, which impacts its survival within soil. The mechanisms by bacteria resist effects stress have been described. However, role cell envelope in tolerance rhizobia relatively uncharacterized. Using transposon mutagenesis approach, mutant bv. viciae was isolated that highly sensitive desiccation. mutation...
Abstract The rapid development of megacities, and their growing connectedness across the world is becoming a distinct driver for emerging disease outbreaks. Early detection unusual emergence spread should therefore include such cities as part risk-based surveillance. A catch-all metagenomic sequencing approach urban sewage could potentially provide an unbiased insight into dynamics viral pathogens circulating in community irrespective access to care, potential which already has been proven...
ABSTRACT The possible origin of beef contamination and genetic diversity Escherichia coli populations in cattle, on carcasses ground beef, was examined by using random amplification polymorphic DNA (RAPD) PCR-restriction fragment length polymorphism (PCR-RFLP) analysis the fliC gene. E. recovered from feces 10 cattle during pasture grazing feedlot finishing hides, carcasses, after slaughter. 1,403 isolates (855 fecal, 320 hide, 153 carcass, 75 beef) were grouped into 121 subtypes RAPD...
The lipopolysaccharide (LPS) of the Gram-negative legume symbiont Rhizobium leguminosarum biovar viciae 3,841 contains several unique modifications, including addition a 27-hydroxyoctacosanoic acid (27OHC28 : 0), also termed very long chain fatty (VLCFA), attached at 2' position lipid A. A transposon mutant that lacks expression two putative 3-oxo-acyl [acyl-carrier protein] synthase II genes, fabF1 and fabF2, from VLCFA biosynthetic cluster, was isolated characterized. MS indicated lacked...
Foods and related processing environments are commonly contaminated with the pathogenic Listeria monocytogenes. To investigate potential environmental reservoirs of spp. L. monocytogenes, surface water point source pollution samples from an urban a rural municipal supply watershed in Nova Scotia, Canada, were examined over 18 months. Presumptive cultured 72 35% samples, respectively, 24% positive containing two or three different The innocua (56%) welshimeri (43%) groups predominant...
Journal Article The use of multiplex PCR reactions to characterize populations lactic acid bacteria associated with meat spoilage Get access C.K. Yost, Yost Agriculture and Agri‐Food Canada, Lacombe Research Centre, Lacombe, Alberta, Canada 6000 C E Trail, T4L 1W1 (e‐mail: yostc@em.agr.ca). Search for other works by this author on: Oxford Academic Google Scholar F.M. Nattress Letters in Applied Microbiology, Volume 31, Issue 2, 1 August 2000, Pages 129–133,...
ABSTRACT We report the curing of 1,360-kb megaplasmid pRme2011a from Sinorhizobium meliloti strain Rm2011. With a positive selection strategy that utilized Tn 5 B12-S containing sacB gene, we were able to cure this replicon by successive rounds selecting for deletion formation in vivo. Subsequent Southern blot, Eckhardt gel, and pulsed-field gel electrophoresis analyses consistent with hypothesis resultant was indeed missing pRme2011a. The cured derivative grew as well wild-type both complex...
A wastewater treatment plant (WWTP) is an environment high in nutrient concentration with diverse bacterial populations and can provide ideal for the proliferation of mobile elements such as plasmids. WWTPs have also been identified reservoirs antibiotic resistance genes that are associated human pathogens. The objectives this study were to isolate characterize self-transmissible or mobilizable plasmids effluent from WWTP. An enrichment culture approach designed capture conferring...
Delftia is a diverse betaproteobacterial genus with many strains having agricultural and industrial relevance, including plant-growth promotion, bioremediation of hydrocarbon-contaminated soils, heavy metal immobilization. spp. are broadly distributed in the environment, have been isolated from plant hosts as well healthy diseased animal hosts, yet genetic basis this ecological versatility has not characterized. Here, we present phylogenomic comparison published genomes show that divided...
Cosmids carrying genes involved in utilization of rhamnose, sorbitol, and adonitol were isolated from a genomic library Rhizobium leguminosarum by complementation plasmid-cured derivatives strain Rlt100 that unable to grow on these carbon sources. Transposon mutagenesis was used identify regions each cosmid necessary for catabolism the respective source; partial DNA sequencing, as well analysis gene fusions created with transposon Tn5-B20, helped determine orientation possible function...
Methyl-accepting chemotaxis proteins (MCPs) play important roles in the chemotactic response of many bacteria. Oligonucleotide primers designed to amplify conserved signalling domain MCPs by PCR were used identify potential MCP-encoding genes Rhizobium leguminosarum. Using a PCR-derived probe created from these genomic library R. leguminosarum VF39SM was screened; at least five putative (termed mcpB mcpF) identified and isolated library. One (mcpC) is located on one indigenous plasmids...
A genetic locus encoding erythritol uptake and catabolism genes was identified in Rhizobium leguminosarum bv. viciae, shown to be plasmid encoded a wide range of R. strains. Tn5-B22 mutant (19B-3) unable grow on isolated from library strain VF39SM. The mutated gene eryF cloned partially sequenced, determined have high homology permease ABC transporters. cosmid complementing the mutation (pCos42) carry all necessary restore ability VF39SM cured pRleVF39f. In genomic DNA sequence 3841, linked...
Antibiotic resistance plasmids found in wastewater treatment plants (WWTPs) may represent a threat to public health if they are readily disseminated into the environment and ultimately pathogenic bacteria. The environments provide an ideal ecosystem for development evolution of antibiotic plasmids. Selective pressures to toxic compounds, high organic content bacterial diversity promotes gene exchange mechanisms involving interactions of conjugative with chromosomes, integrons transposons...
Vertebrate metamorphosis is often marked by dramatic morphological and physiological changes of the alimentary tract, along with major shifts in diet following development from larva to adult. Little known about how these developmental impact gut microbiome host organism. The sea lamprey (Petromyzon marinus) a sedentary filter-feeding free-swimming sanguivorous parasite characterized all organ systems. transformation canal includes closure larval esophagus physical isolation pharynx...